Advertisement

Biophysics of structure and mechanism

, Volume 5, Issue 2–3, pp 163–174 | Cite as

Transduction in photoreceptors with bistable pigments: Intermediate processes

  • Baruch Minke
Article

Abstract

The prolonged depolarizing after potential (PDA) in the R1–6 receptors of the fly was used to isolate intermediate processes in phototransduction which are not manifested directly in the voltage response. It is first demonstrated that a pigment shift by light from metarhodopsin to rhodopsin in four species of the flies: Drosophila, Calliphora, Chrysomya and Musca induces an independent antagonistic process to the PDA, which is manifested in a strong inhibitory effect on PDA induction and is called the anti-PDA.

By using mutants of Drosophila the existence of processes underlying the PDA were examined. The norpAH52and the trp mutant were used in which the voltage response of the photoreceptors could be reversibly abolished by elavated temperature and long intense light respectively. It is shown that the excitatory process underlying the PDA could be induced and depressed in conditions that block the voltage response of the photoreceptors, thus indicating the existance of intermediate processes which link the pigment activation by light to the PDA voltage response.

Key words

PDA anti-PDA Drosophila mutants Phototransduction 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Cosens, D. J., Manning, A.: Abnormal electroretinogram from a Drosophila mutant. Nature 224, 285–287 (1969)Google Scholar
  2. Deland, M. C., Pak, W. L.: Reversibly temperature sensitive phototransduction mutant of Drosophila melanogaster. Nature 224, 184–186 (1973)Google Scholar
  3. Goldsmith, T. H., Bernard, G. D.: The visual system of insects. In: Physiology of insecta, 2nd Ed. Rockstein, M. (ed.). Vol. 2, pp. 165–272. New York: Academic Press 1974Google Scholar
  4. Hamdorf, K., Razmjoo, S.: Prolonged depolarizing afterpotential and its contribution to understanding of photoreceptor function. Biophys. Struct. Mech. 3, 163–170 (1977)Google Scholar
  5. Hillman, P., Hochstein, S., Minke, B.: A visual pigment with two physiological active stable states. Science 175, 1486–1488 (1972)Google Scholar
  6. Hillman, P., Keen, M. E., Winterhager, A. J.: Discussion of selected topics about transduction mechanism in photoreceptors. Biophys. Struct. Mech. 3, 183–189 (1977)Google Scholar
  7. Hochstein, S., Minke, B., Hillman, P.: Antagonistic components of the late receptor potential in the barnacle photoreceptor arising from different stages of the pigment process. J. Gen. Physiol. 62, 105–128 (1973)Google Scholar
  8. Lantz, R. C., Wong, F., Mauro, A.: Lability of prolonged depolarizing afterpotential in Balanus photoreceptors. J. Gen. Physiol. 70, 441–452 (1977)Google Scholar
  9. Minke, B., Hochstein, S., Hillman, P.: Antagonistic process as source of visible-light suppression of afterpotential in Limulus UV photoreceptors. J. Gen. Physiol. 62, 787–791 (1973)Google Scholar
  10. Minke, B., Wu, C.-F., Pak, W. L.: Isolation of light-induced response of central retinular cells from electroretinogram of Drosophila. J. Comp. Physiol. 98, 345–355 (1975a)Google Scholar
  11. Minke, B., Wu, C.-F., Pak, W. L.: Induction of photoreceptor voltage noise in the dark in Drosophila mutant. Nature 258, 84–87 (1975b)Google Scholar
  12. Minke, B.: Drosophila mutant with a transducer defect. Biophys. Struct. Mech. 3, 59–63 (1977)Google Scholar
  13. Muijser, H., Leutscher-Hazelhoff, J. T., Stavenga, D. G., Kuiper, J. W.: Photopigment conversions expressed in receptor potential and membrane resistance of blowfly visual sense cells. Nature 254, 520–522 (1975)Google Scholar
  14. Nolte, J., Brown, J. E.: Ultraviolet induced sensitivity to visible light in ultraviolet receptors of Limulus. J. Gen. Physiol. 59, 186–200 (1972)Google Scholar
  15. Pak, W. L., Lidington, K. J.: Fast electrical potential from a long-lived, long-wavelength photoproduct of fly visual pigment. J. Gen. Physiol. 63, 740–756 (1974)Google Scholar
  16. Pak, W. L., Ostroy, S. E., Deland, M. C., Wu, C.-F.: Photoreceptor mutant of Drosophila: Is protein involved in intermediate step of phototransduction. Science 194, 956–959 (1976)Google Scholar
  17. Tsukahara, Y., Horridge, G. A., Stavenga, D. G.: Afterpotentials in dronefly retinula cells. J. Comp. Physiol. 114, 253–266 (1977)Google Scholar
  18. Wong, F., Wu, C.-F., Mauro, A., Pak, W. L.: Persistance of prolonged light-induced conductance change in arthropods photoreceptors on recovery from anoxia. Nature 264, 661–664 (1976)Google Scholar
  19. Wright, R., Cosens, D.: Blue-adaptation and orange-adaptation in white-eyed Drosophila: evidence that the prolonged afterpotential is correlated with the amount of M 580 in R1–6. J. Comp. Physiol. 113, 105–128 (1977)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Baruch Minke
    • 1
    • 2
  1. 1.Department of PhysiologyHebrew University Hadassah, Medical SchoolJerusalemIsrael
  2. 2.Max-Planck-Institut für biologische KybernetikTübingenFederal Republic of Germany

Personalised recommendations