Rheumatology International

, Volume 4, Issue 2, pp 49–53 | Cite as

In vitro synthesis of IgM and IgM rheumatoid factor in seronegative arthritides

  • G. S. Alarcón
  • W. J. Koopman
  • R. E. Schrohenloher


In vitro patterns of IgM and IgM rheumatoid factor (RF) synthesis exhibited by peripheral blood B cells (MNL) obtained from healthy individuals as well as patients with seropositive and seronegative rheumatoid arthritis (RA) have been previously examined. The present study was performed in a group of patients with non-rheumatoid seronegative arthritis (SNA) in order to compare patterns of in vitro IgM and IgM RF synthesis to that previously observed with seropositive and seronegative RA. Eighteen patients with SNA (4 ankylosing spondylitis, 10 psoriatic arthritis, and 4 unclassified variant disease) were studied as well as 18 healthy adult controls.

Spontaneous release of IgM RF by MNL was not observed in SNA or controls. In contrast, IgM RF was detected in pokeweed mitogen (PWM)-stimulated MNL culture supernatants from 9/18 SNA (mean±SD=17.0±9.9 ng/106 cells), and 10/18 normal controls (16.7±9.9 ng). Synthesis of IgM by PWM-stimulated MNL from SNA (2 062±1 200 ng) was significantly less than observed with MNL from controls (4 093±1 896 ng) (P<0.001). There were no differences among the various SNA subsets with regards to the levels of IgM and IgM RF produced either spontaneously or after PWM stimulation. IgM RF constituted a small fraction of the total IgM in SNA and normals (0.9% and 0.5%, respectively). This is clearly distinct from seropositive RA in which we have previously established that IgM RF constitutes a substantial fraction of the total IgM (10.7%) (P<0.01). IgM and IgM RF production did not appear to be significantly influenced by immunosuppressive medication.

The results suggest that regulatory mechanisms governing the expression of RF are intact in patients with SNA and that lack of in vivo RF production in these patients cannot be related to an absolute deficiency of PWM-inducible mature RF committed B cells.

Key words

Seronegative arthropathies Rheumatoid factor production/regulation 


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  1. 1.
    Carson DA (1981) Rheumatoid factor. In: Kelley WN, Harris ED, Ruddy S, Sledge CB (eds) Textbook of rheumatology. Saunders, Philadelphia London Toronto, pp 677–690Google Scholar
  2. 2.
    Koopman WJ, Schrohenloher RE (1983) Rheumatoid factor. In: Utsinger PD, Zvaifler NJ, Ehrlich GE (eds) Primary care of rheumatoid arthritis. Lippincott, Philadelphia (in press)Google Scholar
  3. 3.
    Vaughan JH, Chihara T, Moore TL, Robbins DL, Tanimoto K, Johnson JS, McMillan R (1976) Rheumatoid factor-producing cells detected by direct hemolytic plaque assay. J Clin Invest 58:933–941Google Scholar
  4. 4.
    Slaughter L, Carson DA, Jensen FC, Holbrook T, Vaughan JH (1978) In vitro effects of Epstein-Barr virus on peripheral blood mononuclear cells from patients with rheumatoid arthritis and normal subjects. J Exp Med 148:1429–1434Google Scholar
  5. 5.
    Koopman WJ, Schrohenloher RE (1980) In vitro synthesis of IgM rheumatoid factor by lymphocytes from healthy adults. J Immunol 125:934–939Google Scholar
  6. 6.
    Koopman WJ, Schrohenloher RE (1980) Enhanced in vitro synthesis of IgM rheumatoid factor in rheumatoid arthritis. Arthritis Rheum 23:985–992Google Scholar
  7. 7.
    Pasquali JL, Tsoukas CD, Hench PK, Vaughan JH, Carson DA (1981) Selective lymphocyte deficiency in seronegative rheumatoid arthritis. Arthritis Rheum 24:770–773Google Scholar
  8. 8.
    Alarcón GS, Koopman WJ, Schrohenloher RE (1982) Differential patterns of in vitro IgM rheumatoid factor synthesis in seronegative and seropositive rheumatoid arthritis. Arthritis Rheum 25:150–155Google Scholar
  9. 9.
    Alarcón GS, Koopman WJ, Acton RT, Barger BO (1982) Seronegative rheumatoid arthritis. A distinct immunogenetic disease. Arthritis Rheum 25:502–507Google Scholar
  10. 10.
    Stastny P (1980) Rheumatoid arthritis. Joint report from the 8th Histocompatibility Workshop, Los AngelesGoogle Scholar
  11. 11.
    Doubloug JH, Førre Ø, Kass E, Thorsby E (1980) HLA-antigens and rheumatoid arthritis: association between HLA-DRw4 positivity and IgM rheumatoid factor production. Arthritis Rheum 23:309–313Google Scholar
  12. 12.
    Swiss Federal Commission for the Rheumatic Diseases, Subcommission for Research (1981) HLA-DR antigens in rheumatoid arthritis. A Swiss collaborative study. Rheumatol Int 1:111–113Google Scholar
  13. 13.
    Masi AT, Feigenbaum SL (1983) Seronegative rheumatoid arthritis (SNRA): fact or fiction. Arch Intern Med 143:2167–2172Google Scholar
  14. 14.
    Moll JMH, Haslock I, Macvae MB, Wright V (1974) Association between ankylosing spondylitis, psoriatic arthritis, Reiter's disease, the intestinal arthropathies and Behcet's syndrome. Medicine 53:343–346Google Scholar
  15. 15.
    Kellgren JH, Jeffrey MR, Ball J (1963) The epidemiology of chronic rheumatism, vol 1. Blackwell, Oxford, p 326Google Scholar
  16. 16.
    Bennett PH, Wood PHN (1968) Population studies of the rheumatic diseases. Amsterdam, Excerpta Medica, p 456Google Scholar
  17. 17.
    Böyum A (1968) Separation of leukocytes from blood and bone marrow. Scand J Clin Lab Invest 21 (Suppl 97):77–82Google Scholar
  18. 18.
    Koopman WJ, Schrohenloher RE (1980) A sensitive radioimmunoassay for quantification of IgM rheumatoid factor. Arthritis Rheum 23:302–308Google Scholar
  19. 19.
    Poikonen K, Oka M, Mottonen T, Jokinen I, Arvilommi H (1982) Synthesis of IgM, IgG and IgA in rheumatoid arthritis. Ann Rheum Dis 41:607–611Google Scholar
  20. 20.
    Room GR, Plater-Zyberk C, Clarke MF, Maini RN (1982) B-lymphocyte subpopulation which forms rosettes with mouse erythrocytes increased in rheumatoid arthritis. Rheumatol Int 2:175–178Google Scholar
  21. 21.
    Lucivero G, Lawton AR, Cooper MD (1981) Rosette formation with mouse erythrocytes defines a population of human B lymphocytes unresponsive to pokeweed mitogen. Clin Exp Immunol 45:185–192Google Scholar
  22. 22.
    Fong S, Vaughan JH, Carson DA (1983) Two different rheumatoid factor-producing cell populations distinguished by the mouse erythrocyte receptor and responsiveness to polyclonal B cell activators. J Immunol 130:162–164Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • G. S. Alarcón
    • 1
  • W. J. Koopman
    • 1
  • R. E. Schrohenloher
    • 1
  1. 1.Division of Clinical Immunology and Rheumatology, Department of MedicineUniversity of Alabama in Birmingham, and Birmingham Veterans Administration HospitalBirminghamUSA

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