Summary
The granulosa and the luteal cells have been studied in the ovary of the adult laying hen by histochemistry, autoradiography, polarized light and electron microscopy. It is concluded that the granulosa cells are specialized for the synthesis of proteins, whereas the luteal cells mainly secrete steroids. These conclusions have been tested by examining the cells under three naturally-occurring physiological conditions. (a) In the discharged follicles, the granulosa cells exhibit degenerative changes, and the luteal cells show reduced reactions in the tests for steroids. (b) In the ovaries of old, off-lay hens, the granulosa cells resemble those of the laying hen; the luteal cells however, whilst still showing positive reactions for steroids, have undergone morphological changes, some of which suggest that the cells are atrophic. (c) Luteal are recognisable in embryonic ovaries as early as nine days of incubation. By fifteen days, these cells show strongly positive reactions in tests for steroids.
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References
Aitken, R. N. C.: Post ovulatory development of ovarian follicles in the domestic fowl. Res. Vet. Sci. 7, 138–142 (1965).
Bellairs, R.: Cell death in chick embryos studied by electron microscopy. J. Embryol. exp. Morph. 11, 697–714 (1963).
Bellairs, R.: The relationship between oocyte and follicle in the hen's ovary as shown by electron microscopy. J. Embryol. exp. Morph. 13, 215–233 (1965).
Boucek, R. J., Györi, E., Alvarez, R.: Steroid dehydrogenase reactions in developing chick adrenal and gonadal tissues. A histochemical study. Gen. comp. Endocr. 7, 292–303 (1966).
Boucek, R. J., Savard, K.: Steroid formation by the avian ovary in vitro (Gallus domesticus). Gen comp. Endocr. 15, 6–11 (1970).
Brachet, J.: La détection histochimique et le microdosage des acides pentosenucléiques. Enzymologia 10, 87–96 (1941).
Chieffi, G., Botte, V.: The distribution of some enzymes involved in the steroidogenesis of hen's ovary. Experientia (Basel) 21, 16–17 (1965).
Christensen, A. K., Gillim, S. W.: The correlation of fine structure and function in steroid secreting cells, with emphasis on those of the gonads. In: The gonads (ed. K. W. McKerns), p. 415–488. New York: Appleton-Century Crofts 1969.
Dahl, E.: Studies of the fine structure of ovarian interstitial tissue. 2. The ultrastructure of the thecal gland of the domestic fowl. Z. Zellforsch. 109, 195–211 (1970a).
Dahl, E.: Studies of the fine structure of ovarian interstitial tissue. 3. The innervation of the thecal gland of the domestic fowl. Z. Zellforsch. 109, 212–226 (1970b).
Dahl, E.: Studies of the fine structure of ovarian interstitial tissue. 4. Effects of steroids on the thecal gland of the domestic fowl. Z. Zellforsch. 113, 111–132 (1971a).
Dahl, E.: Studies of the fine structure of ovarian interstitial tissue. 5. Effects of gonadotropins on the thecal gland of the domestic fowl. Z. Zellforsch. 113, 135–156 (1971b).
Davis, D. E.: The regression of the avian post-ovulatory follicle. Anat. Rec. 82, 297–307 (1942).
Dempsey, E. W., Bassett, D. L.: Observations on the fluorescence, birefringence and histochemistry of the rat ovary during the reproductive cycle. Endocrinology 33, 384–401 (1943).
Fell, H. B.: Histological studies on the gonads of the fowl. II. The histogenesis of the so-called “luteal” cells in the ovary. J. Soc. exp. Biol. 1, 293–312 (1924).
Floquet, A., Grignon, G.: Étude histologique du follicle post-ovulataire chez la Poule. C. R. Soc. Biol. (Paris) 158, 132–135 (1964).
Gilbert, A. R.: Formation of the egg in the domestic chicken. Advanc. Reprod. Physiol. 2, 111–180 (1967).
Greenfield, M. L.: The oocyte of the domestic chicken shortly after hatching, studied by electron microscopy. J. Embryol. exp. Morph. 15, 297–316 (1966).
Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27, 137A (1965).
Marshall, A. J., Coombs, C. J. F.: The interaction of environmental, internal and behavioural factors in the rook, Corvus f. frugilegus Linnaeus. Proc. Zool. Soc. (Lond.) 128, 545–589 (1957).
Narbaitz, R., de Robertis, E. M., Jr.: Postnatal evolution of steroidogenic cells in the chick ovary. Histochemie 15, 187–193 (1968).
Narbaitz, R., Kolodny, L.: Δ5-3β-hydroxysteroid dehydrogenase in differentiating chick gonads. Z. Zellforsch. 63, 612–617 (1964).
Narbaitz, R., Sabatini, M. T.: Histochemical demonstration of cholesterol in differentiating chick gonads. Z. Zellforsch. 59, 1–5 (1963).
Palade, G. E.: A study of fixation for electron microscopy. J. exp. Med. 95, 285–298 (1952).
Pearl, E., Boring, A. M.: Corpus luteum of the ovary of the chicken. Amer. J. Anat. 23, 1–35 (1918).
Pearse, A. G. E.: Histochemistry. London: Churchill 1968.
Reynolds, E. S.: The use of lead citrate at high pH as an electron dense stain in electron microscopy. J. Cell Biol. 17, 208 (1963).
Romanoff, A. L.: The avian embryo. New York: Macmillan 1960.
Samuels, L. T.: Metabolism of steroid hormones. In: Metabolic pathways (ed. D. M. Greenberg), vol. I, p. 431–480. New York: Academic Press 1960.
Saunders, J. W., Jr.: Death in embryonic systems. Science N. Y. 154, 604–612 (1966).
Slautterback, J.: Cytoplasmic microtubules. I. Hydra. J. Cell. Biol. 18, 367–388 (1963).
Tienhoven, A. van: Endocrinology and reproduction in birds. In: Sex and internal secretions (ed. W. C. Young), p. 1088–1169. London: Bailliere, Tindall and Cox 1961.
Woods, J. E., Domm, L. V.: A histochemical identification of the androgen-producing cells in the gonads of the domestic fowl and albino rat. Gen. comp. Endocr. 7, 559–570 (1966).
Wyburn, G. M., Baillie, A. H.: Some observations on the fine structure and histochemistry of the ovarian follicle of the fowl. In: Physiology of the domestic fowl (eds. C. Horton-Smith and E. C. Amoroso). Edinburgh-London: Oliver & Boyd 1966.
Wyburn, G. M., Johnston, H. S., Aitken, R. N. C.: Fate of the granulosa cells in the hen's follicle. Z. Zellforsch. 72, 53–65 (1966).
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Peel, E.T., Bellairs, R. Structure and development of the secretory cells of the hen's ovary. Z. Anat. Entwickl. Gesch. 137, 170–187 (1972). https://doi.org/10.1007/BF00538789
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DOI: https://doi.org/10.1007/BF00538789