Summary
In 192 liver needle biopsies prepared with Wachstein-Meisel method, we found a development of abnormal projections of bile canaliculi 95 times. Of 95 biopsies, 50 also revealed other types of canalicular abnormalities. From the intensity of changes seen 4 groups could be formed. The maximal changes appeared in complicated fatty liver, in cirrhosis and in chronic hepatitis. The changes described were particularly focal. The pathogenesis of the formation of projections is ascribed to the combined actions of several factors, mainly to hepatocellular damage with obstruction. The relationships of these morphologic changes to other histological findings in the liver are discussed.
Zusammenfassung
In 192, nach der Methode von Wachstein und Meisel bearbeiteten Leberpunktaten, wurde 95mal die Bildung von abnormalen Gallenkanälchenausläufern beobachtet. Bei den 95 Punktaten kamen 50mal auch andere Typen von Kanälchenabnormitäten vor. Mit Rücksicht auf deren Intensität wurden die vorwiegend herdförmigen Veränderungen in 4 Gruppen eingeteilt. Der Maximalbefund wurde bei komplizierter Leberverfettung, bei Cirrhosen und bei chronischer Hepatitis beobachtet. In der Pathogenese der Entstehung neuer Kanälchenausläufer wirken mehrere Faktoren, hauptsächlich die hepatocelluläre Beschädigung mit Obstruktion. Es werden die morphologischen Beziehungen zu den anderen feingeweblichen Befunden in der Leber angeführt.
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Literatur
Bhathal, P. S., Christie, G. S.: A fluorescence microscopic study of bile duct proliferation induced in Guinea pigs by α-naphthyl isothiocyanate. Lab. Invest. 20, 480–487 (1969).
Breitfellner, G., Holzner, J. H., Schumacher, A., Stefenelli, N.: Die elektronenoptischen und die nach enzymhistochemischer Darstellung der ATPase erkennbaren Veränderungen der Rattenleber bei experimenteller intrahepatischer Cholestase. Beitr. path. Anat. 134, 267–288 (1966).
Carruthers, J. S., Steiner, J. W.: Fine structure of terminal branches of the biliary tree. Arch. Path. 74, 117–126 (1962).
Desmet, V. J., Krstulović, B., Damme, B. von: Histochemical study of rat liver in alphanaphthyl isothiocyanate (ANIT) induced cholestasis. Amer. J. Path. 52, 401–421 (1968).
Holzner, J. H., Stefenelli, N., Wewalka, F. G.: Veränderungen an den Gallenkapillaren bei Fettleber. Wien. klin. Wschr. 74, 810–814 (1962).
—— —— —— Die Bedeutung enzymhistochemischer Methoden für die Unterscheidung verschiedener Formen der Cholostase. 2. Weltkongr. für Gastroenterologie. III. München, 1962. Basel-New York: Karger 1963.
—— —— —— Enzymhistochemische Veränderungen der Leber bei Hepatitis. Acta hepato-splenol. (Stuttg.) 13, 1–14 (1966).
Krstulovié, B., Damme, B. von, Desmet, V. J.: Comparative histochemical study of rat liver in bile-duct ligation and in alpha-naphthyl isothiocyanate (ANIT) intoxication. Amer. J. Path. 52, 423–436 (1968).
Lane, B. P., Becker, F. F.: Regeneration of the mammalian liver. II. Surface alterations during differentiation of the liver cell in preparation for cell division. Amer. J. Path. 48, 183–196 (1966).
Moses, H. L., Rosenthal, A. S., Beaver, D. L., Schuffman, S. S.: Lead ion and phosphatase histochemistry. II. Effect of adenosine triphosphate hydrolysis by lead ion on the histochemical localization of adenosine triphosphatase activity. J. Histochem. Cytochem. 14, 702–710 (1966).
Novikoff, A. B.: Enzyme localization with Wachstein-Meisel procedures; real or artifact. J. Histochem. Cytochem. 15, 353–354 (1967).
—— Drucker, J., Woo-Yung Shin, Goldfischer, S.: Further studies of the apparent adenosinetriphosphatase activity of cell membranes in formol-calcium-fixed tissues. J. Histochem. Cytochem. 9, 434–451 (1961).
Pavel, I., Câmpeanu, S., Bonaparte, H., Petrovici, A.: The ultrastructure of the hepatic intercellular spaces in certain diseases. Path. Microbiol. 34, 35–44 (1969).
Phillips, M. J., Steiner, J. W.: Electron microscopy of liver cells in cirrhotic nodules. I. The lateral cell membranes. Amer. J. Path. 46, 985–1005 (1965).
—— —— Electron microscopy of cirrhotic nodules. Tubularization of the parenchyma by biliary hepatocytes. Lab. Invest. 15, 801–817 (1966).
Rosenthal, A. S., Moses, H. L., Beaver, D. L., Schuffman, S. S.: Lead ion and phosphatase histochemistry. I. Nonenzymatic hydrolysis of nucleoside phosphates by lead ion. J. Histochem. Cytochem. 14, 698–701 (1966).
Safran, A. P., Schaffner, F.: Chronic passive congestion of the liver in man. Amer. J. Path. 50, 447–463 (1967).
Sandström, B., Westman, J.: Non-freezing light- and electron microscopic enzyme histochemistry by means of polyetylene glycol embedding. Histochemie 19, 181–183 (1969).
—— On the specificity of histochemistry demonstrable bile canalicular phosphatase activities. Histochemie 22, 316–323 (1970).
Schaffner, F.: Morphologic studies on bile secretion. Amer. J. dig. Dis. 10, 99–115 (1965).
Steiner, J. W., Carruthers, J. S.: Studies on the fine structure of the terminal branches of the biliary tree. Amer. J. Path. 39, 41–63 (1961).
Tanikawa, K.: Ultrastructural aspects of the liver and its disorders. Berlin-Heidelberg-New York: Springer 1968.
Wachstein, M.: Enzymatic histochemistry of the liver. Gastroenterology 37, 525–537 (1959).
—— Meisel, E.: Histochemistry of hepatic phosphatases at physiologic pH. With special reference to the demonstration of bile canaliculi. Amer. J. clin. Path. 27, 13–23 (1957).
Wasastjerna, C., Thodén, C. J., Ekelund, P., Haltia, K.: The bile canaliculi in cytological aspiration biopsies from patients with liver disorders. Scand. J. Gastroent. 5, 327–331 (1970).
Wills, E. J., Epstein, M. A.: Subcellular changes in surface adenosine triphosphatase activity of human liver in extrahepatic obstructive jaundice. Amer. J. Path. 49, 605–635 (1966).
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Horváth, A., Horváthová, J., Brixová, E. et al. Zur abnormalen Ausläuferbildung des Gallenkanälchennetzes in Leberpunktaten. Virchows Arch. Abt. A Path. Anat. 354, 35–43 (1971). https://doi.org/10.1007/BF00544105
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DOI: https://doi.org/10.1007/BF00544105