Biophysics of structure and mechanism

, Volume 9, Issue 2, pp 89–93 | Cite as

Intercellular communication, three-dimensional cell contact and radiosensitivity

  • H. Dertinger
  • G. Hinz
  • K. H. Jakobs
Article

Abstract

When electrically coupled mammalian cells are cultured as spherical clones (spheroids) and exposed to ionizing radiation they are less radiosensitive than monolayers of the same cell line. Investigations into the possible role of coupling (gap junctions) and three-dimensional contact in the expression of this phenomenon revealed
  1. 1)

    a correlation between cell coupling and the activity of adenylate cyclase in monolayers,

     
  2. 2)

    a sharp drop of cyclase activity in spheroids of coupled cells compared to monolayers, and

     
  3. 3)

    a decrease of coupling with age (“maturation”) of the spheroids.

     

These results suggest profound physiological alterations in communicating cells induced under conditions of tight three-dimensional contact as a possible cause for the reduced radiosensitivity of spheroids.

Key words

Intercellular communication Spheroids Adenylate cyclase Radiosensitivity 

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References

  1. Dertinger H, Hülser D (1981) Increased radioresistance of cells in cultured multicell spheroids. I. Dependence on cellular interaction. Radiat Environ Biophys 19: 101–107Google Scholar
  2. Durand RE, Sutherland RM (1973) Growth and radiation survival characteristics of V79-171b Chinese hamster cells: A possible influence of intercellular contact. Radiat Res 56: 513–527Google Scholar
  3. Guichard M, Dertinger H, Malaise EP (1982) Radiosensitivity of four human tumor xenografts. Influence of hypoxia and cell-cell contact. Radiat Res, in pressGoogle Scholar
  4. Hinz G (1982) Erhöhte Strahlenresistenz von Sphäroiden aus ionisch gekoppelten Zellen. Zytogenetische und biochemische Untersuchungen. Thesis, University of HeidelbergGoogle Scholar
  5. Hülser DF, Webb DJ (1973) Relation between ionic coupling and morphology of established cells in culture. Exp Cell Res 80: 210–222Google Scholar
  6. Jacobs KH, Saur W, Schultz G (1976) Reduction of adenylate cyclase activity in lysates of human platelets by the α-adrenergic component of epinephrine. J Cyclic Nucleotide Res 2: 381–392Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • H. Dertinger
    • 1
  • G. Hinz
    • 1
  • K. H. Jakobs
    • 2
  1. 1.Kernforschungszentrum KarlsruheInstitut für Genetik und für Toxikologie von SpaltstoffenKarlsruhe 1Federal Republic of Germany
  2. 2.Pharmakologisches Institut der Universität HeidelbergHeidelbergFederal Republic of Germany

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