Abstract
125I-radiolabelled surface proteins of various developmental stages of Obeliscoides cuniculi were used to analyze serum and gastrointestinal immunoglobulin responses of the host to primary infection with 10000 larvae of the nematode, using a radioimmunoprecipitation method. Serum IgM, IgA, and IgG antibodies reacted mostly with infective larval and adult nematodal surface proteins. The highest binding activity was shown by IgG against the surface of infective larvae on days 56 and 86 after infection. Local (gastric mucosal) antibodies were also predominantly directed against surface antigens of infective larvae and adults, although considerable binding of the fourth-stage larval surface proteins by gastric mucosal IgG occurred 35 days after infection. Physicochemical analysis of the radiolabelled surface antigens revealed that the strongest antigenicity was found in proteins with molecular weights of 40000 and 145000. Nonspecific binding demonstrated in our tests by infective larval surface proteins was associated with particles with a molecular weight of 550000.
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References
Andrews P (1965) The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem J 96:595–606
Bohn A, König W (1985) Characterization of antigens of the nematode Nippostrongylus brasiliensis by monoclonal antibodies. Z Parasitenkd 71:663–672
Dahlgren UIH, Ahlstedt S, Hanson LA (1987) The localization of the antibody response in milk or bile depends on the nature of the antigen. J Immunol 138:1397–1402
Duncan JL, Smith WD, Dargie JD (1978) Possible relationship of levels of mucosal IgA and serum IgG to immune unresponsiveness of lambs to Haemonchus contortus. Vet Parasitol 4:21–27
Helal IB, Wedrychowicz H, Sinski E, Bezubik B (1987) Inhibition phenomena mechanisms in experimental obeliscoidosis in rabbits. II. Local and systemic antibody responses. J Helminthol 61:115–123
Klipstein FA, Engert RF, Clementy JD (1982) Arousal of mucosal secretory immunoglobulin A antitoxin in rats immunised with Escherichia coli heat-labile enteroroxin. Infect Immun 37:1086–1092
Maizels RM, Philipp M, Ogilvie BM (1982) Molecules on the surface of parasitic nematodes as probes of the immune response in infection. Immunol Rev 61:109–136
Maizels RM, Meghiji M, Ogilvie BM (1983) Restricted sets of parasite antigens from the surface of different stages and sexes of the nematode parasite Nippostrongylus brasiliensis. Immunology 48:107–121
Maizels RM, de Savigny D, Ogilvie BM (1984) Characterisation of surface and excretory-secretory antigens of Toxocara canis infective larvae. Parasite Immunol 6:23–37
Maizels RM, Burke J, Sutanto J, Partono F (1986) Secreted and surface antigens from larval stages of Wuchereria bancrofti, the major human lymphatic filarial parasite. Mol Biochem Parasitol 19:27–34
Ogilvie BM, Lee CB, Philipp M, Parkhouse RME (1980) The antigenicity of the nematode surface. In: Israel L, Lagrange P, Salomon JC (eds) Cancer immunology and parasite immunology. Colloq INSERM 97:443–448
Parkhouse RME, Philipp M, Ogilvie BM (1981) Characterisation of surface antigens of Trichinella spiralis infective larvae. Parasite Immunol 3:339–352
Philipp M, Rumjaneck FD (1984) Antigenic and dynamic properties of helminth surface structures. Mol Biochem Parasitol 10:245–268
Philipp M, Worms MJ, McLaren DJ, Ogilvie BM, Parkhouse RM, Taylor PM (1984) Surface protein of a filarial nematode: a major soluble antigen and a host component on the cuticle of Litomosoides carinii. Parasite Immunol 6:63–82
Pierce NF (1978) The role of antigen form and function in the primary and secondary intestinal immune responses to cholera toxin and toxoid in rats. J Exp Med 148:195–206
Pritchard DI, Maizels RM, Behnke JM, Appleby P (1984) Stage specific antigens of Nematospiroides dubius. Immunology 53:325–335
Russell SW, Baker NF, Razes GS (1966) Experimental Obeliscoides cuniculi infections in rabbits: comparison with Trichostrongylus and Ostertagia infections in cattle and sheep. Exp Parasitol 19:163–173
Smith WD, Angus KW (1980) Haemonchus contortus: attempts to immunise lambs with irradiated larvae. Res Vet Sci 29:45–50
Smith WD, Christie MG (1978) Haemonchus contortus: local and serum antibodies in sheep immunised with irradiated larvae. Int J Parasitol 8:219–223
Smith WD, Jackson F, Jackson E, Williams J (1983) Studies on the local response of the lactating ewe infected with Ostertagia circumcincta. J Comp Pathol 93:295–305
Sollod AE, Allen JR (1971) Immunity to Obeliscoides cuniculi: chemical suppression and passive transfer. J Parasitol 57:339–342
Turner BM (1983) The use of alkaline-phosphatase-conjugated second antibody for the visualization of electrophoretically separated proteins recognized by monoclonal antibodies. J Immunol Methods 63:1–6
Wedrychowicz H (1986) Studies on the antigens of Ostertagia circumcincta: III. Somatic and metabolic antigens of adults. Acta Parasitol Pol 30:317–326
Wedrychowicz H, Maclean JM, Holmes PH (1984a) Secretory IgA responses in rats to antigens of various developmental stages of Nippostrongylus brasiliensis. Parasitology 89:145–157
Wedrychowicz H, Abbott EM, Holmes PH (1984b) Use of coproantibody measurement to assess the influence of diet on local immune responses of sheep vaccinated against Haemonchus contortus. Res Vet Sci 36:240–246
Wedrychowicz H, Maclean JM, Holmes PH (1985) Some observations on a possible role of lung and faecal IgA antibodies in immunity of rats to Nippostrongylus brasiliensis. J Parasitol 71:62–69
Worley DE (1963) Experimental studies on Obeliscoides cuniculi, a trichostrongylid stomach worm of rabbits. I. Hostparasite relationship and maintenance in laboratory rabbits. J Parasitol 49:46–50
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Wedrychowicz, H., Sinski, E. & Bezubik, B. Surface antigens of Obeliscoides cuniculi inducing local and systemic humoral responses in infected rabbits. Parasitol Res 74, 386–392 (1988). https://doi.org/10.1007/BF00539463
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DOI: https://doi.org/10.1007/BF00539463