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Fluorescence of blowfly metarhodopsin

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Abstract

The visual pigment of blowfly peripheral photoreceptors displays a marked red fluorescence when the pigment is in the metarhodopsin state as demonstrated in vivo by simultaneous measurements of transmission and fluorescence. The rhodopsin state is non-fluorescent.

It is argued that fluorescence offers a unique means to study visual pigment properties in completely intact living animals and, furthermore, provides the opportunity for studying the photoreceptor optics of visual waveguides. As an example the action of the pupil mechanism of blowfly visual sense cells on the fluorescence signal is demonstrated.

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References

  • Burghause FMHR (1979) Die strukturelle Spezialisierung des dorsalen Augenteils der Grillen (Orthoptera, Grylloidea). Zool Jahrb Abt Allg Zool Physiol Tiere 83: 502–525

    Google Scholar 

  • Cronin TW, Goldsmith TH (1981) Fluorescence of crayfish metarhodopsin studied in single rhabdoms. Biophys J 35: 653–664

    Google Scholar 

  • Cronin TW, Goldsmith TH (1982a) Photosensitivity spectrum of crayfish rhodopsin measured using fluorescence of metarhodopsin. J Gen Physiol 79: 313–332

    Google Scholar 

  • Cronin TW, Goldsmith TH (1982b) Quantum efficiency and photosensitivity of the rhodopsin ⇄ metarhodopsin conversion in crayfish photoreceptors. Photochem Photobiol 36: 447–454

    Google Scholar 

  • Franceschini N (1975) Sampling of the visual environment by the compound eye of the fly: Fundamentals and applications. In: Snyder AW, Menzel R (eds) Photoreceptor optics. Springer, Berlin Heidelberg New York, pp 98–125

    Google Scholar 

  • Franceschini N (1977) In vivo fluorescence of the rhabdomeres in an insect eye. Proc Int Union Physiol Sci XIII, 237. XXVIIth Int Congr Paris

  • Franceschini N (1983; in press) In vivo microspectrofluorimetry of visual pigments. In: Cosens DJ, Vincent-Prue (eds) The Biology of Photoreceptors, Symposia of the Society for Experimental Biology. Cambridge University Press, London

    Google Scholar 

  • Franceschini N, Kirschfeld K (1976) Le contrÔle automatique du flux lumineux dans l'oeil composé des Diptères. Propriétés spectrales, statiques et dynamiques du mécanisme. Biol Cybern 21: 181–203

    Google Scholar 

  • Franceschini N, Kirschfeld K, Minke B (1981a) Fluorescence of photoreceptor cells observed in vivo. Science 213: 1264–1267

    Google Scholar 

  • Franceschini N, Hardie R, Ribi W, Kirschfeld K (1981b) Sexual dimorphism in a photoreceptor. Nature (London) 291: 241–244

    Google Scholar 

  • Goldsmith TH (1972) The natural history of invertebrate visual pigments. In: Dartnall HJA (ed) Photochemistry of vision. Springer, Berlin Heidelberg New York (Handbook of sensory physiology, vol VII/1, pp 685–719)

    Google Scholar 

  • Guzzo AV, Pool GL (1968) Visual pigment fluorescence. Science 159: 312–314

    Google Scholar 

  • Hamdorf K (1979) The physiology of invertebrate visual pigments. In: Autrum H (ed) Invertebrate photoreceptors. Springer, Berlin Heidelberg New York (Handbook of sensory physiology, Vol VII/6A, pp 145–224)

    Google Scholar 

  • Hamdorf K, Schwemer J (1975) Photoregeneration and the adaptation process in insect photoreceptors. In: Snyder AW, Menzel R (eds) Photoreceptor optics. Springer, Berlin Heidelberg New York, pp 263–289

    Google Scholar 

  • Horridge GA, McLean M (1978) The dorsal eye of the mayfly Atalophlebia (Ephemeroptera). Proc R Soc London Ser B 200: 137–150

    Google Scholar 

  • Kirschfeld K, Franceschini N (1969) Ein Mechanismus zur Steuerung des Lichtflusses in den Rhabdomeren des Komplexauges von Musca. Kybernetik 6: 13–22

    Google Scholar 

  • Kruizinga B, Kamman R, Stavenga DG (1983) Laser induced visual pigment conversions in fly photoreceptors measured in vivo. Biophys Struct Mech 9: 301–309

    Google Scholar 

  • Liebman PA, Leigh RA (1969) Autofluorescence of visual receptors. Nature (London) 221: 1249–1251

    Google Scholar 

  • Packer L (ed) (1982) Visual pigments and purple membranes. In: Biomembranes. Academic Press, New York (Methods in enzymology, vol 81, Part H 1)

  • Stark WS, Ivanyshyn AM, Greenberg RM (1977) Sensitivity and photopigments of R1-6, a two-peaked photoreceptor, in Drosophila, Calliphora and Musca. J Comp Physiol 121: 289–305

    Google Scholar 

  • Stark WS, Stavenga DG, Kruizinga B (1979) Fly photoreceptor fluorescence is related to UV sensitivity. Nature (London) 280: 581–583

    Google Scholar 

  • Stavenga DG (1976) Fly visual pigments. Difference in visual pigments of blowfly and dronefly peripheral retinula cells. J Comp Physiol 111: 137–152

    Google Scholar 

  • Stavenga DG (1979) Pseudopupils of compound eyes. In: Autrum H (ed) Invertebrate photoreceptors. Springer, Berlin Heidelberg New York (Handbook of sensory of physiology, vol VII/6A, pp 357–439)

    Google Scholar 

  • Stavenga DG, Franceschini N (1981) Fly visual pigment states, rhodopsin R490, metarhodopsins M and M′, studied by transmission and fluorescence microspectrophotometry in vivo. Invest Ophthalmol Vis Sci (Suppl) 20: 111

    Google Scholar 

  • Stavenga DG, Kuiper JW (1977) Insect pupil mechanisms, I. On the pigment migration in the retinula cells of Hymenoptera (suborder Apocrita). J Comp Physiol 113: 55–72

    Google Scholar 

  • Stavenga DG, Schwemer J (1983; in press) Visual pigments of invertebrates. In: Ali MA (ed) Photoreception and vision in invertebrates. Plenum Press, New York

    Google Scholar 

  • Stavenga DG, Zantema A, Kuiper JW (1973) Rhodopsin processes and the function of the pupil mechanism in flies. In: Langer H (ed) Biochemistry and physiology of visual pigments. Springer, Berlin Heidelberg New York, pp 175–180

    Google Scholar 

  • Wolburg-Buchholz K (1977) The superposition eye of Cloeon dipterum: The organization of the lamina ganglionaris. Cell Tissue Res 177: 9–28

    Google Scholar 

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Stavenga, D.G. Fluorescence of blowfly metarhodopsin. Biophys. Struct. Mechanism 9, 309–317 (1983). https://doi.org/10.1007/BF00535666

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