Abstract
Discoid granules were isolated from the tegument of adult Schistosoma mansoni by differential centrifugation of freeze-thaw extracts. Comparison of proteins and glycoproteins from discoid granules, surface membranes and the soluble fraction implied that discoid granules and the surface membrane were functionally linked. The results are discussed in terms of discoid granule function and surface membrane organisation.
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References
Beeley JG (1985) Glycoprotein and proteoglycan techniques. Elsevier, Amsterdam
Carlisle S, Weisberg LS (1978) Schistosoma mansoni: tracer studies in mice. Exp Parasitol 44:124–135
Chappell LH (1974) Methionine uptake by larval and adult Schistosoma mansoni. Int J Parasitol 4:361–369
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Biochem 28:350–356
Ernst SC (1976) Biochemical and cytochemical studies of alkaline phosphatase activity in Schistosoma mansoni. Rice Univ Stud 62:81–95
Hanna REB (1980) Fasciola hepatica: autoradiography of protein synthesis, transport and secretion by the tegument. Exp Parasitol 50:297–304
Hockley DJ, McLaren DJ (1973) Schistosoma mansoni: Changes in the outer membrane of the tegument during development from cercaria to adult worm. Int J Parasitol 3:13–25
Hockley DJ, McLaren DJ, Ward BJ, Nermut MV (1975) A freeze-fracture study of the tegumental membrane of Schistosoma mansoni. Tissue Cell 7:485–496
Kusel JR (1972) Protein composition and protein synthesis in the surface membranes of Schistosoma mansoni. Parasitology 65:55–69
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:680–685
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
MacGregor AN, Stott DI, Kusel JR (1985) Lectin binding to glycoproteins in the surface membrane of Schistosoma mansoni. Mol Biochem Parasitol 16:163–172
McDiarmid SS, Podesta RB, Rahman SM (1982) Preparation and partial characterization of a multilamellar body fraction from Schistosoma mansoni. Mol Biochem Parasitol 5:93–105
McDiarmid SS, Dean LL, Podesta RB (1983) Sequential removal of outer bilayer and apical plasma membrane from the surface epithelial syncytium of Schistosoma mansoni. Mol Biochem Parasitol 7:141–157
McLaren DJ (1980) Schistosoma mansoni: the parasite surface in relation to host immunity. John Wiley, New York
Meryman HT (1974) Freezing injury and its prevention in living cells. Ann Rev Biophys Bioeng 3:341–363
Oaks JA, Cain GC, Mower DA, Raj RK (1983) Comparison of calcium, freeze-thaw and Triton X-100 tegumental disruption/recovery techniques applied to Schistosoma mansoni. J Parasitol 69:519–533
Roberts SM, MacGregor AN, Vojvodic M, Wells E, Crabtree JE, Wilson RA (1983) Tegument surface membranes of adult Schistosoma mansoni: development of a method for their isolation. Mol Biochem Parasitol 9:105–127
Rogers SH, Bueding E (1975) Anatomical localization of glucose uptake by Schistosoma mansoni adults. Int J Parasitol 5:369–371
Senft AW (1968) Studies in proline metabolism by Schistosoma mansoni. Comp Biochem Physiol 27:251–261
Simpson AJG, Schryer MD, Cesari IM, Evans WH, Smithers SR (1981) Isolation and partial characterization of the tegumental outer membrane of adult Schistosoma mansoni. Parasitology 83:163–177
Smith JH, Reynolds ES, von Lichtenberg F (1969) The integument of Schistosoma mansoni. Am J Trop Med Hyg 18:28–49
Wilson RA, Barnes PE (1974) The tegument of Schistosoma mansoni: observations on the formation, structure and composition of cytoplasmic inclusions in relation to tegument function. Parasitology 68:239–258
Wilson RA, Barnes PE (1977) The formation and turnover of the membranocalyx on the tegument of Schistosoma mansoni. Parasitology 74:61–71
Wilson RA, Barnes PE (1979) Synthesis of macromolecules by the epithelial surfaces of Schistosoma mansoni: an autoradiographic study. Parasitology 78:295–310
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MacGregor, A.N., Kusel, J.R. & Wilson, R.A. Isolation and characterisation of discoid granules from the tegument of adult Schistosoma mansoni . Parasitol Res 74, 250–254 (1988). https://doi.org/10.1007/BF00539573
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DOI: https://doi.org/10.1007/BF00539573