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Biochemical Genetics

, Volume 34, Issue 3–4, pp 109–116 | Cite as

Molecular mechanism of null expression of aldehyde dehydrogenase-1 in rat liver

  • James Chen
  • Yuchio Yanagawa
  • Akira Yoshida
Article

Abstract

In isozyme systems in general, the pattern of tissue-dependent expression of a given type of isozyme is uniform in various mammalian species. In contrast, a major cytosolic aldehyde dehydrogenase isozyme, termed ALDH1, which is strongly expressed in the livers of humans and other mammals, is hardly detectable in rat liver. Thirteen nucleotides existing in the 5′-promoter region of human, marmoset, and mouseALDH1 genes are absent in the four rat strains examined. When the 13 nucleotides were deleted from a chloramphenicol acetyltransferase expression construct, which contained the 5′-promoter region of the humanALDH1 gene and a low-background promoterless chloramphenicol acetyltransferase expression vector, the expression activity was severely diminished in human hepatic cells. Thus, deletion of the 13 nucleotides in the promoter region of the gene can account for the lack of ALDH1 expression in rat liver.

Key words

aldehyde dehydrogenase isozyme transcriptional regulation tissue-dependent expression 

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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • James Chen
    • 1
  • Yuchio Yanagawa
    • 1
  • Akira Yoshida
    • 1
  1. 1.Department of Biochemical Genetics, Beckman ResearchInstitute of the City of HopeDuarte

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