Distribution of postsynthetic methylation sites in Physarum histone H1

Abstract

Using limited chymotrypsin and trypsin digestion of isolated Physarum histone H1 labeled in vivo in postsynthetically added N^ε-methyl groups of lysine we show that:

• - there is no postsynthetic methylation in the central globular domain of H1,

• - a moderate number of methylated sites occurs in the N-terminal fragment and the part of the C-terminal fragment directly adjacent to the globular domain (the main site of interphase phosphorylation),

• - the most intensively methylated region occurs within the sequence located in an extended part of the C-terminal fragment, distant to the globular domain and the main site of interphase phosphorylation.