Biophysics of structure and mechanism

, Volume 6, Issue 2, pp 139–146 | Cite as

Flash-induced kinetic infrared spectroscopy applied to biochemical systems

  • F. Siebert
  • W. MÄntele
  • W. Kreutz
Article

Abstract

A flash photolysis apparatus with monitoring infrared beam is described allowing measurements of relative transmission changes of 10−3 in times of a few milliseconds. The investigation of the photodissociation of CO-myoglobin confirms the results obtained by static infrared difference spectroscopy. The application of our method to the rhodopsin/Meta II transition reveals signals which can tentatively be ascribed to the disappearance of the C=C-band of the protonated N-retinylidene Schiff base in rhodopsin. The developed method will be compared with other existing methods of kinetic vibronic spectroscopy such as kinetic resonance Raman spectroscopy and kinetic Fourier infrared spectroscopy.

Key words

Kinetic infrared spectroscopy CO-myoglobin Rhodopsin 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • F. Siebert
    • 1
  • W. MÄntele
    • 1
  • W. Kreutz
    • 1
  1. 1.Institut für Biophysik und Strahlenbiologie der UniversitÄt Freiburg im BreisgauFreiburg im BreisgauFederal Republic of Germany

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