Molecular Biology Reports

, Volume 13, Issue 2, pp 65–71 | Cite as

Synthesis of functional bovine opsin in insect cells under control of the baculovirus polyhedrin promoter

  • J. J. M. Janssen
  • W. J. M. van de Ven
  • W. A. H. M. van Groningen-Luyben
  • J. Roosien
  • J. M. Vlak
  • W. J. de Grip
Article

Abstract

In vitro expression of cDNA encoding bovine opsin is accomplished using the baculovirus expression vector system. Full-length opsin was synthesized which was recognized by poly- and monoclonal antisera raised against bovine rhodopsin. Upon infection with a recombinant virus, 1×106 insect cells produced up to 3 μg opsin. Incubation of the in vitro synthesized opsin with 11-cis retinal produced a hydroxylamine-stable, photosensitive pigment.

Key words

baculovirus expression system immunocytochemistry in vitro expression opsin regeneration 

Abbreviations

dpi

days post infection

pfu

plaque forming units

AcNPV

Autographa californica nuclear polyhedrosis virus

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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • J. J. M. Janssen
    • 1
  • W. J. M. van de Ven
    • 2
  • W. A. H. M. van Groningen-Luyben
    • 1
  • J. Roosien
    • 3
  • J. M. Vlak
    • 3
  • W. J. de Grip
    • 1
  1. 1.Department of Biochemistry, Center of Eye ResearchUniversity of NijmegenNijmegenThe Netherlands
  2. 2.Dept. of Biochemistry, Molecular Oncology SectionUniversity of NijmegenNijmegenThe Netherlands
  3. 3.Dept. of VirologyAgricultural UniversityWageningenThe Netherlands

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