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The ultrastructure of the cysts of Blastocrithidia triatomae Cerisola et al. 1971 (Trypanosomatidae): A freeze-fracture study

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Abstract

Cysts of Blastocrithidia triatomae from hosts and from in vitro cultures were investigated by freeze-fracture and freeze-etch electron microscopy. No differences in structure were observed between cysts frozen immediately and those fixed and cryoprotected with glycerol. The ultrastructure of all cysts differed considerably from that known for mastigote stages of trypanosomatids: the nuclear pores were surrounded by circular fracture-plane transitions between the inner and outer nuclear membranes; the matrix of the kinetoplast appeared banded; the mitochondrion and the endomembrane system were poorly developed; no flagellar apparatus or basal body could be found; and the subpellicular microtubules characteristic of trypanosomatids were absent. Beneath the surface membrane, a special region, 80–90 nm thick and consisting of several rows of closely packed particles was observed. Intramembrane particle (IMP) distribution on the fracture faces of the surface membrane was highly asymmetric: the ectoplasmic face had very few IMPs, whereas the protoplasmic face contained numerous, closely packed IMPs. No external cyst wall was present.

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References

  • Brooker BE, Ogden CG (1972) Encystment in Bodo caudatus. Protoplasma 74:397–409

    Google Scholar 

  • Cerisola JA, Del Prado CE, Rohwedder R, Bozzini JP (1971) Blastocrithidia triatomae n.sp. found in Triatoma infestans from Argentina. J Protozool 18:503–506

    Google Scholar 

  • Golecki JR, Oelze J (1980) Differences in the architecture of cytoplasmic and intracytoplasmic membranes of three chemotrophically and phytotrophically grown species of the Rhodospirillaceae. J Bacteriol 144:781–788

    Google Scholar 

  • Mehlhorn H, Schaub GA, Peters W, Haberkorn A (1979) Electron microscopic studies on Blastocrithidia triatomae Cerisola et al., 1971 (Trypanosomatidae). Tropenmed Parasitol 30:289–300

    Google Scholar 

  • Peng PL-M, Wallace FG (1982) The cysts of Blastocrithidia triatomae Cerisola et al. 1971. J Protozool 29:464–467

    Google Scholar 

  • Reduth D (1986) In vitro Kultivierung, Entwicklungszyklus und Ultrastruktur von Blastocrithidia triatomae Cerisola et al., 1971 (Trypanosomatidae, Kinetoplastida, Protozoa). Dissertation, University of Freiburg

  • Reduth D, Schaub GA, Pudney M (1984) The cultivation of Blastocrithidia triatomae (Trypanosomatidae) in a cell line of its host Triatoma infestans (Reduviidae). Zentralbl Bakteriol Mikrobiol Hyg [A] 258:383

    Google Scholar 

  • Schaub GA (1988) Developmental time and mortality in larvae of the reduviid bugs Triatoma infestans and Rhodnius prolixus after coprophagic infection with Blastocrithidia triatomae (Trypanosomatidae). J Invertebr Pathol (in press)

  • Schaub GA, Jensen C (1985) Zur biologischen BekÄmpfung einer der gro\en Tropenparasitosen, der Chagas-Krankheit, mit dem Flagellaten Blastocrithidia triatomae. Verh Dtsch Zool Ges 78:190

    Google Scholar 

  • Schaub GA, Pretsch M (1981) Ultrastructural studies on the excystment of Blastocrithidia triatomae (Trypanosomatidae). Trans R Soc Trop Med Hyg 75:168–171

    Google Scholar 

  • Schaub GA, Schurr EA, Reduth D, Meiser A (1984) Isolation of cysts of Blastocrithidia triatomae (Trypanosomatidae) and the infection of the host Triatoma infestans (Reduviidae). J Protozool 31:58A-59A

    Google Scholar 

  • Souto-Padrón T, Goncalves de Lima VMQ, Roitman I, De Souza W (1980) Fine structure study of Leptomonas samueli by the freeze-fracture technique. Z Parasitenkd 62:145–157

    Google Scholar 

  • Tieszen K, Molyneux DH, Abdel-Hafez SK (1985) Ultrastructure of cyst formation in Blastocrithidia familiaris in Lygaeus pandurus (Hemiptera: Lygaeidae). Z Parasitenkd 71:179–188

    Google Scholar 

  • Vickerman K, Preston TM (1976) Comparative cell biology of the kinetoplastid flagellates. In: Lumsden WHR, Evans DH (eds) Biology of the Kinetoplastida. Academic Press, London, vol 1, pp 35–130

    Google Scholar 

  • Vickerman K, Tetley L (1977) Recent ultrastructural studies on trypanosomes. Ann Soc Belg Med Trop 57:441–455

    Google Scholar 

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  1. Institut für Biologie I (Zoologie) der Albert-Ludwigs-Universität, Albertstrasse 21a, D-7800, Freiburg, Federal Republic of Germany

    Dagmar Reduth & G. A. Schaub

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  1. Dagmar Reduth
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  2. G. A. Schaub
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Reduth, D., Schaub, G.A. The ultrastructure of the cysts of Blastocrithidia triatomae Cerisola et al. 1971 (Trypanosomatidae): A freeze-fracture study. Parasitol Res 74, 301–306 (1988). https://doi.org/10.1007/BF00539449

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