Zeitschrift für Krebsforschung

, Volume 63, Issue 2, pp 184–208 | Cite as

Cytological studies of tumors, XXVII. The chromosomes of thirty human tumors

  • Sajiro Makino
  • Takaaki Ishihara
  • Akira Tonomura
Article

Summary

The chromosome conditions were investigated in thirty cases of primary human tumors in two types of material, solid and effusion, by the use of the water-pretreatment squash method.

The number of chromosomes of human tumors shows a pronounced variation within a wide range, but there is always present in each tumor a stem-line (or lines) of tumor cells occurring most frequently with a characteristic number-mode (or modes) different from the normal number, and a specific idiogram (or idiograms) clearly distinguishable from those of other tumors as well as from that of normal tissue. There are indications that together with the numerical changes of chromosomes, the human tumors might have undergone many possible structural and mutational changes of the stemline chromosomes.

The tumors investigated here exhibit a variety of stemline chromosome numbers. The tumors characterized by hyperdiploid stemline number are most frequent amongst thirty cases studied.

Different tumors which originated from the same organ are characterized by different stemline chromosome patterns. This seems to suggest that the genetic pattern which is closely correlated with the property of the tumor may not be identical in each tumor, even though they originated from the same organ.

Keywords

Public Health Tumor Cell Cancer Research Normal Tissue Chromosome Number 

Zusammenfassung

Untersucht wurde das Verhalten der Chromosomen bei 30 menschlichen Tumoren, die teils in der soliden Form vorlagen, teils wurden Tumorzellen aus Exsudaten untersucht. Die Chromosomenzahl zeigte eine große Variationsbreite. In jedem Tumor sind aber eine oder mehrere Stammlinien von am häufigsten vorkommenden Zellen nachweisbar mit einer von der Normalzelle abweichenden charakteristischen Chromosomenzahl und einem spezifischen Idiogramm, das sich deutlich von dem anderer Tumoren wie auch von dem anderer Zellen unterscheidet. Am häufigsten sind hyperdiploide Chromosomensätze. Verschiedene Tumoren desselben Organs sind durch ein verschiedenes Chromosomenmuster der Stammlinien gekennzeichnet.

Offenbar haben Tumoren zugleich mit den zahlenmäßigen Veränderungen der Chromosomen auch strukturelle Veränderungen und Mutationen der Chromosomen mitgemacht.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature

  1. Hansen-Melander, E., S. Kullander and Y. Melander: Chromosome analysis of a human ovarian cystocarcinoma in the ascites form. J. Nat. Cancer Inst. 16, 1067–1081 (1956).Google Scholar
  2. Hauschka, T. S.: Cell population studies on mouse ascites tumors. Trans. N.Y. Acad. Sci., Ser. II 16, 64–73 (1953).Google Scholar
  3. Hsu, T. C.: Mammalian chromosomes in vitro. IV. Some human neoplasms. J. Nat. Cancer Inst. 14, 905–933 (1954).Google Scholar
  4. Lecture at Gordon Cancer Research Conference, New London, N. H., 1957.Google Scholar
  5. Hsu, T. C., and O. Klatt: Mammalian chromosomes in vitro. IX. On genetic polymorphism in cell populations. J. Nat. Cancer Inst. 21, 437–473 (1958).Google Scholar
  6. Ising, U., and A. Levan: The chromosomes of two highly malignant human tumours. Acta path. microbiol. scand. 40, 13–24 (1957).Google Scholar
  7. Koller, P. C.: Abnormal mitosis in tumors. Brit. J. Cancer 1, 38–47 (1947).Google Scholar
  8. Levan, A.: Chromosomes in cancer tissue. Ann. N.Y. Acad. Sci. 63, 774–780 (1956a)Google Scholar
  9. ——: Self-perpetuating ring chromosomes in two human tumors. Hereditas (Lund) 42, 366–372 (1956b).Google Scholar
  10. Makino, S.: A review of the chromosome numbers in animals, 2. edit. Tokyo 1956.Google Scholar
  11. —— The chromosome cytology of the ascites tumors of rats, with special reference to the concept of the stemline cell. Int. Rev. Cytol. 6, 25–84 (1957a).Google Scholar
  12. ——: Cytological studies of tumors, XX. A chromosome analysis in the MTK-IV tumor, a hypotriploid rat ascites tumor. J. Fac. Sci. Hokkaido, Ser. VI Zool. 13, 263–267 (1957b).Google Scholar
  13. Makino, S., and M. Sasaki: Ditto, XXI. A comparative ideogram study of the Yoshida sarcoma and its subline derivatives. J. nat. Cancer Inst. 20, 465–487 (1958).Google Scholar
  14. ——: The chromosome number of man. A preliminary report. Proc. jap. Acad. 35, 99–104 (1959).Google Scholar
  15. Manna, G. K.: A study on chromosomes of the human nonneoplastic and neoplastic uterine tissues. Proc. Intern. Genetics Symposia, 1956, p. 182–187. 1956.Google Scholar
  16. A study on the chromosome number of human neoplastic uterine cervix tissue. Proc. Zool. Soc. Calcutta, Mookerjee Memor., 95–112 (1957).Google Scholar
  17. Sasaki, M.: Cytological studies of tumors. XXIV. An azo-dye induced ascites hepatoma of the rat, etc. Gann 49, 331–340 (1958).Google Scholar
  18. Tjio, J. H., and A. Levan: The chromosome number of man. Hereditas (Lund) 42, 1–6 (1956a).Google Scholar
  19. —: Comparative ideogram analysis of the rat and the Yoshida rat sarcoma. Hereditas (Lund) 42, 218–234 (1956b).Google Scholar
  20. Tonomura, A., and M. Sasaki: Cytological studies of tumors. XIX. A chromosome survey of the MTK-sarcoma II and III after several years of serial transfers. J. Fac. Sci. Hokkaido, Ser. VI Zool. 13, 332–337 (1957).Google Scholar
  21. Wakabayashi, M., and T. Ishihara: Cytological studies of tumors XXVI. Chromosome analysis of a human mammary carcinoma. Cytologia (Tokyo) 23, 341–348 (1958).Google Scholar

Copyright information

© Springer-Verlag 1959

Authors and Affiliations

  • Sajiro Makino
    • 1
  • Takaaki Ishihara
    • 1
  • Akira Tonomura
    • 1
  1. 1.Zoological InstituteHokkaido UniversitySapporoJapan

Personalised recommendations