Molecular and General Genetics MGG

, Volume 234, Issue 3, pp 489–493 | Cite as

Development of a transformation system for the thermophilic fungus Talaromyces sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker

  • Sanjay Jain
  • Henri Durand
  • Gerard Tiraby
Article

Summary

A transformation system for the thermophilic cellulolytic fungus Talaromyces sp. CL240 has been developed, using the phleomycin resistance gene from Streptoalloteichus hindustanus (Sh ble) as a dominant selectable marker. The plasmids (pAN8-1 and pUT720) carrying the Sh ble gene under the control of the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter, allowed selection of phleomycin-resistant transformants. A new promoter sequence cloned from chromosomal DNA of Trichoderma reesei (pUT737) was also able to drive efficient expression of the Sh ble gene in Talaromyces sp. CL240, resulting in the selection of transformants that were highly resistant to phleomycin.

Key words

Recombinant DNA Shotgun cloning Filamentous fungi Promoter sequence 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Sanjay Jain
    • 1
  • Henri Durand
    • 2
  • Gerard Tiraby
    • 1
  1. 1.Laboratoire de Microbiologie et Génétique Appliqués du C.N.R.S., C.R.B.G.C.University Paul SabatierToulouse CédexFrance
  2. 2.Laboratoire CAYLAToulouse CédexFrance

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