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Number of nucleoli and coiled bodies and distribution of fibrillar centres in differentiating Purkinje neurons of chick and rat cerebellum

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Abstract

We used differentiating chick and rat Purkinje cells to investigate in homologous neurons the influence of the number of nucleolar organizer regions (two in the chick and six in the rat) on the behaviour of the nucleolus and coiled bodies. We employed specific silver-staining methods on smear preparations and on semithin and ultrathin sections. In chick Purkinje cells the number of nucleolar silver-staining granules increased from 15.7±3 (mean±SD) at embryonic day 13 to 23.8±3 at post-hatching day 7. These nucleolar granules were unevenly distributed between the two nucleoli of binucleolated cells. Electron-microscopic cytochemistry showed that nucleolar granules are equivalent to the fibrillar centres with their associated shell of dense fibrillar component. A reduction in the number of nucleoli was found during the differentiation of both chick and rat Purkinje cells, although in mature cells the average number of nucleoli per cell was higher in the chick (1.60) than in the rat (1.07). The number of coiled bodies decreased from 1.33 in newborn rats to 0.47 at postnatal day 90 in the rat. Coiled bodies were not observed in homologous chick Purkinje cells. The dynamic behaviour of nucleoli and coiled bodies during neuronal differentiation and the relationship of these two nuclear organelles with the number of nucleolar organizer regions is discussed.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, University of Cantabria, Avda. Cardenal Herrera Oria s/n, E-39011, Santander, Spain

    M. Lafarga, M. A. Andrés, C. Fernández-Viadero, J. Villegas & M. T. Berciano

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  1. M. Lafarga
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  2. M. A. Andrés
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  3. C. Fernández-Viadero
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  4. J. Villegas
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  5. M. T. Berciano
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Lafarga, M., Andrés, M.A., Fernández-Viadero, C. et al. Number of nucleoli and coiled bodies and distribution of fibrillar centres in differentiating Purkinje neurons of chick and rat cerebellum. Anat Embryol 191, 359–367 (1995). https://doi.org/10.1007/BF00534689

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