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Rheumatology International

, Volume 3, Issue 1, pp 1–5 | Cite as

Sjögren's syndrome in systemic lupus erythematosus and rheumatoid arthritis: Immune effector cells in salivary glands

  • Maria Malmström
  • Anu Seppä
  • Yrjö T. Konttinen
  • Tapani Helve
  • Sakari Reitamo
Originals

Summary

A simultaneously capturing azo dye method for acid α-naphthyl acetate esterase was used to characterize the cellular infiltrate in labial salivary glands in 25 patients with Sjögren's syndrome (SS). There was no significant difference in the T-pattern lymphocyte percentage in situ between the untreated group with SS and the group treated with 10±2 mg prednisone/day. There was a significant correlation (P<0.05) between the T-pattern lymphocyte percentage in situ and the focus-score value. In secondary (2°) SS in cases of systemic lupus erythematosus (SLE) and rheumatoid arthritis, respectively, 55%±4% (range 41–69) and 43%±7% (range 15–80) of all inflammatory cells in the periductal lymphocyte-rich infiltrates were T-pattern lymphocytes. In other SS patients the corresponding value was 28%±7% (range 4–50). The T-pattern lymphocyte percentage in situ was dependent on the disorder associated with SS (P=0.07). The present results indicate the dominance of T-lymphocytes in situ in 2° SS with SLE and suggest that there are differences in cell-mediated immunity in different clinical subgroups of SS.

Key words

Sjögren's syndrome Rheumatoid arthritis Systemic lupus erythematosus Salivary glands Lymphocytes 

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References

  1. 1.
    Strand V, Talal N (1980) Advances in the diagnosis and concept of Sjögren's syndrome (autoimmune exocrinopathy). Bull Rheum Dis 30:1046–1052Google Scholar
  2. 2.
    Pittsley RA, Daniels TE, Fye KH, Michalski JP, Talal N (1978) Autoimmune exocrinopathy: a new definition of Sjögren's syndrome confirmed by labial salivary gland biopsy. Arthritis Rheum 21:584Google Scholar
  3. 3.
    Nessan, VJ, Jacoway JR (1979) Biopsy of minor salivary glands in the diagnosis of sarcoidosis. N Engl J Med 301: 922–924Google Scholar
  4. 4.
    Talal N (1977) Autoimmunity and lymphoid malignancy: manifestations of immunoregulatory disequilibrium. In: Talal N (ed) Autoimmunity. Genetic, immunologic, virologic, and clinical aspects, chap 7. Academic Press, New York San Francisco LondonGoogle Scholar
  5. 5.
    Moutsopoulos HM, Chused TM, Mann DL, Klippel JH, Fauci AS, Frank MM, Lawley TJ, Hamburger MI (1980) Sjögren's syndrome (sicca syndrome): current issues. Ann Intern Med 92:212–226Google Scholar
  6. 6.
    Alarcón-Segovia D, Ibáñez G, Velázques-Forero F, Hernández-Ortiz J, González-Jiménez Y (1974) Sjögren's syndrome in systemic lupus erythematosus. Clinical and subclinical manifestations. Ann Intern Med 81:577–583Google Scholar
  7. 7.
    Moutsopoulos HM, Klippel JH, Pavlidis N, Wolf RO, Sweet JB, Steinberg AD, Chu FC, Tarpley TM (1980) Correlative histologic and serologic findings of sicca syndrome in patients with systemic lupus erythematosus. Arthritis Rheum 23: 36–40Google Scholar
  8. 8.
    Cohen AS, Reynolds WE, Franklin EC, Kulka JP, Ropes MW, Shulman LE, Wallace SL (1971) Preliminary criteria for the classification of systemic lupus erythematosus. Bull Rheum Dis 21:643–648Google Scholar
  9. 9.
    Ropes MW, Bennett GA, Cobb J, Jacox R, Jessar RA (1958) 1958 revision of diagnostic criteria for rheumatoid arthritis. Bull Rheum Dis 9:175–176Google Scholar
  10. 10.
    Ranki A, Reitamo S, Konttinen YT, Häyry P (1980) Histochemical identification of human T lymphocytes from paraffin sections. J Histochem Cytochem 28:704–707Google Scholar
  11. 11.
    Horwitz DA, Allison AC, Ward P, Kight N (1977) Identification of human mononuclear leucocyte populations by esterase staining. Clin Exp Immunol 30:289–298Google Scholar
  12. 12.
    Ranki A, Tötterman TH, Häyry P (1976) Identification of resting human T and B lymphocytes with acid α-naphthyl acetate esterase staining combined to rosette formation with staphylococcus aureus strain cowan I. Scand J Immunol 5:1129–1138Google Scholar
  13. 13.
    Kulenkampff J, Janossy G, Greaves MF (1977) Acid esterase in human lymphoid cells and leukemic blasts: a marker for T lymphocytes. Br J Haematol 36:231–240Google Scholar
  14. 14.
    Knowles DM II, Hoffman T, Ferrarini M, Kunkel HG (1978) The demonstration of acid α-naphthyl acetate esterase activity in human lymphocytes: usefulness as a T-cell marker. Cell Immunol 35:112–123Google Scholar
  15. 15.
    Konttinen YT, Reitamo S, Ranki A, Häyry P, Kankaanpää U, Wegelius O (1981) Characterization of the immunocompetent cells of rheumatoid synovium from tissue sections and eluates. Arthritis Rheum 24:71–79Google Scholar
  16. 16.
    Willson JKV Jr, Zaremba JL, Pitts AM, Pretlow TG II (1976) A characterization of human tonsillar lymphocytes after separation from other tonsillar cells in an isokinetic gradient of Ficoll in tissue culture medium. Am J Pathol 83:341–355Google Scholar
  17. 17.
    Brandzaeg P, Surjan L Jr, Berdal P (1978) Immunoglobulin systems of human tonsils. I. Control subjects of various ages: quantification of Ig-producing cells, tonsillar morphometry and serum Ig concentrations. Clin Exp Immunol 31:367–381Google Scholar
  18. 18.
    McConnell I, Munro A, Waldmann H (1981) The immune system. A course on the molecular and cellular basis of immunity, 2nd edn, chap 14. Blackwell, Oxford London Edinburgh Boston MelbourneGoogle Scholar
  19. 19.
    Chused TM, Hardin JA, Frank MM, Green I (1974) Identification of cells infiltrating the minor salivary glands in patients with Sjögren's syndrome. J Immunol 112:641–648Google Scholar
  20. 20.
    Koriyama K, Arimori S (1978) The distribution of lymphocyte subpopulations in human tissue. Part II. Studies on detection of lymphocyte subpopulations infiltrating in the target organs of various autoimmune disorders. Acta Haematol Jpn 41:72–80Google Scholar
  21. 21.
    Talal N, Sylvester RA, Daniels TE, Greenspan JS, Williams RC Jr (1974) T and B lymphocytes in peripheral blood and tissue lesions in Sjögren's syndrome. J Clin Invest 53:180–189Google Scholar
  22. 22.
    Konttinen YT (1981) In situ characterization of the cellular infiltrate in labial and palatine glands in Sjögren's syndrome. Scand J Rheumatol 10:321–330Google Scholar
  23. 23.
    Tannenbaum H, Pinkus GS, Anderson LG, Schur PH (1975) Immunologic characterization of the mononuclear cell infiltrates in rheumatoid synovia, in rheumatoid nodules, and in lip biopsies from patients with Sjögren's syndrome. Arthritis Rheum 18:305–314Google Scholar
  24. 24.
    Fox RI, Carstens SA, Fong S, Robinson CA, Howell F, Vaughan JH (1982) Use of monoclonal antibodies to analyze peripheral blood and salivary gland lymphocyte subsets in Sjögren's syndrome. Arthritis Rheum 25:419–426Google Scholar
  25. 25.
    Greenspan JS, Gutman GA, Weissman IL, Talal N (1974) Thymus-antigen- and immunoglobulin-positive lymphocytes in tissue infiltrates of NZB/NZW mice. Clin Immunol Immunopathol 3:16–31Google Scholar
  26. 26.
    Ramos-Niembro F, Alarcón-Segovia D (1979) Development of sicca symptoms in systemic lupus erythematosus patients with existing subclinical abnormalities of lacrimal and/or salivary glands. Arthritis Rheum 22:935–936Google Scholar
  27. 27.
    Zvaifler NJ (1981) Etiology and pathogenesis of systemic lupus erythematosus. In: Kelley WN, Harris ED Jr, Ruddy S, Sledge CP (eds) Textbook of rheumatology, chap 68. Saunders, Philadelphia London TorontoGoogle Scholar
  28. 28.
    Raveche ES, Steinberg AD (1979) Lymphocytes and lymphocyte functions in systemic lupus erythematosus. Semin Hematol 16:344–370Google Scholar
  29. 29.
    Konttinen YT, Tolvanen E, Johansson E, Reitamo S, Wegelius O (1982) Skin response to intradermal deoxyribonucleic and ribonucleic acids in systemic lupus erythematosus. Arthritis Rheum (in press)Google Scholar
  30. 30.
    Schmitt D, Alario A, Thivolet J (1979) In situ characterization of tissue lymphoid cells of cutaneous infiltrates using specific membrane antigens. Clin Exp Dermatol 4:161–173Google Scholar
  31. 31.
    Konttinen YT, Reitamo S, Ranki A, Segerberg-Konttinen M (1981) T lymphocytes and mononuclear phagocytes in the skin infiltrate of systemic and discoid lupus erythematosus and Jessner's lymphocytic infiltrate. Br J Dermatol 104: 141–145Google Scholar
  32. 32.
    Nasonova V, Vasiljev V (1981) Sjögren-Syndrom und Sjögren-Krankheit in der Rheumatologie. Z Gesamte Inn Med 36: 199–203Google Scholar
  33. 33.
    Anderson LG, Cummings NA, Asofsky R, Hylton MB, Tarpley TM Jr, Tomasi TB Jr, Wolf RO, Schall GL, Talal N (1972) Salivary gland immunoglobulin and rheumatoid factor synthesis in Sjögren's syndrome. Natural history and response to treatment. Am J Med 53:456–463Google Scholar
  34. 34.
    Gratwhol AA, Moutsopoulos HM, Chused TM, Akizuki M, Wolf RO, Sweet JB, Deisseroth AB (1977) Sjögren-type syndrome after allogeneic bone-marrow transplantation. Ann Intern Med 87:703–706Google Scholar
  35. 35.
    Moutsopoulos HM, Fauci AS (1980) Immunoregulation in Sjögren's syndrome. Influence of serum factors on T-cell subpopulations. J Clin Invest 65:519–528Google Scholar
  36. 36.
    Morimoto C, Reinherz EL, Nadler LM, Distaso JA, Steinberg AD, Schlossman SF (1982) Comparison in T- and B-cell markers in patients with Sjögren's syndrome and systemic lupus erythematosus. Clin Immunol Immunopathol 22: 270–278Google Scholar
  37. 37.
    Berry H, Bacon PA, Davis JR (1972) Cell-mediated immunity in Sjögren's syndrome. Ann Rheum Dis 31:298–302Google Scholar
  38. 38.
    Miyasaka N, Sauvezie B, Pierce DA, Daniels TE, Talal N (1980) Decreased autologous mixed lymphocyte reaction in Sjögren's syndrome. J Clin Invest 66:928–933Google Scholar
  39. 39.
    Minato N, Takeda A, Kano S, Takaku F (1982) Studies of the functions of natural killer-interferon system in patients with Sjögren's syndrome. J Clin Invest 69:581–588Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Maria Malmström
    • 1
  • Anu Seppä
    • 1
    • 2
  • Yrjö T. Konttinen
    • 1
  • Tapani Helve
    • 1
  • Sakari Reitamo
    • 3
  1. 1.Institute of Dentistry, Department of Oral SurgeryUniversity of HelsinkiHelsinki 28Finland
  2. 2.Fourth Department of MedicineUniversity of HelsinkiHelsinki 17Finland
  3. 3.College of Physicians and Surgeons, Institute of Cancer ResearchColumbia UniversityNew YorkUSA

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