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Morphologische Untersuchungen am Pankreas der Ratte im Kaliummangel unter besonderer Berücksichtigung der Langerhansschen Inseln

Morphological studies of the pancreas of rats in potassium deficiency, with special reference to the islets of Langerhans

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Summary

The morphology of the pancreas of rats in severe potassium depletion was studied following 18 hours of fasting. While the exocrine pancreas showed no differences from control animals, the A-cells in the islets of Langerhans of the control animals were decreased in size and degranulated following the fast. In potassium depletion this did not happen; on the contrary the A-cells seemed enlarged. The implications for glucagon secretion and electrolyte metabolism are discussed, with special reference to whether the secretion of glucagon by the A-cells is impaired in potassium depletion.

Zusammenfassung

Bei Ratten im Kaliummangel wurde nach 18stündigem Fasten eine Vergrößerung der A-Zellen in den Langerhansschen Inseln des Pankreas gefunden, während Normaltiere danach eine Degranulierung und Verkleinerung der A-Zellen zeigten. Beziehungen im Hinblick auf den Glucagonstoffwechsel einerseits, den Elektrolytstoffwechsel andererseits werden diskutiert, insbesondere, ob ein durch Kaliummangel hervorgerufener Sekretionsstop für den histologischen Befund eine Rolle spielen könnte.

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Literatur

  • Adams, C. W. M.: A histochemical method for tryptophan applicable to formalin fixed tissues. J. Path. Bact. 80, 442–445 (1960).

    Google Scholar 

  • Alm, G., Hellman, B.: Distribution of the two types of a cells in the pancreatic islets of some mammalian species. Acta endocr. (Kbh.) 46, 307–316 (1964).

    Google Scholar 

  • Bargmann, W.: Funktionelle Morphologie des inkretorischen Regulationssystems, V, 8. Der Inselapparat der Bauchspeicheldrüse. In: Handbuch der Allgemeinen Pathologie, Bd. VIII, Teil 1, S. 61–72. Berlin-Heidelberg-New York: Springer 1971.

    Google Scholar 

  • Bartelheimer, H. K., Losert, W., Senft, G., Sitt, R.: Störungen des Kohlehydratstoffwechsels im Kaliummangel. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 258, 391–408 (1967).

    Google Scholar 

  • Berg, G.: Histologische Labortechnik. München: J. F. Lehmanns 1972.

    Google Scholar 

  • Burkhardt, L.: Inselneubildung im Pankreas bei Stenose des Ausführungsganges durch Pankreaskopfcarcinom. Virchows Arch. path. Anat. 296, 655–665 (1936).

    Google Scholar 

  • Conn, J. W.: Hypertension, the potassium ion and impaired carbohydrate tolerance. New Engl. J. Med. 273, 1135–1143 (1965).

    Google Scholar 

  • Creutzfeldt, W.: Die pathologische Morphologie der Langerhans'schen Inseln im Experiment, besonders beim experimentellen Diabetes. Verh. dtsch. path. Ges. 42, 85–106 (1958).

    Google Scholar 

  • Creutzfeldt, W., Theodossiou, A.: Die Relation der A- und B-Zellen in den Pankreasinseln bei Nichtdiabetikern und Diabetikern. Beitr. path. Anat. 117, 235–252 (1957).

    Google Scholar 

  • Dawson, J.: The endocrine cells of the gastrointestinal tract. Histochem. J. 2, 527–549 (1970).

    Google Scholar 

  • Doerr, W.: Spezielle pathologische Anatomie II, S. 329–339. Heidelberg-New York: Springer 1970.

    Google Scholar 

  • Doerr, W., Becker, V.: Die Bauchspeicheldrüse. In: Pathologie der Laboratoriumstiere (eds. P. Cohrs, R. Jaffè, H. Meessen), Bd. I. Berlin-Göttingen-Heidelberg: Springer 1958.

    Google Scholar 

  • Faller, A.: Elektronenmikroskopische Differenzierung verschiedener Inselzelltypen im Pankreas normaler Albinoratten. Z. Zellforsch. 97, 226–248 (1969).

    Google Scholar 

  • Ferner, H.: Das Inselsystem des Pankreas. Stuttgart: G. Thieme 1952.

    Google Scholar 

  • Ferner, H.: Die orthologische Histologie und Cytologie der Langerhans'schen Inseln. Verh. dtsch. path. Ges. 42, 25–41 (1958).

    Google Scholar 

  • Ferner, H., Runge, W.: Die Wirkung des Synthalin A auf das A-Zellsystem des Inselorgans bei der Ratte in den ersten Lebenstagen. Z. Zellforsch. 43, 1–7 (1955).

    Google Scholar 

  • Follis, R. H., Orent-Keiles, E., McCollumm, E. V.: The production of cardiac and renal lesions in rats by a diet extremely low in potassium. Amer. J. Path. 18, 29–40 (1942).

    Google Scholar 

  • Gepts, W.: Die histopathologischen Veränderungen der Langerhans'schen Inseln und ihre Bedeutung in der Frage der Pathogenese des menschlichen Diabetes. Endokrinologie 36, 185–211 (1958).

    Google Scholar 

  • Gorden, Ph., Sherman, B. M., Sinopoulos, A. P.: Glucose intolerance with hypokalemia: An increased proportion of circulating proinsulin-like components. J. clin. Endocr. 34, 235–240 (1972).

    Google Scholar 

  • Greider, M. H., Howell, S. L., Lacy, P. E.: Isolation and properties of secretory granules from rat islets of Langerhans. J. Cell. Biol. 41, 162–166 (1969).

    Google Scholar 

  • Greider, M. H., McGuigan, J. E.: Cellular localisation of gastrin in the human pancreas. Diabetes 20, 389–396 (1971).

    Google Scholar 

  • Hamperl, H.: Über argyrophile Zellen. Virchows Arch. path. Anat. 321, 482–507 (1952).

    Google Scholar 

  • Hellerström, C., Hellman, B.: Some aspects of silver inpregnation of the islets of Langerhans in the rat. Acta endocrin. (Kbh.) 35, 518–532 (1960).

    Google Scholar 

  • Hellerström, C., Hellman, B.: Reactions of the two types of A cells in the islets of Langerhans after administration of glucagon. Acta endocrin. (Kbh.) 41, 116–122 (1962).

    Google Scholar 

  • Hellerström, C., Hellman, B., Petersson, B., Alm, G.: The two types of pancreatic A-cells and their relation to the glucagon secretion. In: S. E. Brolin, B. Hellman, H. Knutson (eds.). The structure and metabolism of the pancreatic islets, S. 117–130. Oxford: Pergamon Press 1964.

    Google Scholar 

  • Hellman, B.: Methodical aspects on the differential cell count of the islet tissue in the rat. Acta path. microbiol. scand. 45, 336–346 (1959a).

    Google Scholar 

  • Hellman, B.: The volumetric distribution of the pancreatic islet tissue in young and old rats. Acta endocrin. (Kbh.) 31, 91–106 (1959b).

    Google Scholar 

  • Hellman, B.: The effect of ageing on the number of the islets of Langerhans in the rat. Acta endocrin. (Kbh.) 32, 78–91 (1959c).

    Google Scholar 

  • Hellman, B.: The effect of ageing on the total volumes of the A and B cells in the islets of Langerhans of the rat. Acta endocrin. (Kbh.) 32, 92–112 (1959d).

    Google Scholar 

  • Hellman, B.: The relation between age and the B/A cell ratio in the islet tissue of the rat. Acta endocrin. (Kbh.) 31, 80–90 (1959e).

    Google Scholar 

  • Hellman, B.: The numerial distribution of the islets of Langerhans at different ages of the rat. Acta endocrin. (Kbh.) 32, 63–77 (1959f).

    Google Scholar 

  • Hellman, B.: Nuclear differences between the argyrophil (=A1) and non argyrophil (=A2) pancreatic A cells in the duck. Acta endocrin. (Kbh.) 36, 603–608 (1961).

    Google Scholar 

  • Hellman, B., Hellerström, C.: Diurnal changes in the function of the pancreatic islets of rats as indicated by nuclear size in the islet cells. Acta endocrin. (Kbh.) 31, 267–281 (1959).

    Google Scholar 

  • Hellman, B., Petersson, B., Hellerström, C.: The growth pattern of the endocrine pancreas in mammals. In: S. E. Brolin, B. Hellman, H. Knutson (eds.). The structure and metabolism of the pancreatic islets, S. 45–60. Oxford: Pergamon Press 1964.

    Google Scholar 

  • Hiatt, N., Davidson, M. B., Bonorris, G.: The effect of potassium chloryde infusion on insulin secretion in vivo. Hormone and metabolic Res. 4, 64–68 (1972).

    Google Scholar 

  • Hokfelt, B., Hultquist, G.: The influence of hypoglycemia on the histologic structure of the alpha cells in the pancreas. Acta physiol. scand 43, 8–14 (1958).

    Google Scholar 

  • Howell, S. L., Taylor, K. W.: Potassium ions and the secretion of insulin by islets of Langerhans incubated in vitro. Biochem. J. 108, 17–24 (1968).

    Google Scholar 

  • Hultquist, G. T., Dahlen, M., Helander, C. G.: Über die Technik bei Darstellung und Zählung der sog. Silberzellen in den Langerhans'schen Inseln. Schweiz. Z. Path. 11, 570–589 (1948).

    Google Scholar 

  • Kaess, H., Ehlers, W., Burkhardt, A.: Distribution and insulin content in the pancreas of potassium deficient rats. (In Vorbereitung).

  • Kaess, H., Schlierf, G., Ehlers, W., von Mikulicz-Radecki, J. G., Hassenstein, P., Walter, K., Brech, W., Hengstman, J.: The carbohydrate metabolism of normal subjects during potassium depletion. Diabetologia 7, 82–86 (1971).

    Google Scholar 

  • Korps, W., LeCompte, Ph. M.: The nature and function of the alpha cells of the pancreas. Diabetes 4, 347–366 (1955).

    Google Scholar 

  • Kracht, J.: Funktionszustände des Inselsystems. Verh. dtsch. path. Ges. 42, 116–121 (1958a).

    Google Scholar 

  • Kracht, J.: Morphologische Kriterien zur Beurteilung der Inselaktivität. Endokrinologie 36, 146–158 (1958b).

    Google Scholar 

  • Lazarus, S. S., Volk, B. W.: The effect of protracted glucagon administration on blood glucose and on pancreatic morphology. Endocrinology 63, 359–371 (1958).

    Google Scholar 

  • Marzotko, D., Scharf, J.-H., Farr, I., Opitz, Chr., Schmidt, R., Liedel, H., Wetzig, H.: Quantitativ- und quantitativ-histologische Befunde am Inselorgan adulter Albinoratten nach Alteration endokriner Regelkreise. Gegenbaurs morph. Jb. 116, 186–207 (1971).

    Google Scholar 

  • Mikami, S., Mutoh, K.: Light and electron-microscopic studies of the pancreatic islet cells in the chicken under normal and experimental conditions. Z. Zellforsch. 116, 205–227 (1971).

    Google Scholar 

  • Mondon, C. E., Burton, S. A., Grodsky, G. M., Ishida, T.: Glucose tolerance and insulin response of potassium deficient rat and isolated liver. Amer. J. Physiol. 215, 779–787 (1968).

    Google Scholar 

  • Müller, W. A., Faloona, G. R., Aguilar-Parada, E., Unger, R. H.: Abnormal alpha-cell function in diabetes. New Engl. J. Med. 283, 109–115 (1970).

    Google Scholar 

  • Müller, W. A., Faloona, G. R., Unger, R. H.: The effect of experimental insulindeficiency on glucagon secretion. J. clin. Invest. 50, 1992–1999 (1971).

    Google Scholar 

  • Pfanzagl, J.: Allgemeine Methodenlehre der Statistik, Bd. II. Berlin: De Gruyter 1966.

    Google Scholar 

  • Polak, J. M., Bloom, S., Conling, J., Pearse, A. G. E.: Immunfluorescent localization of secretin in the canine duodenum. Gut 12, 605–610 (1971).

    Google Scholar 

  • Sagild, U., Andersen, V., Andreasen, P. B.: Glucose tolerance and insulin responsiveness in experimental potassium depletion. Acta med. scand. 169, 243–251 (1961).

    Google Scholar 

  • Seifert, G.: Die pathologische Morphologie der Langerhans'schen Inseln, besonders beim Diabetes mellitus des Menschen. Verh. dtsch. path. Ges. 42, 50–84 (1958).

    Google Scholar 

  • Smith, S. G., Black-Schaffer, B., Lasater, Th. E.: Potassium deficiency syndrome in the rat and the dog. Arch. Path. 49, 185–199 (1950).

    Google Scholar 

  • Unger, R. H., Aguilar-Parada, E., Müller, W. A., Eisentraut, A. M.: Studies of pancreatic alpha cell function in normal and diabetic subjects. J. clin. Invest. 49, 837–848 (1970).

    Google Scholar 

  • Wattenwyl, N. von: Neuere Differenzierungsmethoden der Langerhans'schen Inseln. Path. et Microbiol. (Basel) 27, 144–156 (1964).

    Google Scholar 

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Authors and Affiliations

  1. Pathologisches Institut der Universität Heidelberg und Medizinische Universitätsklinik Heidelberg, Germany

    A. Burkhardt, H. Kaess & W. Ehlers

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  1. A. Burkhardt
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  2. H. Kaess
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  3. W. Ehlers
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Burkhardt, A., Kaess, H. & Ehlers, W. Morphologische Untersuchungen am Pankreas der Ratte im Kaliummangel unter besonderer Berücksichtigung der Langerhansschen Inseln. Virchows Arch. Abt. A Path. Anat. 360, 57–70 (1973). https://doi.org/10.1007/BF00548658

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