Biophysics of structure and mechanism

, Volume 1, Issue 3, pp 259–271 | Cite as

Flash photometric experiments on the photochemical cycle of bacteriorhodopsin

  • N. Dencher
  • M. Wilms


The photochemical reaction cycle of bacteriorhodopsin was investigated by means of flash photometric methods. Three different intermediates with absorption maxima at about 630 nm, 411 nm, and 646 nm could be detected. Kinetic data of the occurrence of these intermediates were obtained from isolated purple membrane in different mediums and from intact halobacteria. An activation energy of 14.1±0.4 kcal·mol−1 and of about 19 kcal·mol−1 for the formation of bacteriorhodopsin 411 and of bacteriorhodopsin 565, resp., was calculated. pH-changes in the medium caused by the reaction cycle of bacteriorhodopsin were detected by use of the pH-indicator bromocresol green.

Key words

Bacteriorhodopsin Intermediate Products Reaction Kinetic pH-Changes 


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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • N. Dencher
    • 1
  • M. Wilms
    • 1
  1. 1.Kernforschungsanlage JülichInstitut für NeurobiologieJülichFed. Rep. of Germany

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