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Isolation and characterization of the 80000 dalton Plasmodium falciparum merozoite surface antigen

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Abstract

The 80 000 dalton merozoite surface protein, derived from the 185 000–195 000 dalton schizont precursor, was isolated from detergent extracts of naturally-released merozoites using chromatographic procedures. A rabbit antiserum raised against this antigen was used for characterizing the 80 000 dalton fragment. The antiserum did not inhibit merozoite invasion or parasite growth in vitro, suggesting that this merozoite surface antigen is not directly involved in the invasion process of the merozoite into the host erythrocyte.

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References

  • Aebersold RH, Teplow DB, Hood LE, Kent SBH (1986) Electroblotting onto activated glass. J Biol Chem 261:4229–4238

    Google Scholar 

  • Anderson DJ, Adams PH, Hamilton MS, Alexander NJ (1983) Antisperm antibodies in mouse vasectomy sera with embryonal teratocarcinoma. J Immunol 131:2908–2912

    Google Scholar 

  • Cheung A, Shaw AR, Leban J, Perrin LH (1985) Cloning and expression in Escherichia coli of a surface antigen of Plasmodium falciparum merozoites. EMBO J 4:1007–1012

    Google Scholar 

  • Desjardins RE, Canfield CJ, Haynes JD, Chulay JD (1979) Quantitative assessment of antimalarial activity in vitro by a semi-automated microdilution technique. Antimicrob Agents Chemother 16:710–718

    Google Scholar 

  • Fairbanks G, Steck TL, Wallach DFH (1971) Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617

    Google Scholar 

  • Freemann RR, Holder AA (1983) Surface antigens of malaria merozoites. A high molecular weight precursor is processed to an 83 000 mol wt form expressed on the surface of Plasmodium falciparum merozoites. J Exp Med 158:1647–1653

    Google Scholar 

  • Hall R, Hyde JE, Goman M, Simons DL, Hope IA, Mackay M, Scaife J, Merkli B, Richle R, Stocker J (1984) Major surface antigen gene of a human malaria parasite cloned and expressed in bacteria. Nature 311:379–382

    Google Scholar 

  • Heidrich HG, Strych W, Mrema JEK (1983) Identification of surface and internal antigens from spontaneously released Plasmodium falciparum merozoites by radio-iodination and metabolic labelling. Z Parasitenkd 69:715–725

    Google Scholar 

  • Heidrich HG, Strych W, Prehm P (1984) Spontaneously released Plasmodium falciparum merozoites from culture possess glycoproteins. Z Parasitenkd 70:747–751

    Google Scholar 

  • Heidrich HG, Matzner M, Miettinen-Baumann A, Strych W (1986) Immunoelectron microscopy shows that the 80000-dalton antigen of Plasmodium falciparum is localized in the surface coat. Z Parasitenkd 72:681–683

    Google Scholar 

  • Holder AA, Freeman RR (1982) Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody. J Exp Med 156:1528–1538

    Google Scholar 

  • Holder AA, Freeman RR (1984) The three major antigens on the surface of Plasmodium falciparum merozoites are derived from a single high molecular weight precursor. J Exp Med 160:624–629

    Google Scholar 

  • Holder AA, Lockyer MJ, Odink KG, Dandhu JS, Riveros-Moreno V, Nickolls SC, Hillman Y, Davey LS, Tizard MLV, Schwarz RT, Freeman RR (1985) Primary structure of the precursor of the three major surface antigens of Plasmodium falciparum merozoites. Nature 317:270–273

    Google Scholar 

  • Jensen JB (1977) Concentration from continuous culture of erythrocytes infected with trophozoites and schizonts of Plasmodium falciparum. Am J Trop Med Hyg 27:1274–1276

    Google Scholar 

  • Jepsen S (1983) Inhibition of in vitro growth of Plasmodium falciparum by purified antimalarial human IgG antibodies. Isolation of target antigens from culture supernatants. Scand J Immunol 18:567–571

    Google Scholar 

  • Jepsen S, Andersen BJ (1981) Immunosorbent isolation of antigens from the culture medium of in vitro cultivated P. falciparum. Acta Pathol Microbiol Immunol Scand (C) 89:99–103

    Google Scholar 

  • Lambros C, Vanderberg JW (1979) Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol 65:418–420

    Google Scholar 

  • Lottspeich F (1985) Microscale isocratic separation of phenyltiohydantoin amino acid derivatives. J Chromatogr 326:321–327

    Google Scholar 

  • Lyon JA, Geller RH, Haynes JD, Chulay JD, Weber JL (1986) Epitope map and processing scheme for the 195000-dalton surface glycoprotein of Plasmodium falciparum merozoites deduced from cloned overlapping segments of the gene. Proc Natl Acad Sci USA 83:2989–2993

    Google Scholar 

  • Mackay M, Goman M, Bone N, Hyde JE, Scaife J, Certa U, Stunnenberg H, Bujard H (1985) Polymorphism of the precursor for the major surface antigens of Plasmodium falciparum merozoites: studies at the genetic level. EMBO J 13:3823–3829

    Google Scholar 

  • McBride JS, Heidrich HG (1987) Fragments of the polymorphic Mr 185000 glycoprotein from the surface of isolated P. falciparum merozoites form an antigenic complex. Mol Biochem Parasitol 23:71–84

    Google Scholar 

  • McBride JS, Newbold CI, Anand R (1985) Polymorphism of a high molecular weight schizont antigen of the human malaria parasite Plasmodium falciparum. J Exp Med 161:160–180

    Google Scholar 

  • Mrema JEK, Langreth SG, Jost RC, Rieckmann KH, Heidrich HG (1982) Plasmodium falciparum: isolation and purification of spantaneously released merozoites by nylon membrane sieves. Exp Parasitol 54:285–295

    Google Scholar 

  • Pasvol G, Wilson RJ, Smalley ME, Brown J (1978) Separation of viable schizont-infected red blood cells of Plasmodium falciparum from human blood. Ann Trop Med Parasitol 72:87–88

    Google Scholar 

  • Perrin LH, Dayal R (1982) Immunity to asexual erythrocytic stages of Plasmodium falciparum: role of defined antigens in the humoral response. Immunol Rev 61:245–269

    Google Scholar 

  • Perrin LH, Ramirez E, Er-Hsiang L, Lambert PH (1980) Plasmodium falciparum: characterization of defined antigens by monoclonal antibodies. Clin Exp Immunol 41:91–96

    Google Scholar 

  • Perrin LH, Loche M, Dedet JP, Roussilhon C, Fandeur T (1984a) Immunization against Plasmodium falciparum asexual blood stages using soluble antigens. Clin Exp Immunol 56:67–72

    Google Scholar 

  • Perrin LH, Merkli B, Loche M, Chizzolini C, Smart S, Richle R (1984b) Antimalarial immunity in Saimiri monkeys: immunization with surface components of asexual blood stages. J Exp Med 160:441–451

    Google Scholar 

  • Reese RT, Langreth SG, Trager W (1979) Isolation of stages of the human parasite Plasmodium falciparum from culture and from animal blood. Bull WHO 57:53–61

    Google Scholar 

  • Shamansky LM, Liu HY, Hager LP, Ristic M (1985) Purification and characterization of culture-derived exo-antigens of P. falciparum. Mol Biochem Parasitol 17:299–310

    Google Scholar 

  • Thelu J, Ambroise-Thomas P, Chumpitazi B, Kupka P (1985) Purification and immunochemical study of Plasmodium falciparum exoantigens. J Parasitol 71:542–546

    Google Scholar 

  • Towbien H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    Google Scholar 

  • Trager W, Jensen JD (1976) Human malaria parasites in continuous culture. Science 193:673–675

    Google Scholar 

  • Vernot-Hernandez JP, Heidrich HG (1985) The relationship to knobs of the 92000 dalton protein specific for knobby strains of Plasmodium falciparum. Z Parasitenkd 71:41–51

    Google Scholar 

  • Wray W, Boulikas T, Wray VP, Hancock R (1981) Silver staining of proteins in polyacrylamide gels. Anal Biochem 118:197–203

    Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institut für Biochemie, D-8033, Martinsried bei München, Federal Republic of Germany

    W. Strych, A. Miettinen-Baumann, F. Lottspeich & H. -G. Heidrich

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  1. W. Strych
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  2. A. Miettinen-Baumann
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  3. F. Lottspeich
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  4. H. -G. Heidrich
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Strych, W., Miettinen-Baumann, A., Lottspeich, F. et al. Isolation and characterization of the 80000 dalton Plasmodium falciparum merozoite surface antigen. Parasitol Res 73, 435–441 (1987). https://doi.org/10.1007/BF00538201

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  • DOI: https://doi.org/10.1007/BF00538201

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