Internationales Archiv für Arbeitsmedizin

, Volume 29, Issue 2, pp 119–123 | Cite as

Effect of protein malnutrition on cholesterol and phosphatases in the lungs of silicotic rats

  • G. S. D. Gupta
  • J. L. Kaw
  • S. H. Zaidi


The role of dietary proteins on total cholesterol and its fractions and phosphatases was studied in short term experiments in the lungs of rats following exposure to silica dust by intratracheal inoculation of saline suspension. The animals were fed either a diet complete in all respects or a diet grossly deficient in proteins but otherwise adequate. Feeding of a diet low or adequate in protein did not influence the increased cholesterol content of the lungs of silicotic animals or the levels of lung acid and alkaline phosphatases.


Public Health Cholesterol Dust Alkaline Phosphatase Total Cholesterol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allison, A. C., Harington, J. S., Birbeck, M.: An examination of the cytotoxic effects of silica on macrophages. J. exp. Med. 124, 141–153 (1966).Google Scholar
  2. Bargoni, N.: Enzymatic activity and protein amounts in the liver. Experientia (Basel) 7, 104–105 (1951).Google Scholar
  3. Bodansky, A.: Phosphatase studies. I. Determination of inorganic phosphate. Beer's law and interfering substances in the Kuttnerlichtenstein method. J. biol. Chem. 99, 197–206 (1932).Google Scholar
  4. Cohen, A. M., Teitelbaum, A.: Effect of different levels of protein in sucrose and starch diets on lipid synthesis in the rat. Israel J. med. Sci. 2, 727–732 (1966).Google Scholar
  5. Engelbrecht, F. M., Paul, R.: Enzymes in the development of silicotic fibrosis in the lungs of rats. Ann. occup. Hyg. 1, 135–148 (1959).Google Scholar
  6. Fiske, C. H., Subbarow, Y.: The colorimetric determination of phosphorous. J. biol. Chem. 66, 375–400 (1925).Google Scholar
  7. Kaw, J. L., Gupta, G. S. D., Zaidi, S. H.: Lung lipids and pulmonary silicosis in rats. Int. Arch. Arbeitsmed. 27, 324–330 (1971).Google Scholar
  8. Marks, G. S., Marasas, L. W.: Changes in the lung lipids of rabbits and guinea pigs exposed to the inhalation of silica dust. Brit. J. industr. Med. 17, 31–35 (1960).Google Scholar
  9. Mukherjee, K. L., Sarkar, N. K.: Liver enzymes in human undernutrition. Brit. J. Nutr. 12, 1–7 (1958).Google Scholar
  10. Nadler, S., Goldfischer, S.: The intracellular release of lysosomal contents in macrophages that have ingested silica. J. Histochem. Cytochem. 18, 368–371 (1970).Google Scholar
  11. Nishida, T., Takenaka, F., Kummerow, F. A.: Effect of dietary protein and heated fat on serum cholesterol and beta-lipoprotein levels, and on the incidence of experimental atherosclerosis in chicks. Circulat. Res. 6, 194–202 (1958).Google Scholar
  12. Pernis, B.: Biochemical studies on the hyaline tissue of silicosis. I. Fibrous proteins content (collagen and elastin). Med. d. Lavoro 46, 659–667 (1955).Google Scholar
  13. Rilke, F., Pernis, B.: Morphological and histochemical aspects of RES stimulation induced by crystalline silica particles. Med. d. Lavoro 57, 321–330 (1961).Google Scholar
  14. Seemann, G.: über das Schicksal des ins Blut eingeführten Cholesterin, insbesondere über die Filtration und AbwehrvorgÄnge im Lungengewebe. Beitr. path. Anat. 83, 705–718 (1930).Google Scholar
  15. Shinowara, G. Y., Johnes, L. M., Reinhart, H. L.: The estimation of serum inorganic phosphate and “acid” and “alkaline” phosphatase activity. J. biol. Chem. 142. 921–933 (1942).Google Scholar
  16. Williams, J. N., Jr.: Response of the liver to prolonged protein depletion. V. Neutral glycerides and cholesterol. Production of fatty livers by certain amino acids fed in a protein-free ration. J. Nutr. 85, 73–81 (1965).Google Scholar
  17. Yeh, Yu-Yan, Leveille, G. A.: Effect of dietary protein on hepatic lipogenesis in the growing chick. J. Nutr. 98, 356–366 (1969).Google Scholar
  18. Zaidi, S. H.: Experimental pneumoconiosis, p. 48. Baltimore, Maryland: John Hopkins Press 1969.Google Scholar
  19. Zaidi, S. H., Kaw, J. L.: Effect of general dietary and protein malnutrition on the fibrogenesis caused by silica dust in rats. Brit. J. industr. Med. 27, 250–259 (1970).Google Scholar
  20. Zlatkis, A., Zak, B., Boyle, A. J.: A new method for the direct determination of serum cholesterol. J. Lab. clin. Med. 41, 486–492 (1953).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • G. S. D. Gupta
    • 1
  • J. L. Kaw
    • 1
  • S. H. Zaidi
    • 1
  1. 1.Industrial Toxicology Research CentreLucknowIndia

Personalised recommendations