Advertisement

Bestimmung der Eiweißbindung verschiedener Herzglykoside mittels Sephadex®-Gelfiltration

  • Klaus Kuschinsky
Article

Schlüsselwörter

Herzglykoside Eiweißbindung Sephadex-Gelfiltration 

Protein binding of different cardiac glycosides, determined by means of the sephadex gelfiltration method

Summary

The binding of tritium labelled cardiac glycosides to plasma proteins was investigated by means of the Sephadex gel-filtration method. After pre-saturation with water, Sephadex G 25 was incubated with an excess of guinea pig plasma which contained 3H-labelled heart glycosides. In this procedure, the Sephadex G 25 could not disturb the equilibrium between the free and protein-bound glycoside to a noticeable extent. The uptake of 3H-glycosides by Sephadex from serum was compared with that from a TRIS-buffered 0.9% NaCl-solution containing the drug in the same concentration as the serum or plasma. From the ratio of both values the amount of protein-bound glycoside could be calculated. Approximately 90% of the 3H-digitoxin incubated with guinea pig plasma or with human serum became bound to protein; for 3H-digoxin, this figure was about 55%. No significant protein binding could be established for 3H-ouabain, neither in guinea pig plasma nor in human serum. In contrast to previous investigators, who used toxic concentrations of the glycosides, the present studies have been carried out with glycoside concentrations within the therapeutic range. The results have been compared with those of previous authors.

Key-Words

Cardiac Glycosides Protein Binding Sephadex Gelfiltration 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Doherty, J. E., W. H. Perkins, and M. C. Wilson: Studies with tritiated digoxin in renal failure. Amer. J. Med. 37, 536 (1964).Google Scholar
  2. Haarmann, W., K. Korfmacher u. L. Lendle: Bedingungen der Bindung von Digitalisglykosiden an die Serumeiweißstoffe. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 194, 229 (1940).Google Scholar
  3. Hattingberg, H. M., v., u. W. Klaus: Trennung des proteingebundenen vom frei diffiniblen Calcium im Serum durch Sephadex. Klin. Wschr. 4, 499 (1966).Google Scholar
  4. Kuschinsky, K., H. Lüllmann, and P. A. van Zwieten: A comparison of the accumulation and release of 3H-ouabain and 3H-digitoxin. Brit. J. Pharmacol. (im Druck).Google Scholar
  5. Scholtan, W.: Vergleichende quantitative Bestimmung der Eiweißbindung von Chemotherapeutica mittels Sephadex und Dialyse. Arzneimittel-Forsch. 14, 146 (1964).Google Scholar
  6. K. Schlossmann u. H. Rosenkranz: Bestimmung der Eiweißbindung von Digitalispräparaten mittels der Ultrazentrifuge. Arzneimittel-Forsch. 16, 109 (1966).Google Scholar
  7. Spratt, J. L., and G. T. Okita: Protein binding of radioactive digitoxin. J. Pharmacol. exp. Ther. 124, 109 (1958).Google Scholar

Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • Klaus Kuschinsky
    • 1
  1. 1.Institut für Pharmakologie, Universität KielGermany

Personalised recommendations