Advertisement

Rheumatology International

, Volume 2, Issue 2, pp 75–82 | Cite as

Characterisation of blood and synovial fluid lymphocytes from patients with rheumatoid arthritis and other joint diseases by monoclonal antibodies (OKT series) and acid α-naphthyl esterase staining

  • M. Duclos
  • H. Zeidler
  • W. Liman
  • W. J. Pichler
  • P. Rieber
  • H. H. Peter
Originals

Summary

Mononuclear cell preparations from peripheral blood (PBL) and synovial fluid (SFL) of 27 Patients with rheumatoid diseases (15 patients with definite rheumatoid arthritis (RA), 10 with other inflammatory joint diseases (OJD), 1 with sarcoid arthritis (SA) and 1 with traumatic arthritis (TA) were examined for lymphocyte subpopulations determined by monoclonal antibodies of the OKT series and by the dot-like, acid α-naphthyl esterase staining (ANAE) activity. In patients with classic, active RA, blood T cells carrying the OKT8+ (suppressor/killer) phenotype were significantly reduced leading to an elevated OKT4/OKT8 ratio of 4.1±0.4 compared with 2.1±0.1 in healthy controls.

In 10 patients with OJD this diminution of OKT8+ cells in peripheral blood was less pronounced or absent. As regards SFL subpopulations, patients with RA and OJD exhibited a similar distribution pattern with an elevation of OKT8+, Ia+ and ANAE negative cells and a similar OKT4/OKT8 ratio of 1.5±0.3 and 1.6±0.4, respectively. Similar results were also obtained in the only patient with TA, whereas the patient with SA and one RA patient with relapse after surgical synovectomy exhibited high OKT4/OKT8 ratios, both in synovial fluid and peripheral blood. Neither the OKT markers nor the dot-like ANAE staining pattern were significantly correlated to parameters of systemic or local disease activity as estimated by erythrocyte sedimentation rate and a local disease activity index.

Key words

Rheumatic diseases OKT markers ANAE staining Synovial fluid Blood 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Utsinger PD (1975) Synovial fluid lymphocytes in rheumatoid arthritis. Arthritis Rheum 18:595–602Google Scholar
  2. 2.
    Putte LBA van de, Meijer CJLM, Lafeber GJM, Kleinjan R, Cats A (1976) Lymphocytes in rheumatoid and non rheumatoid synovial fluids. Non-specificity of high T cell and low B cell percentages. Ann Rheum Dis 35:451–455Google Scholar
  3. 3.
    Burmester GR, Kalden JR, Peter HH, Schedel I, Beck P, Wittenborg A (1978) Immunological and functional characteristics of peripheral blood and synovial fluid lymphocytes from patients with rheumatoid arthritis. Scand J Immunol 7:405–417Google Scholar
  4. 4.
    Biberfeld G, Nilsson E, Biberfeld P (1979) T lymphocyte subpopulations in synovial fluid of patients with rheumatic disease. Arthritis Rheum 22:978–982Google Scholar
  5. 5.
    Mathieu A, Mereu MC, Pisano L (1981) Ty lymphocytes of peripheral blood and synovial fluid in rheumatoid arthritis. Arthritis Rheum 24:658–661Google Scholar
  6. 6.
    Reinherz EL, Schlossman SF (1980) The differentiation and function of human T lymphocytes. Cell 19:821–827Google Scholar
  7. 7.
    Reinherz EL, Schlossman SF (1980) Regulation of the immune response — inducer and suppressor T lymphocyte subsets in human beings. N Engl J Med 303:370–373Google Scholar
  8. 8.
    Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1979) Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci USA 76:4061–4065Google Scholar
  9. 9.
    Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1979) Further characterization of the human inducer T cell subset defined by monoclonal antibody. J Immunol 123:2894–2896Google Scholar
  10. 10.
    Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1980) A monoclonal antibody reactive with the human cytotoxic/suppressor cell subset previously defined by a heteroantiserum termed TH2. J Immunol 124:1301–1307Google Scholar
  11. 11.
    Lohmeyer J, Rieber P, Feucht H, Johnson J, Hadam M, Riethmüller G (1981) A subset of human NK cells isolated and characterized by monoclonal antibodies. Eur J Immunol 11:937–943Google Scholar
  12. 12.
    Manconi PE, Marrosu MG, Paghi C, Correale G, Zaccheo D (1979) Alpha-naphthyl acetate esterase activity in human lymphocytes: Distribution in lymphocyte subpopulation and in mitogen activated cells. Scand J Immunol 9:99Google Scholar
  13. 13.
    Pichler WJ, Lange ML, Birke Ch, Peter HH (1982) Relationship of Fc-IgG and Fc-IgM receptors to the antigens defined by OKT-antibodies and the acid α-naphthyl-acetate-esterase spot within human T cells. Immunobiology 160:424–437Google Scholar
  14. 14.
    Ropes MW, Bennett GA, Cobb S, Jacox R, Jessar R (1958) 1958 revision of diagnostic criteria for rheumatoid arthritis. Bull Rheum Dis 9:175–176Google Scholar
  15. 15.
    Kung PC, Goldstein G, Reinherz EL, Schlossman SF (1979) Monoclonal antibodies defining distinctive human T cell surface antigens. Science 206:347–349Google Scholar
  16. 16.
    Reinherz EL, Kung PC, Goldstein G, Leney RH, Schlossman SF (1980) Discrete stages of intrathymic differentiation: Analysis of monoclonal thymocytes and leukemic lymphoblasts of T cell lineage. Proc Natl Acad Sci USA 77:1588–1592Google Scholar
  17. 17.
    Reinherz EL, Kung PC, Pesando JM, Ritz J, Goldstein G, Schlossman SF (1979) Ia determinants on human T cell subsets defined by monoclonal antibody: Activation stimuli required for expression. J Exp Med 150:1472–1482Google Scholar
  18. 18.
    Breard J, Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1980) A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol 124:1943–1948Google Scholar
  19. 19.
    Rajvanshi V, Peter HH, Avenarius HJ (1979) Spontaneous cell mediated cytotoxicity (SCMC) associated with lymphocytes negative for acid α-naphthyl acetate esterase (ANAE) activity. Z Immunitätsforsch 155:330–337Google Scholar
  20. 20.
    Häntzschel H, Neumann HW (1978) Das synoviale System und seine diagnostische Bedeutung bei rheumatoider Arthritis und anderen Gelenkerkrankungen. Wiss Z Karl Marx Univ Leipzig Math Naturwiss Reihe 27:345Google Scholar
  21. 21.
    Morimoto C, Reinherz EL, Schlossman SF, Schur PH, Mills JA, Steinberg AD (1980) Alterations in immunoregulatory T cell subsets in active systemic lupus erythematoses. J Clin Invest 66:1171–1174Google Scholar
  22. 22.
    Reinherz EL (1981) T cell subsets in human disease — detection and pathophysiologic relevance. 9. Tagung des Arbeitskreises für klinische Immunologie, Frankfurt/Main-Hoechst, 13./14. November 1981 (communication)Google Scholar
  23. 23.
    Pfreundschuh M, Michel H, Parino G, Gram N, Stock G, Gause A, Hunstein W (1981) T lymphocyte subpopulations in RA. II. Definition by monoclonal antibodies. Z Rheumatol 40:245–249Google Scholar
  24. 24.
    Veys EM, Hermans P, Schnidler J, Kung PC, Goldstein G, Symoens S, Wauve J van (1982) Evaluation of T cell subsets with monoclonal antibodies in patients with rheumatoid arthritis. J Rheumatol (in press)Google Scholar
  25. 25.
    Burmester GR, Yu DTY, Irani AM, Kunkel HG, Winchester RJ (1981) Ia+T cells in synovial fluid and tissues of patients with rheumatoid arthritis. Arthritis Rheum 24:1370–1376Google Scholar
  26. 26.
    Hunninghake GW, Crystal RG (1981) Pulmonary sarcoidosis: A disorder mediated by excess helper T lymphocyte activity at sites of disease activity. N Engl J Med 305:429–434Google Scholar
  27. 27.
    Janossy G, Duke O, Poulter LW, Panayi G, Bofill M, Goldstein G (1981) Rheumatoid arthritis: A disease of T lymphocyte/macrophage immunoregulation. Lancet II:839–842Google Scholar
  28. 28.
    Janossy G, Tidman N, Papageorgiou ES, Kung PC, Goldstein G (1981) Distribution of T lymphocyte subsets in the human bone marrow and thymus: An analysis with monoclonal antibodies. J Immunol 126:1608–1613Google Scholar
  29. 29.
    Strelkauskas AS, Callery RT, McDowell J, Borel I, Schlossman SF (1978) Direct evidence for loss of human suppressor cells during active autoimmune disease. Proc Natl Acad Sci USA 75:5150–5154Google Scholar
  30. 30.
    Chattopadhyay C, Chattopadhyay H, Natvig JB, Michaelsen TE, Mellbye OJ (1979) Lack of suppressor cell activity in rheumatoid synovial lymphocytes. Scand J Immunol 10:309–316Google Scholar
  31. 31.
    Abdou NI, Lindsley HB, Racela LS, Pascual E, Hassanein KM (1981) Suppressor T cell dysfunction and anti-suppressor cell antibody in active early rheumatoid arthritis. J Rheumatol 8(1):9–18Google Scholar
  32. 32.
    Tosato G, Steinberg A, Blaese M (1981) Definitive EBV-specific suppressor T-cell function in rheumatoid arthritis. N Engl J Med 305(21):1238–1243Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • M. Duclos
    • 1
  • H. Zeidler
    • 2
  • W. Liman
    • 3
  • W. J. Pichler
    • 1
  • P. Rieber
    • 4
  • H. H. Peter
    • 1
  1. 1.Division of Clinical Immunology and Blood Transfusion, Internal Medicine CentreMedical School of HannoverHannoverFederal Republic of Germany
  2. 2.Department of Rheumatology, Internal Medicine CentreMedical School of HannoverHannoverFederal Republic of Germany
  3. 3.St. Josephs-StiftSendenhorst/WestfalenFederal Republic of Germany
  4. 4.Institute of ImmunologyUniversity of MunichMunichFederal Republic of Germany

Personalised recommendations