Summary
Occurrence and localization of the carcinoembryonic antigen (CEA) and of a perchloric acid-soluble antigen of normal colonic mucosa (NC) were compared in polyps and carcinomas of the colon using the indirect immunfluorescence technique. NC is located in the secretory part of the goblet cells and diffusely distributed in the mucus. Therefore, it can be considered as a marker of large-bowel mucus. NC is found in all mucus-producing tumors regardless of their degree of differentiation in intra- and extracellular mucus deposits. Tumor cells which closely resemble normal goblet cells produce and secrete NC. With respect to their NC content, there is no immunologically detectable qualitative difference between the secretions of normal and of neoplastic large bowel mucosa. It is clearly shown that production and secretion of CEA is independent of mucus production and secretion. In contrast to earlier results, CEA was found in polyps and in normal mucosa surrounding the carcinoma, confirming the observations of Burtin et al. (1972a).
Zusammenfassung
Auftreten und Lokalisation eines aus normaler Colonmucosa mit Perchlorsäure extrahierbaren Antigens (NC) in benignen und malignen Colontumoren wurden mittels indirekter Immunfluorescenz untersucht und mit dem carcinoembryonalen Antigen (CEA) verglichen. In der normalen Dickdarmschleimhaut trat das NC intracellulär im sekretorischen Anteil der Becherzellen, extracellulär diffus im Schleim auf. Es kann somit als Schleimantigen angesehen werden. In Tumoren fand es sich unabhängig vom Differenzierungsgrad immer im intra- und extracellulären Schleim. In hochdifferenzierten Tumoren wurde es reichlich in Zellen gefunden, die normalen Becherzellen ähnelten. Diese Zellen sezernierten NC ins Lumen. Bezüglich des NC verhält sich der Schleim verschiedener Tumoren und der normalen Schleimhaut gleich. Es zeigt sich deutlich, daß die vom Differenzierungsgrad des Tumors abhängige CEA-Produktion von der Schleimproduktion und vom Auftreten des NC unabhängig ist. Entgegen früheren Befunden konnte CEA auch in Polypen und in der normalen Schleimhaut in der Umgebung von Carcinomen nachgewiesen werden, womit die Befunde von Burtin et al. (1972a) bestätigt werden.
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Tappeiner, G., Denk, H., Eckerstorfer, R. et al. Vergleichende Untersuchungen über Auftreten und Lokalisation des carcinoembryonalen Antigens (CEA) und eines normalen perchlorsäureextrahierbaren Dickdarmschleimhaut-Antigens (NC) in Carcinomen und Polypen des Dickdarmes. Virchows Arch. Abt. A Path. Anat. 360, 129–140 (1973). https://doi.org/10.1007/BF00543224
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DOI: https://doi.org/10.1007/BF00543224