Abstract
Significant differences were found in the ability of resident mouse peritoneal macrophages to ingest amastigote and promastigote forms of Leishmania mexicana amazonensis. Differences in the association index of the parasites to the macrophages were also found between infective and non-infective promastigotes. Evidence was obtained suggesting that the macrophage receptor, which recognizes mannose-6-phosphate-containing units found in lysosomal enzymes, is involved in the association with the macrophage of promastigotes, but not of amastigotes. Addition of mannose-6-phosphate, its structural analogue fructose-1-phosphate, Hansenula holstii phosphomannan or the mannose-6-phosphate-containing lysosomal enzyme α-D-mannosidase to the interaction medium, markedly inhibits the association of the parasites with macrophages.
Similar content being viewed by others
References
Adler S (1963) Differentiation of Leishmania braziliensis from L. mexicana and L. tropica. Rev Inst Salubr Enferm Trop (Mexico) 23:139–150
Andrade ZA, Reed SG, Roters SB, Sadigursky M (1984) Immunopathology of Experimental Cutaneous Leishmaniasis. Am J Pathol 114:137–148
Blackwell JM, Ezekowitz RAB, Roberts MB, Channon JY, Sim RB, Gordon S (1985) Macrophage complement and lectin-like receptors bind Leishmania in the absence of serum. J Exp Med 162:324–331
Bray RS (1983) Leishmania mexicana amazonensis: attachment and uptake of promastigotes to and by macrophages in vitro. J Protozool 30:314–322
Chang KP (1978) Leishmania infection of human skin fibroblasts in vitro: absence of phagolysosomal fusion after induced phagocytosis of promastigotes, and their intracellular transformation. Am J Trop Med Hyg 21:1084–1096
Chang KP (1981) Leishmania donovani-macrophage binding mediated by surface glycoproteins/antigens: characterization in vitro by a radioisotopic assay. Mol Biochem Parasitol 4:67–76
Chang KP, Dwyer DM (1978) Leishmania donovani-hamster macrophage interactions in vitro: cell entry intracellular survival and multiplication of amastigotes. J Exp Med 147:515–530
Channon JY, Roberts MB, Blackwell JM (1984) A study of the differential respiratory burst activity elicited by promastigotes and amastigotes of Leishmania donovani in murine resident peritoneal. Immunology 53:345–355
Dedet JP, Ryter A, Vogt E, Hosli P, Pereira da Silva L (1983) Uptake and killing of Leishmania mexicana amazonensis amastigotes by human skin fibroblasts. Ann Trop Med Parasitol 77:35–44
Dwyer DM, Gottlieb M (1983) The surface membrane chemistry of Leishmania: its possible role in parasite sequestration and survival. J Cell Biochem 23:35–45
Ebert F, Buse E, Mühlpfordt H (1979) In vitro light and electron microscope studies on different virulent promastigotes of Leishmania donovani in hamster peritoneal macrophages. Z Parsitenkd 59:31–41
Freeze HH (1985) Interaction of Dictyostelium discoideum lysosomal enzymes with the mammalian phosphomannosyl receptor. The importance of oligosaccharides which contain phosphodiesters. J Biol Chem 260:8857–8864
Freeze HH, Yeh RY, Miller AL (1983) Uptake of α-d-mannosidase and Β-d-glucosidase from Dictyostelium discoideum via the phosphohexosyl receptor on normal human fibroblasts. J Biol Chem 258:8928–8933
Giannini MS (1974) Effects of promastigote growth phase, frequency of subculture and host age on promastigote initiated infection with Leishmania donovani in the golden hamster. J Protozool 21:521–527
Glew RH, Czuczman MS, Diven WF, Berens RL, Pope HT, Katsoulis DE (1982) Partial purification and characterization of particulate acid phosphatase of Leishmania donovani promastigotes. Comp Biochem Physiol [B] 72:581–590
Gottlieb M, Dwyer DM (1981 a) Protozoan parasite of humans: surface membrane with externally disposed acid phosphatase. Science 212:939–941
Gottlieb M, Dwyer DM (1981 b) Leishmania donovani: surface membrane acid phosphatase activity of promastigotes. Exp Parasitol 52:117–128
Grimaldi G, CÔrte-Real S, de Pinho RT, Moriearty PL (1983) Interactions between Leishmania mexicana promastigotes and amastigotes and murineperitoneal macrophages in vitro. Mem Inst Oswaldo Cruz 78:135–146
Handman E, Goding JW (1985) The Leishmania receptor for macrophages is a lipid-containing glycoconjugate. EMBO J 4:329–336
Handman E, Jarvis HM, Mitchell GF (1984) Leishmania major: identification of stage-specific antigens and antigens shared by promastigotes and amastigotes. Parasite Immunology 6:223–233
Li YT (1967) Studies on the glycosidases in Jack bean meal. I. Isolation and properties of α-mannosidase. J Biol Chem 242:5474–5480
Mosser DM, Edelson PJ (1984) Activation of the alternative complement pathway by Leishmania promastigotes: parasite lysis and attachment to macrophages. J Immunol 132:1501–1505
Mosser DM, Edelson PJ (1985) The mouse macrophage receptor for C3bi (CR3) is a major mechanism in the phagocytosis of Leishmania promastigotes. J Immunol 135:2785–2789
Pimenta PFP, de Souza W (1983) Leishmania mexicana amazonensis: surface charge of amastigotes and promastigote forms. Exp Parasitol 56:194–206
Pimenta PFP, de Souza W (1986) Cytochemical localization of acid phosphatase in Leishmania mexicana amazonensis. J Submicrosc Cytol 18:127–132
Remaley AT, Das S, Campbell PI, Larocca GM, Pope MT, Glew RH (1985) Characterization of Leishmania donovani acid phosphatases. J Biol Chem 260:880–886
Sadick MD, Raff HV (1985) Differences in expression and exposure of promastigote and amastigote membrane molecules in Leishmania tropica. Infect Immun 47:395–400
Saraiva EMB, Pimenta PFP, Pereira MEA, de Souza W (1983) Isolation and purification of amastigotes of Leishmania mexicana amazonensis by a gradient of Metrizamide. J Parasitol 69:627–629
Saraiva EMB, Andrade AFB, Pereira MEA (1986) Cell surface carbohydrate differences between infective and non-infective forms of Leishmania mexicana amazonensis. Eur J Cell Biol 40:219–225
Schwartzman JD, Pearson RD (1985) The interaction of Leishmania donovani promastigotes and human fibroblasts in vitro. Am J Trop Med Hyg 34:850–855
Shepherd VL, Freeze HH, Miller AL, Stahl PD (1984) Identification of mannose-6-phosphate receptors in rabbit alveolar macrophages. J Biol Chem 259:2257–2261
Sly WS, Fischer HD (1982) The phosphomannosyl recognition system for intracellular and intercellular transport of lysosomal enzymes. J Cell Biochem 18:67–85
Warren L (1960) Metabolism of Schizotrypanum cruzi, Chagas. I. Effect of culture age and substrate concentration on respiratory rate. J Parasitol 45:529–539
Wilson ME, Pearson RD (1985) Role for mannose receptors in the ingestion of Leishmania donovani by human macrophages. Clin Res 33:423a
Wyler DJ, Sypek JP, McDonald JA (1985) In vitro parasitemonocyte interactions in human leishmaniasis: possible role of fibronectin in parasite attachment. Infect Immun 49:305–311
Zuckerman A (1953) Initial reaction to the subcutaneous inoculation of cultures of Leishmania tropica in the hamster. Acta Med Orient 12:238–240
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Saraiva, E.M.B., Andrade, A.F.B. & de Souza, W. Involvement of the macrophage mannose-6-phosphate receptor in the recognition of Leishmania mexicana amazonensis . Parasitol Res 73, 411–416 (1987). https://doi.org/10.1007/BF00538197
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00538197