Biochemical Genetics

, Volume 27, Issue 9–10, pp 541–550 | Cite as

Genetic and synteny mapping of parathyroid hormone and beta hemoglobin in cattle

  • Marla E. Foreman
  • James E. Womack


Parathyroid hormone and the beta hemoglobin gene cluster, which are closely linked on human chromosome 11p15, were localized to bovine syntenic group (U7) with the gene for catalase by the use of bovine × hamster hybrid somatic cells. Restriction fragment length polymorphisms (RFLPs) were followed through informative pedigrees to determine a linkage map distance of 15.6±5.4 cM between the parathyroid hormone and hemoglobin genes. Allelic frequencies of the DNA fragment were compared in a small sampling of cattle from five different breeds.

Key words

cattle gene mapping parathyroid hormone beta hemoglobin catalase restriction fragment length polymorphism 


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  1. Antonarakis, S. E., Phillips, J. A., III, Mallonee, R. L., Kazazian, H. H., Jr., Fearon, E. R., Waber, P. G., Kronenberg, H. M., Ullrich, A., and Meyers, D. A. (1983). Beta-globin locus is linked to the parathyroid hormone (PTH) locus and lies between the insulin and PTH loci in man.Proc. Natl. Acad. Sci. USA 806615.PubMedGoogle Scholar
  2. Bernards, R., and Flavell, R. A. (1980). Physical mapping of the globin gene in hereditary persistance of foetal hemoglobin (HPFH).Nucl. Acids Res. 81521.PubMedGoogle Scholar
  3. Blin, N., and Stafford, D. W. (1976). Isolation of high molecular weight DNA.Nucl. Acids Res. 32303.PubMedGoogle Scholar
  4. Chaganti, R. S. K., Jhanwar, S. C., Antonarakis, S. E., and Hayward, W. S. (1985). Germ-line chromosomal localization of genes in chromosome 11p linkage: Parathyroid hormone, β-globin, c-Ha-ras-1, and insulin.Somat. Cell Mol. Genet. 11197.PubMedGoogle Scholar
  5. Deisseroth, A., Nienhuis, A., Lawrence, J., Giles, R., Turner, P., and Ruddle, F. H. (1978). Chromosomal localization of humanB globin gene on human chromosome 11 in somatic cell hybrids.Proc. Natl. Acad. Sci. USA 751456.PubMedGoogle Scholar
  6. Efremov, G., and Braend, M. (1965). Differences in cattle globins.Biochem. J. 97867.PubMedGoogle Scholar
  7. Feinberg, A. P., and Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Anal. Biochem. 1326.PubMedGoogle Scholar
  8. Fries, R., Hediger, R., and Stranzinger, G. (1988). The loci for parathyroid hormone and beta-globin are closely linked and map to chromosome 15 in cattle.Genomics 3302.PubMedGoogle Scholar
  9. Lalley, P. A., Sakaguchi, A. Y., Eddy, R. L., Honey, N. H., Bell, G. I., Shen, L.-P., Rutter, W. J., Jacobs, J. W., Heinrich, G., Chin, W. W., and Naylor, S. L. (1987). Mapping polypeptide hormone genes in the mouse: Somatostatin, glucagon, calcitonin, and parathyroid hormone.Cytogenet. Cell Genet. 4492.PubMedGoogle Scholar
  10. Lebo, R. V., Cheung, M. C., Bruce, B. D., Riccardi, V. M., Kao, F.-T., and Kan, Y. W. (1985). Mapping parathyroid hormone,B-globin, insulin, and LDH-A genes within the human chromosome 11 short arm by spot blotting sorted chromosomes.Hum. Genet. 69316.PubMedGoogle Scholar
  11. Levan, G., Szpirer, J., Szpirer, C., and Yoshida, M. C. (1986). Gene map of the rat.Rat. Newslett. 173.Google Scholar
  12. Ma, N. S. F., Gerhard, D. S., Housman, D. E., Orkin, S., and Bruns, G. (1986). Owl monkey gene mapping: The assignment of gene loci for catalase,beta globin gene cluster, HRAS1, insulin, and parathyroid hormone.Cytogenet. Cell Genet. 4357.PubMedGoogle Scholar
  13. Malcolm, S., Barton, P., Murphy, C., and Ferguson-Smith, M. A. (1981). Chromosomal localization of a single copy gene byin situ hybridization—HumanB-globin genes on the short arm of chromosome 11.Ann. Hum. Genet. 45135.PubMedGoogle Scholar
  14. Mayer, H., Breyel, E., Bostock, C., and Schmidtke, J. (1983). Assignment of the human parathyroid hormone gene to chromosome 11.Hum. Genet. 64283.PubMedGoogle Scholar
  15. Naylor, S. L., Sakaguchi, A. Y., Szoka, P., Hendy, G. N., Kronenberg, H. M., Rich, A., and Shows, T. B. (1983). Human parathyroid hormone gene (PTH) is on short arm of chromosome 11.Somat. Cell Genet. 9609.PubMedGoogle Scholar
  16. Reid, D. H., and Parsons, P. A. (1963). Sex of parent and variation of recombination with age in the mouse.Heredity 18107.PubMedGoogle Scholar
  17. Schimenti, J. C., and Duncan, C. H. (1985). Structure and organization of the bovineB-globin genes.Mol. Biol. Evol. 2514.PubMedGoogle Scholar
  18. Scott, A. F., Phillips, J. A., III, and Migeon, B. R. (1979). DNA restriction endonuclease analysis for localization of human beta-and delta-globin genes on chromosome 11.Proc. Natl. Acad. Sci. USA 764563.PubMedGoogle Scholar
  19. Southern, E. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis.J. Mol. Biol. 98503.PubMedGoogle Scholar
  20. Stenman, G., and Sager, R. (1987). Genetic analysis of tumorigenesis: A conserved region in the human and Chinese hamster genomes contains genetically identified tumor-suppressor genes.Proc. Natl. Acad. Sci. USA 849099.PubMedGoogle Scholar
  21. Weaver, C. A., Gordon, D. F., Kissel, M. S., Mead, D. A., and Kemper, B. (1984). Isolation and complete nucleotide sequence of the gene for bovine parathyroid hormone.Gene 28319.PubMedGoogle Scholar
  22. Womack, J. E., and Moll, Y. D. (1986). Gene map of the cow: Conservation of linkage with mouse and man.J. Hered. 772.PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Marla E. Foreman
    • 1
  • James E. Womack
    • 1
  1. 1.Department of Veterinary Pathology, College of Veterinary MedicineTexas A&M UniversityCollege Station

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