Marine Biology

, Volume 87, Issue 2, pp 199–202 | Cite as

External secretion of tetrodotoxin from puffer fishes stimulated by electric shock

  • M. Kodama
  • T. Ogata
  • S. Sato


Four toxic species of puffers (Takifugu pardalis, T. poecilonotus, T. vermicularis and T. niphobles) secreted large amounts of tetrodotoxin into the surrounding water immediately after being stimulated by electric shock. The total amount of toxin secreted ranged from 2 000 to 50 000 MU depending upon the specimen. The amount of toxin secreted seemed to be related to the toxicity of individuals. Non-toxic specimens of T. rubripes did not secrete any toxin. The stimulation always caused inflation of puffers, and this is generally accepted as a repelling behavior of these species. The secreted tetrodotoxin may act as a repellent to predators.


Toxicity Electric Shock Tetrodotoxin Surrounding Water Toxic Species 


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Literature cited

  1. Buchwald, H. D., L. Durham, H. G. Fischer, R. Harada, H. S. Mosher, C. Y. Kao and F. A. Fuhrman: Identity of tarichatoxin and tetrodotoxin. Science, N.Y. 143, 474–475 (1964)Google Scholar
  2. Fallon, W. E. and Y. Shimizu: Electrophoretic analysis of paralytic shellfish toxins. J. environ. Sci. Health. A12, 455–464 (1977)Google Scholar
  3. Halstead, B. W. (Ed.): Poisonous and venomous marine animals of the world. Vol. 3, 1006 pp. Washington: US Government Printing Office (1970)Google Scholar
  4. Hashimoto, Y. (ed.): Marine toxins and other bioactive marine metabolites, 369 pp. Tokyo: Japan Scientific Societies Press 1979Google Scholar
  5. Kawabata, T.: Puffer toxin In: Shokuhin Eisei Kensa Shishin (Manual for the methods of food sanitation testing), Vol. 2, pp 232–240. Ed. by Japan Hygienic Association (in Japanese). Tokyo 1978Google Scholar
  6. Kim, Y. H., G. B. Brown, H. S. Mosher and F. A. Fuhrman: Tetrodotoxin: occurrence in atelopid frogs of Costa Rica. Science, N.Y. 189, 151–152 (1975)Google Scholar
  7. Kodama, M., T. Noguchi, J. Maruyama, T. Ogata and K. Hashimoto: Release of tetrodotoxin and paralytic shellfish poison from puffer liver by RNase. J. Biochem. 93, 243–247 (1983a)Google Scholar
  8. Kodama, M., T. Ogata, T. Noguchi, J. Maruyama and K. Hashimoto: Occurrence of saxitoxin and other toxins in the liver of the purffer fish Takufugu pardalis. Toxicon 21, 897–900 (1983b)Google Scholar
  9. Kodama, M. and T. Ogata: Toxicity of fresh water puffer, Tetrodon leiurus. Bull. Japan. Soc. scient. Fish 50, 1949–1951 (1984)Google Scholar
  10. Matsui, T., H. Sato, S. Hamada and C. Shimizu: Comparison of toxicity of the cultured and wild puffer fish Fugu niphobles. Bull. Japan. Soc. scient. Fish. 48, 253 (1982)Google Scholar
  11. Narita, H., T. Noguchi, J. Maruyama, Y. Ueda, K. Hashimoto, Y. Watanabe and K. Hida: Occurrence of tetrodotoxin in a trumpet shell, “Boshubora” Charonia sauliae. Bull. Japan. Soc. scient. Fish. 47, 935–941 (1981)Google Scholar
  12. Narita, H., T. Noguchi, J. Maruyama, M. Nara and K. Hashimoto: Occurrence of a tetrodotoxin-associated substance in a Gastropod, “Hanamushirogai” Zeuxis squijorensis. Bull. Japan. Soc. scient. Fish. 50, 85–88 (1984)Google Scholar
  13. Noguchi, T. and Y. Hashimoto: Isolation of tetrodotoxin from a goby Gobius criniger. Toxicon 11, 305–307 (1973)Google Scholar
  14. Noguchi, T., J. Maruyama, Y. Ueda, K. Hashimoto and T. Harada: Occurrence of tetrodotoxin in the Japanese ivory shell Babylonia japonica. Bull. Japan. Soc. scient. Fish. 47, 909–913 (1981)Google Scholar
  15. Noguchi, T., H. Narita, J. Maruyama and K. Hashimoto: Tetrodotoxin in the starfish Astropecten polyacanthus, in association with toxification of a trumpet shell, “Boshubora” Charonia sauliae. Bull. Japan. Soc. scient. Fish. 48, 1173–1177 (1982)Google Scholar
  16. Noguchi, T., J. Maruyama, H. Narita and K. Hashimoto: Occurrence of tetrodotoxin in the gastropod mollusk Tutufa lissostoma (frog shell). Toxicon 22, 219–226 (1984)Google Scholar
  17. Scheumack, D. D., M. E. H. Howden, I. Spence and R. J. Quinn: Maculotoxin: a neurotoxin from the venom glands of the octopus Hapalochlaena maculosa. Science, N.Y. 199, 188–189 (1978)Google Scholar
  18. Shimizu, Y. and M. Kobayashi: Apparent lack of tetrodotoxin biosynthesis in captured Taricha torosa and Taricha granulosa. Chem. Pharm. Bull. 31, 3625–3631 (1983)Google Scholar
  19. Tani, I. (Ed.): Nihonsan Fugu no Chudokugakuteki Kenkyu (Toxicological Studies on Japanese Puffers), 103 pp. Tokyo: Teikoku Tosho (in Japanese) 1945Google Scholar
  20. Tsuda, K. and M. Kawamura: The constituents of the ovaries of globefish. VII. Purification of tetrodotoxin by chromatography. (2). J. pharm. Soc. Japan 72, 771–773 (1952)Google Scholar
  21. Woodward, R. B.: The structure of tetrodotoxin. Pure appl. Chem. 9, 49–74 (1964)Google Scholar
  22. Yasumoto, T., Y. Oshima, M. Hosaka and S. Miyakoshi: Occurrence of tetrodotoxin in the ivory shell Babylonia japonica from Wakasa Bay. Bull. Japan. Soc. scient. Fish. 47, 929–934 (1981)Google Scholar
  23. Yasumoto, T., M. Nakamura, Y. Oshima and J. Takahata: Construction of a continuous tetrodotoxin analyzer. Bull. Japan. Soc. scient. Fish. 48, 1481–1483 (1982)Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • M. Kodama
    • 1
  • T. Ogata
    • 1
  • S. Sato
    • 1
  1. 1.Laboratory of Marine Biological Chemistry, School of Fisheries SciencesKitasato UniversityIwateJapan

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