Abstract
We have studied the process of preparing a silica support with anhydride groups. The process includes introducing vinyl groups onto the surface with subsequent radiative copolymerization of maleic anhydride with them. The maximum degree of grafting of the functional groups of the matrix occurs for γ-irradiation doses of 20–30 Mrad. The carboxyl groups grafted to the aerosil surface, formed upon hydrolysis of the support, can be converted to anhydride by calcination at 403 K for 3 h.
This is a preview of subscription content, log in via an institution to check access.
Literature cited
V. A. Tertykh and V. V. Yanishpol'skii, “Chemical aspects of immobilization of enzymes in an inorganic matrix,” Adsorpts. Adsorb., No. 8, 3–27 (1980).
V. P. Kumarev, A. S. Rait, V. K. Rait, and R. I. Salganik, “Immobilization of proteins and enzymes on aldehydosiliochromes,” Bioorg. Khim., 2, No. 5 (1975).
E. A. Glazunov and A. M. Chernaenko, “Method of addition of aliphatic aldehyde to the surface of large-pore glass,” Prikl. Biokhim. Mikrobiol., 21, No. 4, 533–536 (1985).
G. V. Lyubinskii, V. V. Yanishpol'skii, V. A. Tertykh et al., “Properties of urease immobilized on silochrome using disulfide bonds,” Ukr. Biokhim. Zh., 56, No. 1, 24–27 (1984).
V. P. Borisova, I. D. Stal'naya, I. I. Menyailova et al., “Immobilization of enzymes on supports with azide groups,” Prikl. Biokhim. Mikrobiol., 14, No. 5, 709–714 (1978).
L. A. Nakhapetyan, N. S. Golovina, and N. B. Geodakyan, “Method for obtaining immobilized enzymes,” Inventor's Certificate 644760 USSR, MKI3 C 07 C 7/02, Publ. 30.01.79, Byull. No. 4.
K. K. Popkov, “Reaction of silanes with hydrogen chloride,” Zh. Prikl. Spektrosk., 13, No. 2, 318–321 (1970).
I. E. Neimark, G. B. Budkevich, T. N. Burushkina, et al., “Chemical nature of the surface of silicas and its effect on their adsorption and technological properties,” in: Natural Sorbents [in Russian], Nauka, Moscow (1967), pp. 56–77.
V. K. Savel'ev, A. A. Chuiko, V. A. Tertykh, et al., “Method for obtaining organofunctional silicas,” Inventor's Certificate USSR, MKI3 C 09 C 3/00, C 08 G 31/24, Publ. 26.03.71, Byull. No. 12.
J. L. Lang, W. Pavelich, and H. D. Clarey, “Homopolymerization of maleic anhydride. 1. Preparation of the polymer,” J. Polym. Sci. A, 1, No. 4, 1123–1136 (1963).
L. Goldstein, “Water-insoluble proteolytic enzymes,” Meth. in Enzymol., 19, 948–949 (1970).
F. A. Mason, “A simple method for the preparation of maleic anhydride,” J. Chem. Soc. A, No. 3, 700–701 (1930).
N. N. Korenman, Photometric Analysis. Methods for Determination of Organic Compounds [in Russian], Khimiya, Moscow (1975).
N. Ramadan and J. Porath, “Iron(3+)-hydroxamate as immobilized metal affinity adsorbent for protein chromatography,” J. Chromatogr., 321, No. 1 (1985).
B. F. Erlanger, N. Kokowsky, and W. Cogen, “The preparation and properties of two new chromogenic substrates of trypsin,” Arch. Biochem. Biophys., 95, No. 1 (1961).
G. V. Lyubinskii, “Interaction between optimal pH for protein immobilization and their isoelectric points,” Ukr. Biokhim. Zh., 56, No. 4, 390–394 (1984).
Author information
Authors and Affiliations
Additional information
Translated from Teoreticheskaya i Éksperimental'naya Khimiya, Vol. 25, No. 6, pp. 750–753, November–December, 1989.
Rights and permissions
About this article
Cite this article
Yanishpol'skii, V.V., Lyubinskii, G.V. & Tertykh, V.A. Silica Matrix with anhydride groups for immobilization of enzymes. Theor Exp Chem 25, 690–693 (1989). https://doi.org/10.1007/BF00534456
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00534456