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Identification and purification of a 55K polypeptide in Sjögren's syndrome A antigen

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Summary

The Sjögren's syndrome A antigen (SS-A) purified by affinity chromatography from human spleen was shown to contain a major polypeptide with a mol. wt. of 55 000 daltons confirmed as SS-A by its reactivity with different anti-SS-A sera on affinity columns, immunodiffusion and the Western blot. A similar reactive polypeptide was demonstrated in extracts of rabbit liver, brain, spleen, kidney, lung and thymus.

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Authors and Affiliations

  1. Kennedy Institute of Rheumatology, 6 Bute Gardens, W6 7DW, London, UK

    P. R. Smith, P. J. W. Venables, D. Williams & R. N. Maini

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  1. P. R. Smith
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  2. P. J. W. Venables
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  3. D. Williams
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  4. R. N. Maini
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Smith, P.R., Venables, P.J.W., Williams, D. et al. Identification and purification of a 55K polypeptide in Sjögren's syndrome A antigen. Rheumatol Int 4, 135–138 (1984). https://doi.org/10.1007/BF00541183

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  • DOI: https://doi.org/10.1007/BF00541183

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