Abstract
A 46000 dalton glycoprotein was isolated by extraction of freshly harvested P. falciparum merozoites (FCB1 strain), followed by gel electrophoresis of the extract and electroelution. The antigen is present in the late ring, trophozoite, schizont, and segmenter stages and is localized on the merozoite surface at the end of schizogony. It is not related to the 185000–195000 dalton schizont antigen. An antiserum against the 46000 dalton antigen inhibits invasion of erythrocytes by merozoites. The isolated antigen is identical to the antigen against which monoclonal antibody (mcab) 13.4 is directed.
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Anderson DJ, Adams PH, Hamilton MS, Alexander NJ (1983) Antisperm antibodies in mouse vasectomy sera react with embryonal teratocarcinoma. J Immunol 131:2908–2912
Freeman RR, Holder AA (1983) Surface antigens of malaria merozoites. A high molecular weight precursor is processed to an 83000 mol. wt. form expressed on the surface of Plasmodium falciparum merozoites. J Exp Med 158:1647–1653
Heidrich H-G, Strych W, Mrema JEK (1983) Identification of surface and internal antigens from spontaneously released Plasmodium falciparum merozoites by radioiodination and metabolic labelling. Z Parasitenkd 69:715–725
Heidrich H-G, Strych W, Prehm P (1984) Spontaneously released Plasmodium falciparum merozoites from culture possess glycoproteins. Z Parasitenkd 70:747–751
Holder AA, Freeman RR (1984) The three major antigens on the surface of Plasmodium falciparum merozoites are derived from a single high molecular weight precursor. J Exp Med 160:624–629
Holder AA, Sandhu JS, Hillman I, Davey LS, Nicholls SC, Copper H, Lockyer MJ (1987) Processing of the precursor to the major merozoite surface antigens of Plasmodium falciparum. Parasitology 94:199–208
Howard RF, Stanley HA, Campbell GH, Langreth SG, Reese RT (1985) Two Plasmodium falciparum merozoite surface polypeptides share epitopes with a single Mr 185000 parasite glycoprotein. Mol Biochem Parasitol 17:61–77
Hunkapiller MW, Lujan E, Ostrander F, Hood LE (1983) Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Methods Enzymol 91:227–236
Lyon JA, Geller RH, Haynes JD, Chulay JD, Weber JL (1986) Epitope map and processing scheme for the 195 000 dalton surface glycoprotein of Plasmodium falciparum merozoites deduced from cloned overlapping segments of the gene. Proc Natl Acad Sci USA 83:2989–2993
Lyon JA, Haynes JD, Diggs CL, Chulay JD, Haidaris CG, Pratt-Rossiter J (1987) Monoclonal antibody characterization of the 195 kilodalton major surface glycoprotein of Plasmodium falciparum malaria schizonts and merozoites: Identification of additional processed products and a serotype-restricted repetitive epitope. J Immunol 138:895–901
McBride JS, Heidrich H-G (1987) Fragments of the polymorphic Mr 185000 glycoprotein form the surface of isolated Plasmodium falciparum merozoites from an antigenic complex. Mol Biochem Parasitol 23:71–84
McBride JS, Newbold CI, Anand R (1985) Polymorphism of a high molecular weight schizont antigen of the human malaria parasite Plasmodium falciparum. J Exp Med 161:160–180
Mrema JEK, Langerth SG, Jost RC, Rieckmann KH, Heidrich H-G (1982) Plasmodium falciparum. Isolation and purification of spontaneously released merozoites by nylon membrane sieves. Exp Parasitol 54:285–295
Pasvol G, Wilson RJ, Smalley ME, Brown J (1978) Separation of viable schizont-infected red blood cells of Plasmodium falciparum from human blood. Ann Trop Med Parasitol 72:87–88
Perkins ME (1984) Surface proteins of Plasmodium falciparum merozoites binding to the erythrocyte receptor, glycophorin. J Exp Med 160:788–798
Perrin LH, Merkli B, Gabra MS, Stocker JW, Chizzolini C, Richle R (1985) Immunization with a Plasmodium falciparum merozoite surface antigen induces a partial immunity in monkeys. J Clin Invest 75:1718–1721
Reese RT, Langreth SG, Trager W (1979) Isolation of stages of the human parasite Plasmodium falciparum from culture and from animal blood. Bull WHO 57:53–61
Saul A (1987) Kinetic constraints on the development of a malaria vaccine. Parasite Immunol 9:1–9
Stanley HA, Howard RF, Reese RT (1985) Recognition of a Mr 56 K glycoprotein on the surface of Plasmodium falciparum merozoites by mouse monoclonal antibodies. J Immunol 134:3439–3444
Strych W, Miettinen-Baumann A, Lottspeich F, Heidrich H-G (1987) Isolation and characterization of the 80 000 dalton Plasmodium falciparum merozoite surface antigen. Parasitol Res 73:435–441
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Vernot-Hernandez JP, Heidrich H-G (1985) The relationship to knobs of the 92000 D protein specific for knobby strains of Plasmodium falciparum. Z Parasitenkd 71:41–51
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Miettinen-Baumann, A., Strych, W., McBride, J. et al. A 46000 dalton Plasmodium falciparum merozoite surface glycoprotein not related to the 185000–195000 dalton schizont precursor molecule: isolation and characterization. Parasitol Res 74, 317–323 (1988). https://doi.org/10.1007/BF00539452
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DOI: https://doi.org/10.1007/BF00539452