Die Spezifität der Nitratreduktase-Induktion bei Bac. Cereus

  • Eberhard Hackenthal
  • Renate Hackenthal
Article

Zusammenfassung

Die Induktion der Nitratreduktase bei Bacillus Cereus durch verschiedene monovalente anorganische Anionen wurde untersucht. Anaerob in komplexem Milieu wachsende Zellen wurden zu verschiedenen Zeiten nach Zusatz des betreffenden Anions geerntet und in daraus hergestellten Zellsuspensionen und zellfreien Extrakten die Nitratreduktion gemessen. Etwa gleichwertig als Induktoren sind Nitrat, Oxycyanid und Azid, dann folgen in der Reihe abnehmender Wirksamkeit Jodid, Perchlorat, Nitrit, Rhodanid, Fluoroborat und Perrhenat. Nicht induzierend wurden Cyanid, Hydroxylamin und Ammoniumsulfaminat gefunden. Es besteht keine Korrelation zwischen Induktionsfähigkeit und Enzymaffinität. Jodid und Fluoroborat haben weder in vitro noch in vivo Enzymaffinität.

Summary

The induction of biosynthesis of nitrate reductase by some anorganic anions structurally related to nitrate has been studied by measuring the nitrate reduction of cell suspensions and cellfree extracts obtained from anaerobically growing cells of Bac. cereus at various time intervals after the addition of the anion tested. The biosynthesis of nitrate reductase was induced by nitrate, oxycyanide, azide, iodide, perchlorate, nitrite, thiocyanate, fluoroborate, and perrhenate in decreasing order of effectiveness. Nitrate reductase was not induced by cyanide, hydroxylamine and ammonium sulfaminate. The potency of the anions as inducers is not correlated to their enzyme affinity. Two of the anions, iodide and fluoroborate, are inducers and have no affinity to nitrate reductase in intact cells and cellfree extracts.

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Copyright information

© Springer-Verlag 1966

Authors and Affiliations

  • Eberhard Hackenthal
    • 1
  • Renate Hackenthal
    • 1
  1. 1.Pharmakologisches Institut der Universität HeidelbergGermany

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