Abstract
A method is described for the isolation of pure, chemically intact sheaths of blood microfilariae of Litomosoides carinii. Microfilariae were isolated according to standard techniques. Exsheathment was performed by freezing-thawingshaking procedures, repeated 5–10 times, i.e., larvae were frozen in liquid nitrogen, thawed at room temperature, and shaken vigorously for 5 s. Exsheathment rates were about 50%. Sheaths were separated from ensheathed and exsheathed microfilariae and microfilariae fragments by filtration through a polycarbonate filter (2 Μm pore size). The achievable yield (about 15% of the sheaths of a batch of microfilariae) was approximately 1 Μg of sheaths per 106 microfilariae.
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Chandrashekar R, Rao UR, Rajasekaria GR, Subrahmanyam D (1984) Separation of viable microfilariae free from blood cells on percoll gradients. J Helminthol 58:69–70
Devaney E, Howells RE (1979) The exsheathment of Brugia pahangi microfilariae under controlled conditions in vitro. Ann Trop Med Parasitol 73:227–233
LÄmmler G, Saupe E, Herzog H (1968) Infektionsversuche mit der Baumwollrattenfilarie Litomosoides carinii bei Mastomys natalensis (Smith, 1834). Z Parasitenkd 32:281–290
Schraermeyer U, Peters W, Zahner H (1987) Formation of a peripheral layer of the sheath of microfilariae of Litomosoides carinii and Brugia malayi by the uterus. Parasitol Res 73:557–564
Srivastava AK (1985) A method for isolation and purification of the sheath of microfilariae of Brugia malayi. J Parasitol 71:257–258
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Supported by the Deutsche Forschungsgemeinschaft
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Bardehle, G., Klonisch, T., Schott, H.H. et al. Isolation of pure sheaths of Litomosoides carinii microfilariae. Parasitol Res 74, 188–190 (1987). https://doi.org/10.1007/BF00536032
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DOI: https://doi.org/10.1007/BF00536032