Summary
The cell in the human antral mucosa containing gastrin was identified with immunofluorescent methods using antibodies to synthetic human Gastrin (SHG 2–17). Light microscopic examination of the antral mucosa showed a chronic atrophic gastritis with intestinal metaplasia in most cases. The numerous Gastrin-cells demonstrated a specific immunofluorescence. The Gastrin-cells, however, did not stain on identical sections by the argyrophilic procedure of Grimelius, nor by lead-haematoxylin, carbodiimide reaction or diazonium method. These results confirm the correlative studies of McGuigan and Greider, who demonstrated that the Gastrin-cell in the hog antral mucosa did not stain by argyrophilic or diazonium methods. The Ec-cells with formaldehyde-induced fluorescence are stained by the diazonium method. The G-cells are defined by cytochemical reactions, the Gastrin-cells by immunochemical techniques. The argyrophilic G-cells and the Gastrin-cells are probably two different types of endocrine cells.
Zusammenfassung
Mit einem Antiserum gegen synthetisches Humangastrin (SHG 2–17) wurden die Gastrinzellen in der Antrumschleimhaut des Menschen immunhistologisch dargestellt. Histologisch zeigte die Magenschleimhaut in den meisten Fällen das Bild einer chronisch-atrophischen Gastritis mit enteraler Metaplasie im Antrumbereich. Mit der Immunfluoreszenz waren zahlreiche Gastrinzellen nachweisbar, die sich auf den identischen Schnitten jedoch weder mit der Versilberung nach Grimelius noch mit der Bleihämatoxylin-, der Carbodiimid-oder der Diazoniumreaktion darstellen ließen. Diese Befunde stimmen mit den Ergebnissen von McGuigan und Greider überein, die in der Antrumschleimhaut vom Schwein gleichfalls keine Korrelation der Gastrinzellen zu argyrophilen Zellen nachweisen konnten. Die Ec-Zellen mit Formalin-induzierter Fluorescenz korrelieren zur Diazoniumreaktion. Ein Vergleich der cytochemisch definierten G-Zelle mit den Gastrinzellen spricht für zwei unterschiedliche Zelltypen mit endokriner Aktivität.
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Literatur
Anderson, J. C., Barton, M. A., Gregory, R. A., Hardy, P. M., Kenner, G. W., MacLeod, J. K., Preston, J., Sheppard, R. C., Morley, J. S.: The antral hormone gastrin. II. Synthesis of gastrin. Nature (Lond.) 204, 933–934 (1964).
Bently, P. H., Kenner, G. W., Sheppard, R. C.: Structures of human gastrins I and II.. Nature (Lond.) 209, 583–585 (1966).
Bromé, A., Fyrö, B., Olbe, L.: Localization of gastrin activity in the gastric antrum. Acta physiol. scand. 74, 331–339 (1968).
Bussolati, G., Pearse, A. G. E.: Immunofluorescent localization of the gastrin-secreting G cells in the pyloric antrum of the pig. Histochemie 21, 1–4 (1970).
Byrnes, D. J., Young, J. D., Chisholm, D. J., Lazarus, L.: Serum gastrin in patients with peptic ulceration. Brit. med. J. 1970 II, 626–628.
Carvalheira, A. F., Welsch, U., Pearse, A. G. E.: Cytochemical and ultrastructural observations on the argentaffin and argyrophil cells of the gastro-intestinal tract in mammals, and their place in the APUD series of polypeptidesecreting cells. Histochemie 14, 33–46 (1968).
Crean, G. P., Marshall, M. W., Rumsey, R. D. E.: Parietal cell hyperplasia induced by the administration of pentagastrin (ICI 5,123) to rats. Gastroenterology 57, 147–155 (1969).
Dedmon, R. E., Holmes, A. W., Deinhardt, F.: Preparation of fluorescein isothiocyanate-labeled y-globulin by dialysis, gel filtration and ion-exchange chromatography in combination. J. Bact. 89, 734–739 (1965).
Forssmann, W. G.: Ultrastructure of hormone-producing cells of the upper gastrointestinal tract. In: Origin, chemistry, physiology, and pathophysiology of the gastrointestinal hormones, ed. Creutzfeldt. Stuttgart: W. Schattauer 1970.
—— Orci, L.: Zur Endokrinologie der Pylorusschleimhaut. I. Experimentelle Beeinflussung der Gastrinzelle. In: 15. Symp. Dtsch. Ges. Endokrinol. 1969, S. 408–411 Berlin-Heidelberg-New York: Springer 1969a.
—— —— Pictet, R., Renold, A. E., Rouiller, C.: The endocrine cells in the epithelium of the gastrointestinal mucosa of the rat. J. Cell Biol. 40, 692–715 (1969b)
Friesen, S. R., Bolinger, R. E., Pearse, A. G. E., McGuigan, J. E.: Serum gastrin levels in malignant Zollinger-Ellison syndrome after total gastrectomy and hypophysectomy. Ann. Surg. 172, 505–521 (1970).
Geyer, G.: Histochemischer Nachweis von Carboxylgruppen mit der Carbodiimidreaktion. Acta histochem. (Jena) 19, 73–77 (1964).
Gregory, H., Hardy, P. M., Jones, D. S., Kenner, G. W., Sheppard, R. C.: The antral hormone gastrin: structure of gastrin. Nature (Lond.) 204, 931–933 (1964b).
Gregory, R. A., Tracy, H. J.: The preparation and properties of gastrin. J. Physiol. (Lond.) 156, 523–534 (1961).
—— —— The constitution and properties of two gastrins extracted from hog antral mucosa. Gut 5, 103–114 (1964a).
—— —— Grossman, M. I.: Human gastrin: isolation, structure and synthesis. Isolation of two gastrins from human antral mucosa. Nature (Lond.) 209, 583–585 (1966).
Grimelius, L.: A modified silver protein method for studying the argyrophil cells of the islets of Langerhans. In: The structure and metabolism of pancreatic islets, ed. Brolin, S. E., Hellman, B., Knutson, H. Oxford: Pergamon 1964.
—— A silver nitrate stain for a2 cells in human pancreatic islets. Acta Soc. Med. upsalien. 73, 243–270 (1968).
Hansky, J., Cain, M. D.: Radioimmunoassay of gastrin in human serum. Lancer 1969 II, 1388–1390.
Hellerström, C., Hellman, B.: Some aspects of silver impregnation of the islets of Langerhans in the rat. Acta endocr. (Kbh.) 35, 518–532 (1960).
Lomský, R., Langr, F., Vortel, V.: Immunohistochemical demonstration of gastrin in mammalian islets of Langerhans. Nature (Lond.) 223, 618–619 (1969).
McGuigan, J. E.: Immunochemical studies with synthetic human gastrin. Gastroeneterology 54, 1005–1011 (1968a).
—— Gastric mucosal intracellular localization of gastrin by immunofluorescence. Gastroenterology 55, 315–327 (1968b).
—— On antibodies to Gastrin: concerning their production, behavioural characteristics, and uses. Gut 11, 363–367 (1970).
—— Greider, M. H.: Correlative immunochemical and light microscopic studies of the Gastrin cell in the antral mucosa. Gastroenterology 60, 223–236 (1971).
—— Trudeau, W. L.: Immunochemical measurement of elevated levels of gastrin in the serum of patients with pancreatic tumors of the Zollinger-Ellison variety. New Engl. J. Med. 278, 1308–1313 (1968).
—— —— Studies with antibodies to Gastrin. Radioimmunoassay in human serum and physiological studies. Gastroenterology 58, 139–150 (1970a).
—— —— Serum Gastrin concentrations in pernicious anemia. New Engl. J. Med. 282, 358–360 (1970b).
Nakane, P. K.: Classifications of anterior pituitary cell types with immunoenzyme histochemistry. J. Histochem. Cytochem. 18, 9–20 1970).
—— Pierce, G. B.: Encyme-labeled antibodies for the light and electron microscopic localization of tissue antigens. J. Cell Biol. 33, 307–318 (1967).
Pearse, A. G. E.: Histochemistry. London: Churchill 1960.
—— The cytochemistry and ultrastructure of polypeptide homone-producing cells of the APUD series and the embryologic, physiologic and pathologic implications of the concept. J. Histochem. Cytochem. 17, 303–313 (1969).
—— Bussolati, G.: Immunofluorescence studies of the distributin of gastrin cells in different clinical states. Gut 11, 646–648 (1970a).
—— Coulling, I., Weavers, B., Friesen, S.: The endocrine polypeptide cells of the human stomach, duodenum and jejunum. Gut 11, 649–658 (1970b).
Solcia, E., Capella, C., Vassallo, G.: Lead-haematoxylin as a stain for endocrine cells. Histochemie 20, 116–126 (1969b).
—— Sampietro, R.: Cytologic observations on the pancreatic islets with reference to some endocrine-like cells of the gastrointestinal mucosa. Z. Zellforsch. 68, 689–698 (1965).
—— Vassallo, G., Capella, C.: Selective staining of endocrine cells by basic dyes after acid hydrolysis. Stain Technol. 43, 257–263 (1968).
—— —— —— Studies on the pyloric mucosa, the probable site of gastrin secretion. Gut 10, 379–388 (1969a).
Solcia, E., Vassallo, G., Capella, C.: Cytology and cytochemistry of hormone producing cells of the upper gastrointestina tract. In: Origin, chemistry, physiology, and pathophysiology of the gastrointestinal hormones, ed. Creutzfeldt, W. Stuttgart: Schattauer 1970.
—— —— Sampietro, R.: Endocrine cells in the antropyloric mucosa of the stomach. Z. Zellforsch. 81, 474–486 (1967).
Trudeau, W. L., McGuigan, J. E.: Serum gastrin levels in patients with peptic ulcer disease. Gastroenterology 59, 6–12 (1970).
Vassallo, G., Capella, C., Solcia, E.: Grimelius' silver stain for endocrine cell granules as shown by electron microscopy. Stain Technol. 46, 7–13 (1971).
—— Solcia, E., Capella, C.: Licht and electron microscopic identification of several types of endocrine cells in the gastrointestinal mucosa of the cat. Z. Zellforsch. 98, 333–356 (1969).
Yalow, R. S., Berson, S. A.: Radioimmunoassay of gastrin. Gastroenterology 58, 1–14 (1970).
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Mitschke, H. Vergleichende immunhistologische und cytochemische Untersuchungen der Gastrinzellen beim Menschen. Virchows Arch. Abt. A Path. Anat. 353, 347–359 (1971). https://doi.org/10.1007/BF00549047
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DOI: https://doi.org/10.1007/BF00549047