Skip to main content
SpringerLink
Log in

‘Conservative’ and ‘variable’ clusters of Alu-family DNA repeats in human chromosomes

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

The distribution of Alu-family DNA repeats (AFRs) in chromosomes of phytohaemagglutinin-stimulated peripheral blood lymphocytes of four normal donors and non-stimulated bone marrow cells of four patients with acute leukemia (ALL and ANLL) was studied by in situ hybridization using DNA of recombinant phage lambda containing multiple inserts of AFR as a probe. Over some chromosome bands (14cen, 16p13, 16cen) from normal donors and from leukemic patients clusters of silver grains were detected. Over other three bands (3q26, 8p11-p12 and 14q24) the clusters were found only in chromosomes from the four acute leukemia patients, and were absent from chromosomes of healthy donors. The results suggest non-random long-range distribution of AFRs in human chromosomes, and somatic variations in the distribution of the repeats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. JelinekWR & SchmidCW (1982) Ann. Rev. Biochem. 51: 813–844

    Google Scholar 

  2. CalabrettaB, RobbersonDL, Barrera-SaldanaHA, LambronT & SaundersGF (1982) Nature 296: 219–225

    Google Scholar 

  3. FilatovLV, MamyevaSE & TomilinNV (1987) Molec. Biol. Rep. 12: 117–121

    Google Scholar 

  4. RubinC, HouckCM, DeiningerPL, FriedmannT & SchmidCW (1980) Nature 284: 372–374

    Google Scholar 

  5. YunisJJ (1981) Human Genet. 56: 293–298

    Google Scholar 

  6. LehrmanMA, GoldsteinJL, RusselDW & BrownMS (1987) Cell 48: 827–835

    Google Scholar 

  7. RowleyJD & PotterD (1976) Blood 47: 705–721

    Google Scholar 

  8. MitelmanF, NilssonPG, BrandtL, AlimenaG, GastaldiR & DallapiccolaB (1981) Cancer Genet. Cytogenet 4: 197–214

    Google Scholar 

  9. YunisJJ, BloomfieldCD & EnsrudK (1981) New Engl. J. Med. 305: 135–139

    Google Scholar 

  10. BeitelLK, ChamberlainJW, BenchimolS, LamT, FriceG & StannersCP (1986) Nucleic Acids Res. 14: 3391–3408

    Google Scholar 

  11. ChamberlainJW, HendersonG, ChangMW-M, LamT, DignardD, LingV, PriceGB & StannersCP (1986) Nucleic Acids Res. 14: 3409–3424

    Google Scholar 

  12. BarkerPE, RabinM, WatsonM, BregWR & RuddleFH (1984) Proc. Natl. Acad. Sci. USA 81: 5826–5830

    Google Scholar 

  13. BernardiG, OlofssonB, FilipskiJ, ZerialM, SalinasJ, CunyG, Meunier-RotivalM & RodierF (1986) Science 228: 953–958

    Google Scholar 

  14. Maniatis T, Fritsch EF & Sambrook J (1982) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor.

Download references

Author information

Authors and Affiliations

  1. Institute of Cytology, The Academy of Sciences of the USSR, 194064, Leningrad, USSR

    L. V. Filatov, S. E. Mamayeva & N. V. Tomilin

Authors
  1. L. V. Filatov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. E. Mamayeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. V. Tomilin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Filatov, L.V., Mamayeva, S.E. & Tomilin, N.V. ‘Conservative’ and ‘variable’ clusters of Alu-family DNA repeats in human chromosomes. Mol Biol Rep 13, 79–84 (1988). https://doi.org/10.1007/BF00539054

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00539054

Key words

Search

Navigation