Summary
The localisation of fibronectin in the synovial membrane of rheumatoid arthritis (RA) and other chronic inflammatory joint diseases has been studied using a peroxidase-antiperoxidase immunohistochemical method. Synovia were studied from seven cases of seropositive RA three cases of seronegative RA, six cases of ankylosing spondylitis, four cases of Reiter's syndrome and five of psoriatic arthritis. Six were small biopsies and the remaining tissues were obtained at open surgery for orthopaedic procedures or biopsies.
Fibronectin was demonstrated in all of the synovia examined and was present in intimal cells, synovial giant cells, the walls of small blood vessels, basement membrane of larger vessels and deposits of fibrin. No difference in this distribution of fibronectin was found in seropositive and seronegative RA, ankylosing spondylitis, Reiter's syndrome or psoriatic arthritis, neither was there any difference in the amount of fibronectin at various sites.
Similar content being viewed by others
References
Mosesson MW, Amrani DL (1980) The structure and biologic activities of plasma fibronectin. Blood 56:145–158
Scott DL, Delamere JP, Walton KW (1981) The distribution of fibronectin in the pannus in rheumatoid arthritis. Br J Exp Pathol 62:362–368
Scott DL, Wainwright AC, Walton KW, Williamson N (1981) Significance of fibronectin in rheumatoid arthritis and osteoarthrosis. Ann Rheum Dis 40:142–153
Vartio T, Vaheri A, Essen R von, Isomäki H, Stenman S (1981) Fibronectin in synovial fluid and tissue in rheumatoid arthritis. Eur J Clin Invest 11:207–212
Hardy PH, Petrali JP, Sternberger LA (1970) Postembedding staining for electron microscopy by the unlabelled antibody enzyme method. J Histochem Cytochem 18:678 (Abstr)
Taylor CR (1974) The nature of Reed-Sternberg cells and other malignant ‘reticulum’ cells. Lancet II:802–806
Burns J (1975) Background staining and the sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material. Histochemistry 43:291–294
Sternberger LA (1979) Immunocytochemistry, 2nd edn. Wiley, New York
Stenman S, Vaheri A (1978) Distribution of a major connective tissue protein, fibronectin, in normal human tissues. J Exp Med 147:1054–1064
Linder E, Stenman S, Lehto V-P, Vaheri A (1978) Distribution of fibronectin in human tissue and relationship to other connective tissue components. Ann NY Acad Sci 312:151–159
Fyrand O (1979) Studies of fibronectin in the skin. I Indirect immunofluorescence studies on normal human skin. Br J Dermatol 101:263–270
Cooper SM, Keyser AJ, Beaulieu AD, Ruoslahati E, Nimi ME, Quismori FP (1979) Increase in fibronectin in the deep dermis of involved skin in progressive systemic sclerosis. Arthritis Rheum 22:983–987
Pettersson EE, Colvi RB (1978) Cold insoluble globulin (fibronectin, L.E.T.S. protein) in normal and diseased human glomeruli. Clin Immunol Immunopathol 11:425–436
Weiss WA, Boon SO, Yeut M, Engrall E, Ruoslahati E (1979) Immunofluorescent localisation of fibronectin in the human kidney. Lab Invest 41:340–347
Hahn E, Wick G, Pencev D, Timpl R (1980) Distribution of basement membrane proteins in normal and fibrotic human liver: collagen type IV, Laminin and fibronectin. Gut 21:63–71
Couchman JR, Gibson WT, Them D, Weaver AC, Rees DA, Parish WE (1979) Fibronectin distribution in epithelial and associated tissues of the rat. Arch Dermatol Res 266:295–310
Burns J, Dixon AJ, Woods JC (1980) Immunoperoxidase localisation of fibronectin in glomeruli of formalin fixed paraffin processed renal tissue. Histochemistry 67:73–78
Dixon AJ, Burns J, Dunnill MS, McGee JO'D (1980) Distribution of fibronectin in normal and diseased human kidneys. J Clin Pathol 33:1021–1028
Hølund B (1981) The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue. Histochemistry 72:291–299
Huang SN, Minassian M, More JD (1976) Application of immunofluorescent staining on paraffin sections improved by trypsin digestion. Lab Invest 35:383–390
Curran RC, Gregory J (1978) Demonstration of immunoglbulins in cryostal and paraffin sections of human tonsil by immunofluorescence and immunoperoxidase techniques. Effects of processing on immunohistochemical performance of tissues and on the use of proteolytic enzymes to unmask antigens in sections. J Clin Pathol 31:974–983
Hautzner NW, Wittkuhn JF, McCaughey WTE (1980) Trypsin digestion in immunoperoxidase staining. J Histochem Cytochem 28:52–53
Mempham BL, Frater W, Mitchell BS (1979) The use of proteolytic enzymes to improve the immunoglobulin staining by the PAP technique. Histochem J 11:345–357
Hølund B, Clemmensen I (1982) The value of hyaluronidase treatment of different tissues before demonstration of fibronectin by the indirect immunoperoxidase technique. Histochemistry 76:517–525
Clemmenson I (1981) Fibronectin and its role in connective tissue diseases. Eur J Clin Invest 11:145–146
Rennard SI, Hunninghake GW, Bitterman PB, Crystal RG (1981) Production of fibronectin by the human alveolar macrophage: mechanism for the recruitment of fibroblasts to sites of tissue injury in interstitial lung disease. Proc Natl Acad Sci USA 78:7147–7151
Yamada KM, Akiyama SK, Hayashi M (1970) Fibronectin structure and function, and its interactions with glycosaminoglycans. Biochem Soc Trans 9:506–508
Yamada KM, Olden K (1978) Fibronectins — adhesive glycoproteins of cell surface and blood. Nature 275:179–184
Kurkinen M, Vaheri A, Roberts PJ, Stenman S (1980) Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest 43:47–51
Repesh LA, Fitzgerald TJ, Furcht LT (1982) Fibronectin involvement in granulation tissue and wound healing in rabbits. J Histochem Cytochem 30:351–358
Saba TM, Blumenstock FA, Weber P, Kaplan JE (1978) Physiologic role of cold insoluble globulin in systemic host defense: Implications of its characterisation as the opsonic α2-surface-binding glycoprotein. Ann NY Acad Sci 312:43–55
Hopper KE, Adelmann BC, Gentner G, Gay S (1976) Recognition by guinea-pig peritoneal exudate cells of conformationally different states of the collagen molecule. Immunology 30:249–259
McDonald JA, Kelley DG (1980) Degradation of fibronectin by human leucocyte elastase. J Biol Chem 255:8848–8858
Scott DL, Walton KW, Hunneyball IM (1983) Fibronectin in experimental antigen-induced arthritis. J Pathol 140:177 (Abstr)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mayston, V., Mapp, P.I., Davies, P.G. et al. Fibronectin in the synovium of chronic inflammatory joint disease. Rheumatol Int 4, 129–133 (1984). https://doi.org/10.1007/BF00541182
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00541182