Zusammenfassung
Die Induktion der Nitratreduktase bei Bacillus Cereus durch verschiedene monovalente anorganische Anionen wurde untersucht. Anaerob in komplexem Milieu wachsende Zellen wurden zu verschiedenen Zeiten nach Zusatz des betreffenden Anions geerntet und in daraus hergestellten Zellsuspensionen und zellfreien Extrakten die Nitratreduktion gemessen. Etwa gleichwertig als Induktoren sind Nitrat, Oxycyanid und Azid, dann folgen in der Reihe abnehmender Wirksamkeit Jodid, Perchlorat, Nitrit, Rhodanid, Fluoroborat und Perrhenat. Nicht induzierend wurden Cyanid, Hydroxylamin und Ammoniumsulfaminat gefunden. Es besteht keine Korrelation zwischen Induktionsfähigkeit und Enzymaffinität. Jodid und Fluoroborat haben weder in vitro noch in vivo Enzymaffinität.
Summary
The induction of biosynthesis of nitrate reductase by some anorganic anions structurally related to nitrate has been studied by measuring the nitrate reduction of cell suspensions and cellfree extracts obtained from anaerobically growing cells of Bac. cereus at various time intervals after the addition of the anion tested. The biosynthesis of nitrate reductase was induced by nitrate, oxycyanide, azide, iodide, perchlorate, nitrite, thiocyanate, fluoroborate, and perrhenate in decreasing order of effectiveness. Nitrate reductase was not induced by cyanide, hydroxylamine and ammonium sulfaminate. The potency of the anions as inducers is not correlated to their enzyme affinity. Two of the anions, iodide and fluoroborate, are inducers and have no affinity to nitrate reductase in intact cells and cellfree extracts.
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Literatur
Dixon, M., and E. C. Webb: The Enzymes, 2nd ed. p. 501. London: Longmans, Green and Co. Ltd. 1964.
Farkas-Himsley, H., and M. Artman: Studies on nitrate reduction by E. coli. J. Bact. 74, 690–692 (1957).
Goldstein, D. B.: Induction of cholinesterase biosynthesis in Pseudomonas fluorescens. J. Bact. 78, 695–702 (1951).
Gross, S. R., and E. L. Tatum: Structural specifity of inducers of procatechuic acid oxidase synthesis in Neurospora. Science 122, 1141 P (1955).
Hackenthal, E.: Die Reduktion von Perchlorat durch Bakterien. II. Die Identität von Nitratreduktase und Perchlorat reduzierendem Enzym aus Bac. Cereus. Biochem. Pharmacol. 14, 1313–1324 (1965a).
-- Die Hemmung der Nitratreduktase aus Bac. Cereus durch verschiedene Anionen. Hoppe-Seylers Z. physiol. Chem. (im Druck) (1965b).
——, u. R. Hackenthal: Die Nitratreduktion durch zellfreie Extrakte aus Bac. Cereus. Biochem. biophys. Acta (Amst.) 107, 189–202 (1965).
Halvorson, H. O.: The induced synthesis of proteins. Advances in Enzymol. 22, 99 bis 156 (1960).
Lester, G.: The β-galactosidase of lactose mutants in Escherichia coli K 12. Arch. Biochem. 40, 390–401 (1952).
Mandelstam, J., and J. Yudkin: Studies in biochemical adaptation. Some aspects of galactozymase production by yeast in relation to the “Mass Action” theorie of enzyme adaptation. Biochem. J. 51, 663–693 (1952).
Monod, J., G. Cohen-Bazire et M. Cohn: Sur la biosynthèse de la galactosidase (Lactase) chez Escherichia coli. La specifité de l'induction. Biochim. biophys. Acta (Amst.) 7, 585–599 (1951).
Nason, A.: Symposium on metabolism of inorganic compounds. II. Enzymatic pathways of nitrate, nitrite and hydroxylamine metabolisms. Bact. Rev. 26, 16–41 (1962).
Nicholas, D. J. D.: The metabolism of inorganic nitrogen and its compounds in micro-organisms. Biol. Rev. 38, 530–568 (1963).
Pardee, A. B.: An inducible mechanism for the accumulation of melibiose in Escherichia coli. J. Bact. 73, 376–382 (1957).
—— The synthesis of enzymes. In: The Bacteria. Vol. III, p. 577. (I. C. Gunsalus and R. Y. Stannier, eds.) New York, London: Academic Press 1962.
Perrin, D., F. Jacob et J. Monod: Biosynthèse induite d'une protéine génétiquement modifiée, ne presentant pas d'affinité pour l'inducteur. C. R. Acad. Sci. (Paris) 251, 155–157 (1960).
Pichinoty, F., et L. D'Ornano: Biosynthèse induite de la nitrate-reductase par les cellules non proliferantes d'Aerobacter Aerogenes. C. R. Acad. Sci. (Paris) 252, 793–795 (1961a).
—— —— Inhibition by oxygen of biosynthesis and activity of nitrate reductase in Aerobacter Aerogenes. Nature (Lond.) 191, 879–881 (1961b).
Pollock, M. R.: Induced formation of enzymes. In: The Enzymes, 2nd ed., p. 619 to 680. (P. D. Boyer, H. Lardy, and K. Myrbäck, eds.) New York, London: Academic Press 1959.
Rickenberg, A. V.: Role of inducers in the induced synthesis of β-galactosidase in Escherichia coli. Nature (Lond.) 185, 240–241 (1960).
——, and G. Lester: The preferential synthesis of β-galactosidase in Escherichia coli. J. gen. Microbiol. 13, 279–296 (1955).
Tanigushi, S., and E. Itagaki: Nitrate reductase of respiration type from E. coli. I. Solubilization and purification from the particulate system with characterization as a metalloprotein. Biochim. biophys. Acta (Amst.) 44, 263–273 (1960).
Wainwright, S. D.: Formation of a bacterial adaptive enzyme system in the absence of substrate: Production of nitratase by Bact. coli without nitrate. Brit. J. exp. Path. 31, 495–506 (1950).
——, and A. Nevill: The influence of depletion of nitrogenous reserves upon the phenomenon of induced enzyme biosynthesis in cells of Escherichia coli. J. gen. Microbiol. 14, 47–56 (1956).
Yudkin, J.: Enzyme variation in microorganisms. Biol. Rev. 13, 93–106 (1938).
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Diese Arbeit wurde mit finanzieller Unterstützung der Deutschen Forschungsgemeinschaft sowie der Strebelstiftung für Krebs- und Scharlachforschung durchgeführt.
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Hackenthal, E., Hackenthal, R. Die Spezifität der Nitratreduktase-Induktion bei Bac. Cereus. Naunyn-Schmiedebergs Arch. Pharmak. u. Exp. Path. 254, 56–66 (1966). https://doi.org/10.1007/BF00537822
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DOI: https://doi.org/10.1007/BF00537822