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Mutations in the cdc10 start gene of Schizosaccbaromyces pombe implicate the region of homology between cdc10 and SWl6 as important for p85cdc10 function

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Summary

The cdc10 gene of the fission yeast Schizosaccharomyces pombe is required for traverse of start and commitment to the mitotic cell division cycle rather than other fates. The product of the gene, p85cdc10, is a component of a factor that is thought to be involved in regulating the transcription of genes that are required for DNA synthesis. In order to define regions of the p85cdc10 protein that are important for its function a fine structure genetic map of the cdc10 gene was derived and the sequences of 13 cdc10 ts mutants determined. The 13 mutants tested define eight alleles. Eleven of the mutants are located in the region that contains the two copies of the cdc10/SW16 repeat motif, implicating it as important for p85cdc10 function.

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Authors and Affiliations

  1. Unité de Recherche sur le cycle cellulaire, ISREC, Chemin des Boveresses 155, CH-1066, Epalinges, Switzerland

    Alexandre Reymond, Susanne Schmidt & Viesturs Simanis

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  1. Alexandre Reymond
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  2. Susanne Schmidt
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  3. Viesturs Simanis
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Reymond, A., Schmidt, S. & Simanis, V. Mutations in the cdc10 start gene of Schizosaccbaromyces pombe implicate the region of homology between cdc10 and SWl6 as important for p85cdc10 function. Molec. Gen. Genet. 234, 449–456 (1992). https://doi.org/10.1007/BF00538705

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