Abstract
Two species of trypanosomatid flagellates, Trypanosoma cruzi and Blastocrithidia triatomae which develop in the intestine of Triatoma infestans, were incubated with lectin-gold conjugates to demonstrate sugar components of the cell surface with the electron microscope. T. cruzi, strain “Chile 5” (zymodeme 1), bound SBA-gold, revealing N-acetyl-galactosamine and/or galactose residues. WGA-BSA-gold, specific for N-acetylglucosamine, did not bind to the surface of this flagellate. On the other hand, N-acetyl-glucosamine bound WGA-BSA-gold on the cell membrane of epimastigotes and cysts of B. triatomae, whereas SBA-gold was not bound by flagellates which were attached to the midgut epithelium and the rectum cuticle. Among other criteria, these differences in lectin binding may facilitate the discrimination of the two parasite species in the vector of Chagas' disease.
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References
Allen AK, Neuberger A, Sharon N (1973) The purification, composition and specificity of wheat germ agglutinin. Biochem 1131:155–162
Böker CA, Schaub GA (1984) Scanning electron microscopic studies of Trypanosoma cruzi in the rectum of its vector Triatoma infestans. Z Parasitenkd 70:459–469
Cerisola JA, Del Prado CE, Rowedder R, Bozzini JP (1971) Blastocrithidia triatomae n.sp. found in Triatoma infestans from Argentina. J Protozool 18:503–506
Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature 241:20–22
Goldstein IJ, Hammarström S, Sundblad G (1975) Precipitation and carbohydrate-binding specificity studies on wheat germ agglutinin. Biochim Biophys Acta 405:53–61
Horisberger M, Rossett J (1977) Colloidal gold, a useful marker for transmission and scanning electron microscopy. J Histochem Cytochem 25:295–305
Kolb-Bachofen V, Kempka G (1985) Galactose specific receptors on liver cell: I. Hepatocyte and liver macrophage receptors differ in their membrane anchorage. Biochim Biophys Acta 847:108–114
Lis H, Sela BA, Sachs L, Sharon N (1970) Specific inhibition by N-acetyl-D-galactosamine of the interaction between soybean agglutinin and animal cell surfaces. Biochim Biophys Acta 211:582–587
Marinkelle CJ, Vallejo GA, Schottelius J, Guhl F, de Sanchez N (1986) The affinity of the lectins Ricinus communis and Glycine maxima to carbohydrates on the cell surface of various forms of Trypanosoma cruzi and Trypanosoma rangeli, and the application of these lectins for the identification of T. cruzi in the feces of Rhodnius prolixus. Acta Trop (Basel) 43:215–223
Nagamura Y, Kolb H (1980) Presence of a lectin-like receptor for D-galactose on rat peritoneal macrophages. FEBS Lett 115:59–62
Nogueira N, Unkeless J, Cohn Z (1982) Specific glycoprotein antigens on the surface of insect and mammalian stages of Trypanosoma cruzi. Proc Natl Acad Sci USA 79:1259–1263
Reduth D (1986) In vitro Kultivierung, Entwicklungszyklus und Ultrastruktur von Blastocrithidia triatomae Cerisola et al. 1971 (Trypanosomatidae, Kinetoplastida, Protozoa). Inaug Diss, University of Freiburg, FRG
Roos PH, Hartmann H-P, Schlepper-SchÄfer J, Kolb H, KolbBachofen V (1985) Galactose specific receptors on liver cell: II. Characterization of the purified receptor from macrophages reveals no structural relationship to the hepatocyte receptor. Biochim Biophys Acta 847:115–121
Schaub GA (1982) Parasite/host interrelationships of Blastocrithidia triatomae (Trypanosomatidae) and Reduviids. Zentralbl Bakteriol I Abt Referate 277:101–102
Schaub GA, Böker CA (1986) Scanning electronmicroscopic studies of Blastocrithidia triatomae (Trypanosomatidae) in the rectum of Triatoma infestans (Reduviidae). J Protozool 33:266–270
Schaub GA, Pretsch M (1981) Ultrastructural studies on the exocystment of Blastocrithidia triatomae (Trypanosomatidae). Trans R Soc Trop Med Hyg 75:168–171
Schaub GA, Schottelius J (1984) Identification of trypanosomes isolated from reduviidae from North Chile. Z Parasitenkd 70:3–9
Schaub GA, Berndt CA, Neukirchen K (1982) Raster- und durchlicht-elektronenmikroskopische Untersuchungen zur Besiedlung und zur Anheftung von Blastocrithidia triatomae und Trypanosoma cruzi (Protozoa, Trypanosomatidae) im Darm von Triatoma infestans (Insecta, Reduviidae). Verh Dtsch Zool Ges 1982:286
Schottelius J, Uhlenbruck G (1983) Comparative studies of Trypanosoma cruzi and T. cruzi-like stocks from different South American countries using lectins. Z Parasitenkd 69:727–736
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This work is part of a doctoral thesis at the Universität Düsseldorf, it has been supported by a grant from Nordrhein-Westfalen and by Deutsche Forschungsgemeinschaft (SCHA 339/1-5)
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Zimmermann, D., Peters, W. & Schaub, G.A. Differences in binding of lectin-gold conjugates by Trypanosoma cruzi and Blastocrithidia triatomae (Trypanosomatidae) in the intestine of Triatoma infestans (Reduviidae). Parasitol Res 74, 5–10 (1987). https://doi.org/10.1007/BF00534924
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DOI: https://doi.org/10.1007/BF00534924