Internationales Archiv für Arbeitsmedizin

, Volume 29, Issue 2, pp 124–128 | Cite as

The levels of the nicotinamide-adenine dinucleotides in the tissues of rabbits and rats after chronic exposure to carbon disulphide

  • Teresa Wrońska-Nofer
  • Jerzy A. Sokal


The levels of nicotinamide-adenine dinucleotides in blood and liver of rats and rabbits chronically intoxicated with carbon disulphide were studied.

In both species of animals exposure to CS2 brought about a decrease in the total amount of blood nucleotides and caused an increase of NADPH concentration in the liver.

A possible correlation of observed changes in the levels of nucleotides in liver with disorders in lipid metabolism arising in the course of chronic exposure to CS2 is discussed.


Public Health Lipid Nucleotide NADPH Lipid Metabolism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Abraham, S., Matthes, K. J., Chaikoff, I. L.: Fatty acid synthesis from acetate by normal and diabetic rat liver homogenate fractions. I. A comparison of cofactor requirements. J. biol. Chem. 235, 2551–2559 (1960).Google Scholar
  2. 2.
    Brady, R. O., Bradley, R. M., Trams, E. G.: Biosynthesis of fatty acids. I. Studies with enzymes obtained from liver. J. biol. Chem. 235, 3093–3098 (1960).Google Scholar
  3. 3.
    Christie, G. S., Le Page, R. N.: Liver damage in acute heliotrine poisoning. I. The intracellular distribution of pyridine nucleotides. Biochem. J. 84, 25–38 (1962).Google Scholar
  4. 4.
    Donaldson, W. E., Witt-Peeters, E. M., Scholte, H. R.: Fatty acid synthesis in rat liver: relative contributions of the mitochondrial, microsomal and non-particulate systems. Biochim. biophys. Acta (Amst.) 202, 35–42 (1970).Google Scholar
  5. 5.
    Foster, D. W., Bloom, D.: A comparative study of reduced di- and triphosphopyridine nucleotides in the intact cell. J. biol. Chem. 236, 2548–2551 (1961).Google Scholar
  6. 6.
    Glock, G. E., McLean, P.: The intracellular distribution of pyridine nucleotides in rat liver. Exp. Cell Res. 11, 234–236 (1956).Google Scholar
  7. 7.
    Jacobson, K. B., Kaplan, N. O.: Pyridine coenzymes of subcellular tissue fractions. J. biol. Chem. 226, 603–613 (1957).Google Scholar
  8. 8.
    Knauss, H. J., Porter, J. W., Wasson, G.: The biosynthesis of mevalonic acid from 1-C14-acetate by a rat liver enzyme system. J. biol. Chem. 234, 2835–2840 (1959).Google Scholar
  9. 9.
    Kunz, W., Schaude, G., Sehimassek, H., Schmid, W., Siess, M.: Stimulation of liver growth by drugs. Proceedings of the European society for the Study of Drug Toxicity, Meeting in Rome 1966, Vol. 7, pp. 138–153. Amsterdam: Excerpta Medica Foundation 1966.Google Scholar
  10. 10.
    Lagunas, R., McLean, P., Greenbaum, A. L.: The effect of raising the NAD+ content on the pathways of carbohydrate metabolism and lipogenesis in the rat liver. Europ. J. Biochem. 15, 179–190 (1970).Google Scholar
  11. 11.
    Langdon, R. G.: The biosynthesis of fatty acids in rat liver. J. biol. Chem. 226, 615–629 (1957).Google Scholar
  12. 12.
    Marinotti, G. V.: Biosynthesis of triglyerides. In: Comprehensive biochemistry, Eds. M. Florkin, E. H. Stotz, Vol. 18, pp. 117–155. Amsterdam-London-New York: Elsevier Publishing Company 1970.Google Scholar
  13. 13.
    Nofer, J., Wrońska, T.: Elimination des certains metabolites de niacine par rapport au temps d'exposition et la dose de sulfure de carbone. Intern. Congr. Occupat. Health, Vienna 1966, pp. 355–358. Wien: Wien. Med. Acad. 1966.Google Scholar
  14. 14.
    Siperstein, M. D., Fagan, V. M.: Feedback control of mevalonate synthesis by dietary cholesterol. J. biol. Chem. 241, 602–609 (1966).Google Scholar
  15. 15.
    Sokal, J. A., Tarkowski, S., Wrońska-Nofer, T.: A simplified method for determination of nicotinamide-adenine dinucleotides in liver tissue. Acta biochim. pol. 16, 1–10 (1969).Google Scholar
  16. 16.
    Sokal, J. A., Wrońska-Nofer, T.: Assays of pyridine nucleotides in blood of human subjects and of experimental animals. Diagnostyka Laboratoryjna 5, 299–304 (1969).Google Scholar
  17. 17.
    White, H. B., Mitsuhashi, O., Bloch, K.: Pyridine nucleotides requirements of fatty acid synthetases. J. biol. Chem. 246, 4751–4754 (1971).Google Scholar
  18. 18.
    Witting, L., Porter, J. W.: Intermediates in the conversion of mevalonic acid to squalene by a rat liver enzyme system. J. biol. Chem. 234, 2841–2846 (1959).Google Scholar
  19. 19.
    Wrońska-Nofer, T.: Effect of nicotinic acid on lipids level in the serum of rats intoxicated with carbon disulphide. Med. Pracy 19, 426–434 (1968).Google Scholar
  20. 20.
    Wrońska-Nofer, T.: The incorporation of 14C-acetate into cholesterol in rats exposed to carbon disulphide. Biochem. Pharmacol. 18, 925–926 (1969).Google Scholar
  21. 21.
    Wrońska-Nofer, T.: The influence of nicotinic acid upon the disturbances in the lipid metabolism caused by carbon disulphide in rats. Int. Arch. Arbeitsmed. 27, 221–227 (1970).Google Scholar
  22. 22.
    Wrónska-Nofer, T., Nofer, J., Tarkowski, S.: Excretion of niacin metabolites in animals intoxicated with carbon disulphide. Med. Pracy 16, 77–81 (1965).Google Scholar
  23. 23.
    Wrońska-Nofer, T., Sokal, J. A., Tarkowski, S.: The level of nicotinamide adenine nucleotides in the tissues of rat in the course of chronic carbon disulphide intoxication. Med. Pracy 21, 249–252 (1970).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Teresa Wrońska-Nofer
    • 1
  • Jerzy A. Sokal
    • 1
  1. 1.Department of BiochemistryInstitute of Occupational MedicinełódźPoland

Personalised recommendations