Advertisement

Virchows Archiv A

, Volume 361, Issue 1, pp 51–69 | Cite as

Ultrastrukturelle Differenzierung der Phäochromocytome

  • J. Cervós-Navarro
  • J. M. Bayer
  • H. Käser
Article

Zusammenfassung

6 operativ entfernte Phäochromocytome werden elektronenmikro-skopisch untersucht. 5 davon lassen sich trotz verschiedener Anteile unterschiedlicher Zelltypen in ihrem Aufbau als einheitliche Gruppe einordnen. Im 6. Tumor, dem einzigen, der praktisch nur Noradrenalin ausgeschieden hat, zeigt ein Teil der Tumorzellen Merkmale neuronaler Zellelemente. Die Haupttumorzellen aller 6 Fälle gemeinsam werden durch ihre innerhalb bestimmter Variationsbreiten konstanten Zahl phäochromer Granula gekennzeichnet. In einem Fall zeigten die phäochromen Granula in den einzelnen Zellen eine bestimmte Größe und einen bestimmten Kontrastreichtum. Die Haupttumorzellen in den übrigen Fällen zeigen alle möglichen Variationen der phäochromen Granula in ein- und demselben Zellelement. Die Vermischung der verschiedenen Typen phäochromer Granula macht eine Unterscheidung von Zellen nach ihrem Sekretionstyp unmöglich. Auch eine Unterscheidung zwischen speichernden Zellen konnte nicht festgestellt werden. Die auffallenden Unterschiede zwischen hellen und dunklen Zellen sind auf die verschiedene Dichte des Grundplasmas und nicht auf die phäochromen Granula zurückzuführen.

Ultrastructural differentiation of pheochromocytoma

Summary

Six surgically removed pheochromocytomas were examined by electron microscopy.

Despite the variety of cell types, five of the tumors were classed as noradrenalin-adrenalin-secreting on the basis of overall structure. Only one of the six tumors, through clinical investigation, was classified as essentially noradrenalin-secreting. Some cells of this tumor displayed the characteristics of neuronal elements.

In all six tumors examined the main tumor cells contained a nearly constant number of osmiophilic granula. In some cells of one of the noradrenalin-adrenalin-secreting tumors these granula were all about the same size and had a limited range of light and dark contrast. In the cells of the other tumors, however, the size and contrast of the granula varied. Because of this variation, it was impossible to differentiate between secreting and non-secreting cells or to classify them into synthesizing and storage cells. The difference between light and dark cells is due, not to the presence of osmiophilic granula, but to differences in the density of the basic substance.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Bachmann, R.: Die Nebenniere. In: Handbuch der mikroskopischen Anatomie des Menschen, Bd. VI, Teil 5. Berlin-Göttingen-Heidelberg: Springer 1954Google Scholar
  2. Bässler, R., Habighorst, L. V.: Vergleichende licht- und elektronenmikroskopische Untersuchungen am Nebennierenmark und Phäochromocytom. Beitr. path. Anat. 130, 446–488 (1964)Google Scholar
  3. Belt, A. E., Powell, T. O.: Clinical manifestations of the chromaffin cell tumors arising from the suprarenal medulla. Surg., Gynec. Obstet. 59, 9–24 (1934)Google Scholar
  4. Benedeczky, I.: Ultrastructural analysis of adrenaline resynthesis following insulin treatment. Acta morph. hung. Acta. Sci. 15, 23–37 (1967)Google Scholar
  5. Benedeczky, I., Lapis, K.: Vergleichende elektronenmikroskopische Untersuchungen am Nebennierenmark und Phäochromocytom des Menschen. Electron Microscopy of human adrenal medulla and pheochromocytoma. Beitr. path. Anat. 137, 403–438 (1968)Google Scholar
  6. Benedeczky, I., Puppi, A., Tigyi, A., Lissak, K.: Various cell types of the adrenal medulla. Nature (Lond.) 204, 591–592 (1964)Google Scholar
  7. Benedczky, I., Puppi, A., Tigyi, A., Lissak, K.: Various cell types in the adrenal medulla. Nature (Lond.) 209, 592–594 (1966)Google Scholar
  8. Bennett, H. S.: Cytological manifestation of secretion in the adrenal medulla of the cat. Amer. J. Anat. 69/3, 333–381 (1941)Google Scholar
  9. Blacklock, J. W. S., Ferguson, J. W., Mack, W. S., Shafar, J., Symington, T.: Phaeochromocytoma. Brit. J. Surg. 35, 179–197 (1947)Google Scholar
  10. Bretschneider, H. J., Schattenfroh, C., Schoebb, J. O.: Clinical aspects and morphology of different pheochromocytoma types. Langenbecks Arch. klin. Chir. 299, 665–692 (1962)Google Scholar
  11. Brown, W. J., Barajas, L., Latta, H.: The ultrastructure of the human adrenal medulla: with comparative studies of white rat. Anat. Rec. 169, 173–184 (1971)Google Scholar
  12. Brown, W. J., Barajas, L., Waisman, J., de Quattro, V.: Ultrastructural and biochemical correlates of adrenal extraadrenal pheochromocytoma. Cancer (Philad.) 29, 744–759 (1972)Google Scholar
  13. Bucciante, G., Meneghelli, V.: Sulla struttura del feocromocitoma analizzata al microscopio elettronico. Acta med patav. 24, 653 (1964)Google Scholar
  14. Cavallero, C.: I Simp. con discussione a tavola rotonda. Il Feocromocitoma. Chir. triven. 4, 115 (1964)Google Scholar
  15. Cervós-Navarro, J., Vazquez, J. J.: An electron microscopic study of meningiomas. Acta neuropath. (Berl.) 13, 301–323 (1969)Google Scholar
  16. Coupland, R. E.: Electron microscopic observations on the structure of the rat adrenal medulla. I. The ultrastructure and organization of chromaffin cells in the normal adrenal medulla. J. Anat. (Lond.) 99, 231–254 (1965a)Google Scholar
  17. Coupland, R. E., Hopwood, D.: Mechanism of a histochemical reaction differentiating between adrenaline and noradrenaline-storing cells in the electron microscope. Nature (Lond.) 209, 590–591 (1966)Google Scholar
  18. Coupland, R. E., Pyper, A. S., Hopwood, D.: A method for differentiating between noradrenaline- and adrenaline-storing cells in the light- and electron microscope. Nature (Lond.) 201, 1240–1242 (1964)Google Scholar
  19. Crout, J. R., Sjoerdsma, A.: Turnover and metabolism of catecholamines in patients with pheochromocytoma. J. clin. Invest. 43, 94–102 (1964)Google Scholar
  20. Cushing, H., Burt, W. S.: The transformation of a malignant paravertebral sympathicoblastoma into a benign ganglioneuroma. Amer. J. Path. 3, 203 (1927)Google Scholar
  21. Dietrich, A., Siegmund, H.: Die Nebenniere und das chromaffine System (Paraganglien, Steißdrüse, Karotisdrüse). In: Handbuch der speziellen pathol. Anatomie und Histologie, Bd. 8, S. 951–1089. Berlin-Göttingen-Heidelberg: Springer 1926Google Scholar
  22. Elfvin, L. G.: The fine structure of the cell surface of chromaffin cells in the rat adrenal medulla. J. Ultrastruct. Res. 12, 263–286 (1965a)Google Scholar
  23. Ewing, J.: Neoplastic diseases: a treatise on tumors, ed. 4, p. 839–840. Philadelphia: W. B. Saunders Co. 1940Google Scholar
  24. Gasser, G., Kühböck, J., Obiditsch-Mayer, I.: Pheochromocytoma under the clinical picture of chronic hypertension. Wien Z. Iun. Med. 46, 217–225 (1965)Google Scholar
  25. Goormaghtigh, N.: Surrénales et thermorégulation. Tests morphologiques d'activité médullosurrénale. Arch. Biol. (Liège) 41, 109–142 (1931)Google Scholar
  26. Greenberg, R., Rosenthal, I., Falk, G. S.: Electron microscopy of human tumors secreting catecholamines: correlation with biochemical data. J. Neuropath. exp. Neurol. 28, 475–500 (1969)Google Scholar
  27. Gusek, W., Fock, M.: Ultrastruktur hormondifferenter Phäochromocytome. Acta endocr. (Kbh.), Suppl. 159, 84 (1972)Google Scholar
  28. Herxheimer, G.: Über Tumoren des Nebennierenmarkes, insbesondere das Neuroblastoma sympathicum. Beitr. path. Anat. 57, 112 (1913)Google Scholar
  29. Hion, J. V.: Zur Histologie der Nebennieren bei erschöpften Tieren. Fol. neuropath. eston. 7, 178–189 (1927)Google Scholar
  30. Kleinschmidt, A., Schümann, H. J.: Strukturuntersuchungen über die Adrenalin und Noradrenalin speichernden Granula des Nebennierenmarks. Naunyn-Schmiedeberg Arch. exp. Path. Pharmak. 241, 260 (1961)Google Scholar
  31. Laumonier, R., Marche, Cl., Marche, J.: Etude ultrastructurale d'un phaeochromocytome. Ann. Anat. path. 13, 137–146 (1968)Google Scholar
  32. Lever, J. D.: Electron microscopic observations on the normal and denervated adrenal medulla of the rat. Endocrinology 57, 621–635 (1955)Google Scholar
  33. Luse, S.: Ultrastructural characteristics of normal and neoplastic cells. Progr. exp. Tumor Res. (Basel) 2, 1–35 (1961)Google Scholar
  34. Misugi, K., Misugi, N., Newton, W.A. Jr.: Fine structural study of neuroblastoma, ganglioneuroblastoma, and pheochromocytoma. Arch. Path. 86, 160–170 (1968)Google Scholar
  35. Moppert, J.: Zur Ultrastruktur der phaeochromen Zellen im Nebennierenmark der Ratte. Z. Zellforsch. 74, 32–44 (1966a)Google Scholar
  36. Morini, P.L., Fazzini, G.: Considerazioni sugli aspetti umorali, citochimici ed ultrastrutturali di un caso di feocromocitoma. Folia endocr. (Roma) 21, No 3 (1968)Google Scholar
  37. Page, L.B., Jacobi, G.A.: Catecholamine metabolism and storage granules in pheochromocytoma and neuroblastoma. Medicine (Baltimore) 43, 379–386 (1964)Google Scholar
  38. Ratzenhofer, M., Auböck, L.: Zur Kenntnis vom Feinbau und Sekretionsmechanismus der Phäochromocytome. On Ultrastructure and secretion of pheochromocytoma. Beitr. path. Anat. 137, 36–64 (1968)Google Scholar
  39. Rosenthal, I.M., Greenberg, R., Goldstein, R., Kathan, R., Cadkin, L.: Catecholamine metabolism in a pheochromocytoma. Correlation with electron micrographs. Amer. J. Dis. Child. 112, 389–395 (1966)Google Scholar
  40. Sherwin, R.P.: Histopathology of pheochromocytoma. Cancer (Philad.) 12, 861–877 (1959)Google Scholar
  41. Sherwin, R.P.: New aspects of the chromoreactions for the diagnosis of pheochromocytoma. Amer. J. clin. Path. 43, 200–206 (1965)Google Scholar
  42. Sherwin, R.P.: The adrenal medulla, paraganglia and related tissues. In: Endocrine pathology, J.M.B. Bloodworth Jr., ed., Baltimore: Williams & Wilkins 1968Google Scholar
  43. Steiner, J.W., Baglio, C.M.: Electron microscopy of the cytoplasm of parenchymal liver cells Naphthylisothiocyanate—induced cirrhosis. Lab. Invest. 12, 765 (1963)Google Scholar
  44. Steiner, J.W., Carruthers, J.S., Kalifat, S.R.: Observations on the fine structure of rat liver cells in extrahepatic cholestasis. Z. Zellforsch. 58, 141 (1962)Google Scholar
  45. Thoenes, W., Bannasch, P.: Elektronen- und lichtmikrokopische Untersuchungen am Cytoplasma der Leberzellen nach akuter und chronischer Thiocetamid-Vergiftung. Virchows Arch. path. Anat. 335, 556 (1962)Google Scholar
  46. Wahl, H.R., Craig, P.E.: Multiple tumors of the sympathetic nervous system. Report of a case showing a distinct ganglioneuroma, a neuroblastoma and a cystic calcifying ganglioneuroblastoma. Amer. J. Path. 14, 797 (1938)Google Scholar
  47. Wassermann, G., Tramezzani, J.H.: Separate distribution of adrenaline-and noradrenaline-secreting cells in the adrenal of snakes. Gen. comp. Endocr. 3, 480–489 (1963)Google Scholar
  48. Wood, J.G., Barrnett, J.R.: Histochemical demonstration of norepinephrine at a fine structural level. J. Histochem. Cytochem. 12, 197–209 (1964)Google Scholar
  49. Yates, R.D., Wood, J.G., Duncan, D.: Phase and electron microscopic observations on two cell types in the adrenal medulla of the syrian hamster. Tex. Rep. Biol. Med. 20, 494–502 (1962)Google Scholar
  50. Yokoyama, M.: An electron microscopic study of the human adrenal medulla and pheochromocytoma. J. Urol. Soc. Japan 57, 1095–1121 (1966)Google Scholar
  51. Yokoyama, M., Takayasu, H.: An electron microscopic study of the human adrenal medulla and pheochromocytoma. Urol. int. (Basel) 24, 79–95 (1969)Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • J. Cervós-Navarro
    • 1
    • 2
    • 3
  • J. M. Bayer
    • 1
    • 2
    • 3
  • H. Käser
    • 1
    • 2
    • 3
  1. 1.Institut für Neuropathologie der Freien Universität BerlinGermany
  2. 2.Chirurgische Klinik der Universität BonnGermany
  3. 3.Stoffwechsel-Abteilung der Schweizerischen Zentrale für klinische TumorforschungSwitzerland

Personalised recommendations