Mammalian Genome

, Volume 6, Issue 11, pp 802–804 | Cite as

Refined mapping of caltractin in human Xq28 and in the homologous region of the mouse X Chromosome places the gene within the bare patches (Bpa) and striated (Str) critical regions

  • A. Chatterjee
  • T. Tanaka
  • J.E. Parrish
  • G.E. Herman
Original Contributions

Abstract

Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. A human caltractin cDNA (CALT) has recently been mapped by fluorescence in situ hybridization (FISH) to Xq28. We report here refined mapping of the human CALT gene and its murine homolog between the loci DXS1104 (DXHXS1104) and DXS52 (DXHXS52) by PCR and Southern analysis of YACs and somatic cell hybrids from the region in both species. These mapping studies place the gene within the critical region for the murine X-linked dominant, male lethal mutations bare patches and striated.

Keywords

Somatic Cell Structural Component Critical Region Mapping Study Cell Hybrid 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Angel, T., Faust, C., Gonzales, J., Kenwrick, S., Lewis, R.A., Herman, G. (1993). Genetic mapping of the X-linked dominant mutations striated (Str) and bare patches (Bpa) to a 600-kb region of the mouse X chromosome: implications for mapping human disorders in Xq28. Mamm. Genome 4, 171–176.Google Scholar
  2. Chatterjee, A., Faust, C.J., Molinary-Storey, L., Kiochis, P., Poustka, A., Herman, G.E. (1994). A 2.3-Mb yeast artificial chromosome contig spanning from Gabra3 to G6pd on the mouse X chromosome. Genomics 21, 49–57.Google Scholar
  3. de Gouyon, B., Chatterjee, A., Herman, G.E. (1994). Fine genetic and physical mapping of thirteen mouse X chromosome microsatellites. Mouse Genome 92, 516–519.Google Scholar
  4. Faust, C.J., Levinson, B., Gitschier, J., Herman, G.E. (1992). Extension of the physical map in the region of the mouse X chromosome homologous to human Xq28 and identification of an exception to conserved linkage. Genomics 13, 1289–1295.Google Scholar
  5. Freije, D., Schlessinger, D. (1992). A 1.6-Mb contig of yeast artificial chromosomes around the human factor VIII gene reveals three regions homologous to probes for the DXS115 locus and two for the DXYS64 locus. Am. J. Hum. Genet. 51, 66–80.Google Scholar
  6. Herman, G.E., Berry, M., Munro, E., Craig, I.W., Levy, E.R. (1991). The construction of human somatic cell hybrids containing portions of the mouse X chromosome and their use to generate DNA probes via interspersed repetitive sequence polymerase chain reaction. Genomics 10, 961–970.Google Scholar
  7. Lee, V.D., Huang, B. (1993). Molecular cloning and centrosomal localization of human caltractin. Proc. Natl. Acad. Sci. USA 90, 11039–11043.Google Scholar
  8. Nelson, D.L., Ballabio, A., Victoria, M.F., Pieretti, M., Bies, R.D., Gibbs, R.A., Maley, J.A., Chinault, A.C., Webster, T.D., Caskey, C.T. (1991). Alu-primed polymerase chain reaction for regional assignment of 110 yeast artificial chromosome clones from the human X chromosome: identification of clones associated with a disease locus. Proc. Natl. Acad. Sci. USA 88, 6157–6161.Google Scholar
  9. Palmieri, G., Romano, G., Ciccodicola, A., Casamassimi, A., Campanile, C., Esposito, T., Cappa, V., Lania, A., Johnson, S., Reinbold, R., Poustka, A., Schlessinger, D., D'Urso, M. (1994). YAC contig organization and CpG island analysis in Xq28. Genomics 24, 149–158.Google Scholar
  10. Rogner, U.C., Kioschis, P., Wilke, K., Gong, W., Pick, E., Dietrich, A., Zechner, U., Hameister, H., Pragliola, A., Herman, G.E., Yates, J.R.W., Lehrach, H., Poustka, A. (1994). A YAC clone map spanning 7.5 megabases of human chromosome band Xq28. Hum. Mol. Genet. 12, 2137–2146.Google Scholar
  11. Tanaka, T., Okui, K., Nakamura, Y. (1994). Assignment of the human caltractin gene (CALT) to Xq28 by fluorescence in situ hybridization. Genomics 24, 609–610.Google Scholar
  12. Warren, S.T., Knight, S.J.L., Peters, J.F., Stayton, C.L., Consalez, G.G., Zhang, F. (1990). Isolation of the human chromosomal band Xq28 within somatic cell hybrids by fragile X site breakage. Proc. Natl. Acad. Sci. USA 87, 3856–3860.Google Scholar
  13. Willard, H.F., Cremers, F., Mandel, J.-L., Monaco, A.P., Nelson, D.L., Schlessinger, D. (1994). Report of the fifth international workshop on human X chromosome mapping. Cytogenet. Cell Genet. 67, 295–358.Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1995

Authors and Affiliations

  • A. Chatterjee
    • 1
  • T. Tanaka
    • 2
    • 3
  • J.E. Parrish
    • 1
  • G.E. Herman
    • 1
  1. 1.Department of Molecular and Human Genetics, One Baylor Plaza 821TBaylor College of MedicineHoustonUSA
  2. 2.Laboratory of Molecular Medicine, Institute of Medical Science, Faculty of MedicineUniversity of TokyoTokyoJapan
  3. 3.The Third Department of Internal Medicine, Faculty of MedicineUniversity of TokyoTokyoJapan

Personalised recommendations