, Volume 69, Issue 2, pp 169–179 | Cite as

Demonstration of enkephalin-, substance p- and glutamate decarboxylase-like immunoreactivity in cultured cells derived from newborn rat neostriatum

  • P. Panula
  • P. Emson
  • J. -Y. Wu


The presence of cells exhibiting leucine-enkephalin-, substance P- and glutamate decarboxylase-like immunoreactivity was demonstrated in dissociated cultures from newborn rat neostriatum. The size and shape of the enkephalin-immunoreactive cells varied, but they were generally larger than substance P- and glutamate decarboxylase-immunoreactive cells, which formed relatively uniform cell populations. Cells of apparently non-neuronal origin did not show any immunoreactivity. It is unlikely that enkephalin is present in the same cells that contain substance P or glutamate decarboxylase because of norphological differences between these cells. The possible coexistence of substance P and glutamate decarboxylase in the same cells however, could not be excluded. The results of this study confirm that the cell bodies of neurons containing three possible neurotransmitters are located in the neostriatum.


Public Health Glutamate Cell Population Cell Body Glutamate Decarboxylase 
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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • P. Panula
    • 1
  • P. Emson
    • 2
  • J. -Y. Wu
    • 3
  1. 1.Department of AnatomyUniversity of HelsinkiHelsinki 17Finland
  2. 2.MRC Neurochemical Pharmacology Unit, Department of PharmacologyMedical SchoolCambridgeEngland
  3. 3.Department of Cell Biology, Baylor College of MedicineTexas Medical CenterHoustonUSA

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