Abstract
Several murine monoclonal antibodies were raised against purified soluble Plasmodium falciparum antigens from the asexual blood stage. The monoclonal antibodies were purified from ascites by preparative agarose gel electrophoresis and tested for inhibitory activities against P. falciparum in vitro cultures. One monoclonal antibody, HATR 2–4, showed an isolate-specific growth inhibition of P. falciparum in vitro cultures. The antibody reacted in immunoblotting with bands of 250 and 57 kilo dalton (kdalton). Another monoclonal antibody, HATR 2–8, showed growth inhibition of several geographically distinct P. falciparum isolates. HATR 2–8 reacted in immunoblotting with bands of 250 and 74 kdalton. Heating of the antigens destroyed the reactivity of HATR 2–8. The monoclonal antibodies HATR 2–4 and HATR 2–8 probably recognize different epitopes on the same antigen. This antigen circulates in the plasma of some patients with P. falciparum parasitaemia.
Article PDF
Similar content being viewed by others
References
Carlström A, Johansson BG (1983) Agarose gel electrophoresis — immunofixation. Scand J Immunol [Suppl] 10:23–32
Desjardins RE, Canfield CJ, Haynes JD, Chulay JD (1979) Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique. Antimicrob Agent Chemother 16:710–718
Ghosh S, Campbell AM (1986) Multispecific monoclonal antibodies. Immun Today 7:217–22
James MA, Kakoma J, Ristic M, Cagnard M (1985) Induction of protective immunity to Plasmodium falciparum in Saimiri sciureus monkeys with partially purified exoantigens. Infect Immun 49:476–480
Jepsen S (1983) Inhibition of in vitro growth of Plasmodium falciparum by purified antimalarial human IgG antibodies. Scand J Immunol 18:567–571
Jepsen S, Andersen BJ (1981) Immunosorbent isolation of antigens from the culture medium of in vitro cultivated Plasmodium falciparum. Acta Pathol Microbiol Immunol Scand [C] 89:99–103
Jepsen S, Axelsen NH (1980) Antigens and antibodies in Plasmodium falciparum malaria studied by immunoelectrophoretic methods. Acta Pathol Microbiol Immunol Scand [C] 88:263–270
McGregor IA, Wilson RJM (1971) Precipitating antibodies and immunoglobulins in P. falciparum infections in the Gambia, West Africa. Trans R Soc Trop Med Hyg 65:135–151
Perkins ME (1984) Surface proteins of Plasmodium falciparum merozoites binding to the erythrocyte receptor, glycophorin. J Exp Med 160:788–798
Perrin LH, Ramirez E, Lambert PH, Miescher PA (1981) Inhibition of P. falciparum growth in human erythrocytes by monoclonal antibodies. Nature 289:301–303
Reading CL (1982) Theory and methods for immunization in culture and monoclonal antibody production. J Immunol Meth 53:261–291
Rodriquez Da Silva L, Loche M, Dayal R, Perrin LH (1983) Plasmodium falciparum polypeptides released during in vitro cultivation. Bull WHO 61:105–112
Schapira A, Fogh S, Jepsen S, Pedersen NS (1984) Detection of antibodies to malaria: comparison of results with ELISA, IFAT and crossed immunoelectrophoresis. Acta Pathol Microbiol Immunol Scand [B] 92:299–304
Trager W, Jensen JB (1976) Human malaria parasites in continuous culture. Science 193:673–675
Wilson RJM (1980) Serotyping Plasmodium falciparum malaria with S-antigens. Nature 284:451–452
Wilson RJM, McGregor IA, Hall P, Williams K, Bartholomew R (1969) Antigens associated with Plasmodium falciparum infections in man. Lancet 11:201–205
Wilson RJM, McGregor IA, Wilson ME (1973) The stability and fractionation of malarial antigens from the blood of Africans infected with Plasmodium falciparum. Int J Parasitol 3:511–520
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jakobsen, P.H., Jepsen, S. & Agger, R. Inhibitory monoclonal antibodies to soluble Plasmodium falciparum antigens. Parasitol Res 73, 518–523 (1987). https://doi.org/10.1007/BF00535326
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00535326