Abstract
The effect of the deacetylated (amine) metabolite of diamphenethide (10 Μg/ml) on the tegumental surface of Fasciola hepatica over a 24 h period in vitro has been determined by scanning electron microscopy. Blebbing begins around the oral sucker after 3 h and then passes backwards along the body, reaching the ventral sucker and midbody by 6 h, and finally the posterior end of the body (by 12 h). Initially, the blebs are small, the tegument surrounding the spines is swollen and the tegument generally has a smooth, swollen appearance. This submerges the spines below the body surface. At higher magnification the surface is seen to bear microvillous-like projections in addition to the blebs and surface pitting is deeper than normal. Later on, the blebs increase in size and burst, causing lesions and loss of spines. Lesions begin to appear on the oral cone and ventral sucker after 6 h, in the midbody by 12 h and on the dorsal surface of the posterior region after 24 h. By this time the damage is extensive: around the oral and ventral suckers, and over large areas of the oral cone and midbody region the tegument has been stripped off to expose the basal lamina beneath. The dorsal surface of the fluke is consistently more severely affected than the ventral surface.
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References
Andrews P, Thomas H, Weber H (1980) The in vitro uptake of 14C-praziquantel by cestodes, trematodes, and a nematode. J Parasitol 66:920–925
Becker B, Mehlhorn H, Andrews P, Thomas H, Eckert J (1980) Light and electron microscopic studies on the effect of praziquantel on Schistosoma mansoni, Dicrocoelium dendriticum, and Fasciola hepatica (Trematoda) in vitro. Z Parasitenkd 63:113–128
Becker B, Mehlhorn H, Andrews P, Thomas H (1981) Ultrastructural investigations on the effect of praziquantel on the tegument of five species of cestodes. Z Parasitenkd 64:257–267
Bennett CE, Hughes DL, Harness E (1980) Fasciola hepatica: changes in tegument during killing of adult flukes surgically transferred to sensitized rats. Parasite Immunol 2:39–55
Bricker CS, Depenbusch JW, Bennett JL, Thompson DP (1983) The relationship between tegumental disruption and muscle contraction in Schistosoma mansoni exposed to various compounds. Z Parasitenkd 69:61–71
Coles GC (1976) The inactivity of diamphenethide against liver fluke in certain species of mammals. Res Vet Sci 20:110–111
Conder GA, Marchiondo AA, Andersen FL (1981) Effect of praziquantel on adult Echinococcus granulosus in vitro: scanning electron microscopy. Z Parasitenkd 66:191–199
Dawes B (1966) Experimental fascioliasis: some effects on Fasciola hepatica of treatment of rat hosts with bithionol (“Actamer”). Helminthologia 7:297–307
Dawes B (1967) Experimental fascioliasis in small mammals: apparent effects of bithionol on Fasciola hepatica in rats. Helminthologia 8:97–101
Dawes B (1968) Further evidence on the effect of bithionol (“Actamer”) on Fasciola hepatica. Wiad Parazytol 14:575–577
Fairweather I, Holmes SD, Threadgold LT (1984) Fasciola hepatica: motility response to fasciolicides in vitro. Exp Parasitol 57:209–224
Fairweather I, Anderson HR, Threadgold LT (1986) Fasciola hepatica: tegumental changes induced in vitro by the deacetylated (amine) metabolite of diamphenethide. Exp Parasitol (in press)
Gönnert R, Andrews P (1977) Praziquantel, a new broad-spectrum antischistosomal agent. Z Parasitenkd 52:129–150
Gusel'nikova LM (1974) The effect of certain fasciolicides on the tegument of Fasciola hepatica implanted under the skin on the back of white rats. Med Parazitol (Mosk) 43:214–217
Hanna REB (1980) Fasciola hepatica: an immunofluorescent study of antigenic changes in the tegument during development in the rat and the sheep. Exp Parasitol 50:155–170
Hillman GR, Gibier WB, Anderson JB (1977) Comparative effects of hycanthone in Schistosoma mansoni and Schistosoma japonicum. Am J Trop Med Hyg 26:238–242
Isseroff H, Read CP (1969) Studies on membrane transport — VI. Absorption of amino acids by fascioliid trematodes. Comp Biochem Physiol 30:1153–1160
Isseroff H, Read CP (1974) Studies on membrane transport — VIII. Absorption of monosaccharides by Fasciola hepatica. Comp Biochem Physiol [A] 47:141–152
Kendall SB, Parfitt JW (1973) The effect of diamphenethide on Fasciola hepatica at different stages of development. Res Vet Sci 15:37–40
Kohn A, López-Alvarez ML, Katz N (1982) Transmission and scanning electron microscopical studies in the tegument of male Schistosoma mansoni after oxamniquine treatment. Ann Parasitol Hum Comp 57:285–291
Rew RS, Fetterer RH (1984) Effects of diamfenetide on metabolic and excretory functions of Fasciola hepatica in vitro. Comp Biochem Physiol [C] 79:353–355
Rew RS, Fetterer RH, Martin TC (1983) Fasciola hepatica: effects of diamfenetide free amine on in vitro physiology, biochemistry, and morphology. Exp Parasitol 55:159–167
Rogan MT, Threadgold LT (1984) Fasciola hepatica: tegumental alterations as a consequence of lectin binding. Exp Parasitol 57:248–260
Rowlands D ap T (1973) Diamphenethide — a drug offering a fresh approach to the treatment of liver fluke disease in sheep. Pestic Sci 4:883–889
Shaw MK, Erasmus DA (1983a) Schistosoma mansoni: the effects of a subcurative dose of praziquantel on the ultrastructure of worms in vivo. Z Parasitenkd 69:73–90
Shaw MK, Erasmus DA (1983b) Schistosoma mansoni: doserelated tegumental surface changes after in vivo treatment with praziquantel. Z Parasitenkd 69:643–653
Stammers BM (1975a) The effects of rafoxanide and nitroxynil on the survival, growth and morphology of Fasciola hepatica in rabbits. Z Parasitenkd 46:153–161
Stammers BM (1975b) Disruption of spermatogenesis in Fasciola hepatica by some anthelmintics. Z Parasitenkd 47:145–150
Thorsell W, Björkman N (1966) In vitro studies on the effect of hexachlorophene and its dimethylether on the liver fluke, Fasciola hepatica L. Z Parasitenkd 28:116–124
Threadgold LT (1985) Fasciola hepatica: interaction of the tegument with poly-L-lysine and enzymes. Exp Parasitol 59:222–230
Threadgold LT, Brennan G (1978) Fasciola hepatica: basal infolds and associated vacuoles of the tegument. Exp Parasitol 46:300–316
Verheyen A, Vanparijs O, Borgers M, Thienpont D (1978) Scanning electron microscopic observations of Cysticercus fasciolaris (= Taenia taeniaeformis) after treatment of mice with mebendazole. J Parasitol 64:411–425
Verheyen A, Vanparijs O, Lauwers H, Thienpont D (1980) The influence of closantel administration to sheep on the ultrastructure of the adult liver fluke Fasciola hepatica L. In: Van den Bossche M (ed) The Host-Invader Interplay. Elsevier/North-Holland Biomedical Press, Amsterdam New York Oxford, pp 705–708
Verheyen A, Vanparijs O, Van den Broeck C, Lauwers H, Thienpont D (1982) Progressive differences in drug-susceptibility of the adult liver fluke Fasciola hepatica L. Parasitology 84:li
Voge M, Bueding E (1980) Schistosoma mansoni: tegumental surface alterations induced by subcurative doses of the schistosomicide amoscanate. Exp Parasitol 50:251–259
Xiao S, Friedman PA, Catto BA, Webster LT Jnr (1984) Praziquantel-induced vesicle formation in the tegument of male Schistosoma mansoni is calcium dependent. J Parasitol 70:177–179
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Fairweather, I., Anderson, H.R. & Baldwin, T.M.A. Fasciola hepatica: tegumental surface alterations following treatment in vitro with the deacetylated (amine) metabolite of diamphenethide. Parasitol Res 73, 99–106 (1987). https://doi.org/10.1007/BF00536464
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DOI: https://doi.org/10.1007/BF00536464