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An attempt of immunodiagnosis by means of mixed passive hemagglutination (MPHA) test for human pulmonary dirofilariasis

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Abstract

The mixed passive hemagglutination (MPHA) test was used for the first time in an attempt to diagnose human pulmonary dirofilariasis in a comparison with the results of ELISA. Eight of nine patients tested with the MPHA test were positive and one was false-positive; these results coincided exactly with those of ELISA. Qualitative agreement between the MPHA test and ELISA was 90.4% of 374 human sera. The present study indicates that the MPHA test seems to be a promising diagnostic tool for human pulmonary dirofilariasis.

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References

  • Ciferri F (1982) Human pulmonary dirofilariasis in the United States: A critical review. Am J Trop Med Hyg 31:302–308

    Google Scholar 

  • Glickman LT, Grieve RB, Schantz PM (1986) Serologic diagnosis of zoonotic pulmonary dirofilariasis. Am J Med 80:161–164

    Google Scholar 

  • Juji T, Kano K, Milgran F (1972) Mixed agglutination with platelets. Int Arch Allergy Appl Immunol 42:474–484

    Google Scholar 

  • Matsuda H, Tanaka H, Blas BL, Noseñas JS, Tokawa T, Ohsawa S (1984) Evaluation of ELISA with ABTS, 2- 2′-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med 54:131–138

    Google Scholar 

  • Namikawa S, Takeuchi Y, Kimura M, Kasai K, Kashiwagi H, Yoshimura H, Kusagawa M (1986) A resected case of pulmonary dirofilariasis detected on X-ray as a coin lesion. Jpn J Thoracic Dis 24:914–917 (in Japanese with English abstract)

    Google Scholar 

  • Sato M, Koyama A, Iwai K, Kawabata Y, Kojima S (1985) Human pulmonary dirofilariasis with special reference to the ELISA for the diagnosis and follow-up study. Z Parasitenkd 71:561–563

    Google Scholar 

  • Shibata Y, Juji T, Nishizawa Y, Sakamoto H, Ozawa N (1981) Detection of platelet antibodies by a newly developed mixed agglutination with platelets. Vox Sang 41:25–31

    Google Scholar 

  • Voller A, Bidwell DE, Bartlett A (1979) The enzyme linked immunosorbent assay (ELISA). A guide with abstracts of microplate applications. Dynatech Lab Inc, Alexandria Virginia, p 1–125

    Google Scholar 

  • Yoshimura H (1983) Human dirofilariasis in zoonotic parasitoses. In: Zoonotic parasitoses in Japan. Hayashi J, Ishii T, Ohshio Y, Koyama T, Kond S (eds) Bun-eido, Tokyo, pp 284–296 (in Japanese)

    Google Scholar 

  • Yoshimura H, Akao N (1985) Current status of human dirofilariasis in Japan. Int J Zoonoses 12:53–60

    Google Scholar 

  • Yoshimura H, Akao N, Kondo K, Ohnishi Y, Kitagawa M, Kamimura K (1980) Human dirofilariasis in Japan. Case report and review of literature. Int J Zoonoses 7:107–114

    Google Scholar 

  • Yoshimura H, Akao N, Kamimura K (1981) Case reports of infections with Dirofilaria immitis in lung and Wucheria bancrofti in epididymis, diagnosed by cross section morphology of worms in pathological specimens. Jpn J Parasitol 30:381–386

    Google Scholar 

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Ohnishi, Y., Yoshimura, H., Ohyama, T. et al. An attempt of immunodiagnosis by means of mixed passive hemagglutination (MPHA) test for human pulmonary dirofilariasis. Parasitol Res 74, 277–280 (1988). https://doi.org/10.1007/BF00539578

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  • DOI: https://doi.org/10.1007/BF00539578

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