Abstract
The effects of colchicine, taxol, dinitrophenol, sodium azide, TLA-144, and obioactin on Theileria annulata schizonts were tested in vitro and studied by means of light and electron microscopy. Colchicine (0.001–5 ΜM) and taxol (1 ΜM) completely inhibited the mitosis of the host cell. This resulted in higher numbers of schizont nuclei after 96 h, since the division of the parasites was apparently not affected. Increasing numbers of schizont nuclei were also obtained after incubation of parasitized lymphocytes for 7 day at 41‡ C with a daily medium exchange.
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References
BØyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. J Clin Invest 21 [Suppl 97]:77–89
Brown CGD, Stagg DA, Purnell RE, Kanhai GK, Payne RC (1973) Infections and transformation of bovine lymphoid cells in vitro by infective particles of Theileria parva. Nature 245:101–103
Danskin D, Wilde JKH (1976) Simulation in vitro of bovine host cycle of Theileria parva. Nature 261:311–312
Dolan TT (1987) Immunization to control East Coast fever. Parasitol Today 3:4–6
Hulliger L (1965) Cultivation of three species of Theileria in lymphoid cells in vitro. J Protozool 12:649–655
Hulliger L, Brown CGD, Wilde JKH (1966) Transition of developmental stages of Theileria parva in vitro at high temperatures. Nature 211:328–329
Irvin AD (1985) Immunity in theileriosis. Parasitol Today 1:124–128
Malmquist WA, Nyindo MBA, Brown CGD (1970) East Coast fever: cultivation in vitro of bovine spleen cell lines infected and transformed by Theileria parva. Trop Anim Health Prod 2:139–145
Mehlhorn H, Schein E (1984) The piroplasms: life cycle and sexual stages. Adv Parasitol 23:37–103
Morrison WI, Lalor PA, Goddeeris BM, Teale AJ (1986) Theileriosis: antigens and host-parasite interactions. In: Pearson TW (ed) Parasite antigens. New York, pp 337–348
Schein E, Mehlhorn H, Warnecke M (1978) Electron microscopic studies on the schizogony of four Theileria species of cattle (T. parva, T. lawrencei, T. annulata, and T. mutans). Protistologica XIV:337–348
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Supported by DFG
Was held at the 6th Japanese-German Cooperative Symposium on Protozoan Diseases, September 21–25, 1987 Martinsried/München
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Fritsch, F.M., Mehlhorn, H., Schein, E. et al. The effects of drugs on the formation of Theileria annulata merozoites in vitro. Parasitol Res 74, 340–343 (1988). https://doi.org/10.1007/BF00539455
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DOI: https://doi.org/10.1007/BF00539455