Zusammenfassung
Die direkte Behandlung von Organkulturen embryonalen und erwachsenen Lungengewebes von Mäusen mit Urethan ruft keine Tumoren hervor; dagegen führt die subcutane Injektion von 100–120 mg Urethan in der zweiten Hälfte der Schwangerschaft zu präadenomatösen Wucherungen und zahlreichen Adenomen. Nach 120 mg Urethan während der letzten 10 Tage der Schwangerschaft erreichte die Zahl der Adenome in Organkulturen von explantierten Lungen der Embryonen 66,6%. Auf Grund des Vergleiches der Ergebnisse der transplacentalen und unmittelbaren Einwirkung von Urethan neigt, der Verfasser der Meinung zu, daß die blastomogene Wirkung des Urethans eine indirekte ist.
Summary
The direct treatment of organ cultures of embryonic and adult lung tissue of mice with urethane did not induce tumours, but the subcutaneous introduction of 100–120 mg of urethane in the second part of pregnancy induced preadenomatous growth and numerous adenomas in the explants of the lungs of the embryos. After 120 mg of urethane during the last 10 days of pregnancy the number of lung adenomas in the organ cultures reached 66,6%. By comparison of the results of transplacental and immediate introduction of urethane the author inclines to the opinion that the blastomogenic action of urethane is indirect.
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References
Adilgireeva, R. H.: Explantation of normal and neoplastic lung tissues. Bull exp. Biol. Med. 57 (4), 115–118 (1964) [Russ.].
Berenblum, J.: In vivo test for a carcinogenic metabolite in plasma of animals given injections of ethyl carbamate (Urethane). Cancer Res. 20, (1), 38 (1960).
Boyland, E.: The biochemical mechanism of the action of urethane. Int. confer. lung tumors in animals. Abstracts. Perugia 57 (1965).
Griciute, L. A.: Morphology and histogenesis of lung adenomas after injection of urethane. Arch. Path. 19 (4), 22–31 (1957) [Russ.].
Klein, M.: Lung adenomas in offspring following injection of pregnant mice with urethane. Cancer Res. 12, (4), 275 (1952).
Kolesnichenko, T. S.: Development of adenomas in organ culture of mouse embryonic lung tissue. Vop. Onkol. 12 (12), 39–47 (1966). [Russ.].
Larsen, C. B.: Pulmonary tumor induction by transplacental exposure to urethane. J. nat. Cancer Inst. 8, 63–70 (1947).
—: Pulmonary tumor induction with alkylated urethanes. J. nat. Cancer Inst. 9, 35 (1948).
Laws, J. O., and A. Flaks: Pulmonary adenomata induced by carcinogen treatment in organ culture. Brit. J. Cancer 20 (3), 550–554 (1966).
Malmgren, R. A., and E. A. Saxen: Studies of carcinogenic effect of urethane on transplantation of the lungs in strain A mice. J. nat. Cancer Inst. 14 (2), 411–423 (1953).
Rogers, S.: Studies of the mechanism of action of urethane in initiating pulmonary adenomas in mice. J. exp. Med. 105 (4), 279–306 (1957).
Shabad, L. M.: Les tumeurs primitives du poumon chez les souris badigeonnées au goudron. C. R. Soc. Biol. (Paris), 1383–1384, 1497–1498, 1555–1551 (1928).
—: Studien über primäre Lungengeschwülste bei Mäusen und ihr Verhalten zum Steinkohlenteer als cancerogenen Factor. Z. Krebsforsch. 30 (1), 24–59 (1929).
Shabad, L. M.: Precancer. Experimental-morphological aspects. Medizina (Moskwa) 1967 [Russ.].
Smith, W., and P. J. Rous: The neoplastic potentialities of mouse embryo tissues. J. exp. Med. 5, 529–554 (1948).
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Shabad, L.M. Organ culture of lung tissue, as a method of the study of lung tumours and the blastomogenic action of substances that can induce them. Z Krebs-forsch 70, 198–203 (1968). https://doi.org/10.1007/BF00536992
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DOI: https://doi.org/10.1007/BF00536992