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Immunocytochemical demonstration of intestinal alkaline phosphatase

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Summary

In the present study a histochemical and an immunocytochemical method have been employed for the demonstration of alkaline phosphatase in the duodenum of different species at light and electron microscopic level. The commercially obtained intestinal alkaline phosphatase from the calf, which was used for the production of specific antiserum, was investigated by immunodiffusion, immunoelectrophoresis and polyacrylamide gel electrophoresis. For immunocytochemical demonstration of the enzyme a triple-layer-PAP-method has been used. Both methods employed showed identical distribution patterns of the enzyme within the enterocytes while in goblet cells the antigen could only be demonstrated by immunochemistry. Our findings are discussed with special reference to the role of the Golgi field and the function of the goblet cells.

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Authors and Affiliations

  1. Institute of Pathology, University of Hamburg, Federal Republic of Germany

    H. Lietz, W. Böcker & M. Gröblinghoff

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  1. H. Lietz
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  2. W. Böcker
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  3. M. Gröblinghoff
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This work was supported by the Deutsche Forschungsgemeinschaft

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Lietz, H., Böcker, W. & Gröblinghoff, M. Immunocytochemical demonstration of intestinal alkaline phosphatase. Histochemistry 42, 181–191 (1974). https://doi.org/10.1007/BF00533269

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  • DOI: https://doi.org/10.1007/BF00533269

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