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Rheumatology International

, Volume 3, Issue 3, pp 133–138 | Cite as

The effect of rheumatoid synovial fluid macrophages on DNA, glycosaminoglycan and collagen synthesis by synovial fibroblasts

  • K. Pulkki
  • E. Vuorio
  • S. Jalava
Originals

Summary

The effects of soluble factors secreted by peripheral blood monocytes and rheumatoid synovial fluid macrophages were tested on human synovial fibroblast cultures. Both monocytes and macrophages liberated factors which reduced DNA synthesis (3H-thymidine incorporation) by synovial fibroblasts. Monocyte and macrophage factors stimulated hyaluronic acid synthesis. The activation obtained with rheumatoid synovial macrophages was considerably greater than that with monocytes. Foetal bovine serum was found to have a clear stimulatory effect on the synthesis of collagen and other proteins by fibroblasts. The effects of monocyte and macrophage factors on protein synthesis in synovial fibroblasts were small: collagen synthesis was slightly increased relative to other extracellular proteins.

Key words

Monocyte Macrophage Fibroblast Collagen Hyaluronic acid 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • K. Pulkki
    • 1
  • E. Vuorio
    • 1
  • S. Jalava
    • 2
  1. 1.Department of Medical ChemistryUniversity of TurkuTurku 52Finland
  2. 2.Department of Medicine (Paimio Hospital)University Central Hospital of TurkuTurku 52Finland

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