Alveolar macrophages in pulmonary lavage harvested with and without previous lung massage
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The morphology, phagocytosis and metabolic activity of alveolar macrophages harvested by two lung-washing techniques, with and without lung massage, were compared. The number of cells in each lung lavage was counted. Lung tissue was examined histologically after repeated lavages and massages.
There was a higher proportion of larger cells among the cells harvested without massage. The number of cells in the first wash after massage was 7.1±2.25 (mean ±standard deviation) compared to 1.9±0.4 without massage. The number of phagocytically active cells was significantly higher in lavages without massage than in lavages after lung massage (P<0.002). However, the number of engulfed bacteria per cell was similar in both groups. Proteosynthesis was very intensive in both cell groups, judging by the high uptake of 14C-labelled l-leucine. There was no significant difference in the uptake of the RNA precursor, 14C uracil. However, the uptake of 14C-uridin was significantly higher in alveolar macrophages harvested after lung massage (P<0.001), as was the activity of glucose-6-phosphate dehydrogenase (P<0.001). Light microscopy revealed no substantial damage of lung tissue after lavage and/or massage.
In view of the data obtained it is suggested that the increased release of alveolar macrophages following massage in an intact organism is due to younger, immature forms, which are not yet phagocytically active. They differ from cells recovered without massage in their very high biosynthetic activity. The findings also suggest that maturation of alveolar macrophages takes place within the lung.
Key wordsLung lavage Lung massage Alveolar macrophages Functional activity Metabolic activity
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- 1.Bhagwat, A. G., Conen, P. E.: Characterization of “free alveolar cells” in experimental adjuvant induced pneumonia. Arch. Path. 88, 21–29 (1969)Google Scholar
- 2.Bowden, D. H., Adamson, I. Y. R., Grantham, J. P., Wyatt, J. P.: Origin of the lung macrophages. Arch. Path. 88, 540–546 (1969)Google Scholar
- 3.Brain, J. D., Frank, N. R.: Recovery of free cells from rat lungs by repeated washings. J. appl. Physiol. 25, 63–69 (1968)Google Scholar
- 4.Collet, A. J.: Experimental stimulation of alveolar macrophage production by Corynebacterium anaerobium and its quantitative evaluation. J. Reticuloendothel Soc. 9, 424–446 (1971)Google Scholar
- 5.Friedmann, M. M., Kahn, B. S., Lapan, B.: Dehydrogenase of regenerating red blood cells in phenylhydrazine anaemia. Clin. Chem. 3, 489–492 (1962)Google Scholar
- 6.Harris, J. O., Swenson, E. W., Johnson, J. E.: Human alveolar macrophages: Comparison of phagocytic ability, glucose utilization and ultrastructure in smokers and nonsmokers. J. clin. Invest. 49, 2086–2096 (1970)Google Scholar
- 7.Kahring, C., Rapoport, S.: Atmung und Glucose-Phosphatdehydrogenase-AktivitÄt roter Blutkörperchen in verschiedenen Reifungs- und Alterungs-stadien. Folia haemat. (Lpz.) 3, 408–415 (1962)Google Scholar
- 8.Kilburn, K. H.: Alveolar clearance of particles. A bulfrog lung model. Arch. environna. Hlth 18, 556–563 (1969)Google Scholar
- 9.Klosterkötter, W., Einbrodt, H. J.: 18 Untersuchungen Über den Einflu\ von Quarz auf die Retention, Penetration und Elimination eines inerten Staubes. Sonderdruck aus „Ergebnisse von Untersuchungen auf dem Gebiet der Staub- und SilikosebekÄmpfung im Steinkohlenbergbau“, Bd. 5, S. 101–104. Detmold: Bösmann 1965Google Scholar
- 10.LaBelle, C. W., Brieger, H.: The fate of inhaled particles in the early post-exposure period. II. The role of pulmonary phagocytes. Arch. environm. Hlth 1, 423–427 (1960)Google Scholar
- 11.Marks, P. A.: A relationship between human erythrocyte aging in vivo and the activities of glucose-6-phosphatdehydrogenase. J. clin. Invest. 6, 913 (1957)Google Scholar
- 12.Massaro, D., Handler, A., Bottons, L.: Alveolar cells: Protein biosynthesis. Amer. Rev. resp. Dis. 96, 957–961 (1967)Google Scholar
- 13.Myrvik, Q. N., Evans, D. G.: Metabolic and immunologic activities of alveolar macrophages. Arch. environm. Hlth 14, 92–96 (1967)Google Scholar
- 14.Myrvik, Q. N., Leake, E. S., Farris, E.: Studies on pulmonary alveolar macrophages from the normal rabbit: A technique to procure them in a high state of purity. J. Immunol. 86, 128–132 (1961)Google Scholar
- 15.Sanders, C. L., Adee, R. R.: Phagocytosis of inhaled plutonium oxide- 239Pu particles by pulmonary macrophages. Science 162, 918–920 (1968)Google Scholar
- 16.Sklenský, B.: The effect of aerosol inhalation on the pulmonary clearance in rats, dusted with the inert and fibroplastic dust (Czech.). Pracov. Lék. 10, 448–452 (1965)Google Scholar
- 17.Vlčková, A.: Some biochemical reactions of alveolar macrophages related to pulmonary clearance (Slovak.). Thesis, Slovak Acad. Sci., Bratislava (1968)Google Scholar
- 18.Vlčková, A., Reichrtová, E., Ferin, J., Urbánková, G.: Changes in the elimination of particles from the lungs in the state of stress (Slovak.). Ann. Rep. Res. Programme, Slovak Academy Sci., Bratislava (1970)Google Scholar