Abstract
The osmotic water outflow of large multilamellar liposomes containing α1-acid glycoprotein was measured at a temperature near the lipid's phase transition temperature. The liposomes were formed from a mixture of DPPC, cholesterol and glycoprotein in molar ratios 100:20:1, by continuous sucrose density gradient centrifugation. These liposomes captured 35% of the radiolabeled glycoprotein. The temperature-dependent experiments showed that near phase transition temperature the initial rate of water outflow increased drastically in comparison with glycoprotein free liposomes incubated in buffer containing glycoprotein. We suggested that eventual a channel mechanism may be involved due to spontaneous incorporation of glycoprotein into the bilayer.
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NeitchevVZ & JungWK (1986) Molec. Biol. Rep. 11: 87–92
NeitchevVZ, KostadinovAP & BideaudFA (1986) Int. J. Biochem. 18: 459–464
BlokMC, vanDeenenLLM & DeGierJ (1976) Biochim. Biophys. Acta 433: 1–3
vanDeenenLLM et al. (1975) Ann. N.Y. Acad. Sci. 264: 124–141
BanghamAD, DeGierJ & GrevilleGD (1976) Chem. Phys. Lipids 1: 235–246
KassaiM, KometaniT & FukumotoS (1979) J. Membrane Biol. 51: 311–324
MaceyRJ (1984) Am. J. Physiol. 246: 196–204
MlekodayJH, MooreRL & LevitDG (1983) J. Gen. Physiol. 81: 213–220
RabonE, TakeguchiN & SalomonAK (1981) J. Gen. Physiol. 77: 549–570
TerwilligerTC & SachsG (1980) J. Membrane Biol. 53: 109–117
WormanHJ & FieldM (1985) J. Membrane Biol. 87: 233–239
YeaglePL (1984) J. Membrane Biol. 78: 201–210
KahovcovaJ & OdavicR (1969) J. Chromatography 40: 90–95
GangulyM, CarnighanH & WestpalU (1967) Biochemistry 6: 2803–2814
KochAL (1981) Biochim. Biophys. Acta 51: 429–411
KochAL (1968) J. Theor. Biol. 18: 133–156
NeitchevVZ & KostadinovAP (1987) Molec. Biol. Rep. 12: 253–258
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Neitchev, V.Z., Bideaud, F.A. Temperature-dependent osmotic permeability in glycoproteion containing liposomes. Mol Biol Rep 13, 85–89 (1988). https://doi.org/10.1007/BF00539055
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DOI: https://doi.org/10.1007/BF00539055